Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,965,828	G→A	P122S (CCG→TCG)	flhB ←	flagellar biosynthesis protein FlhB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,965,828	0	G	A	100.0%	81.0 / NA	25	P122S (CCG→TCG)	flhB	flagellar biosynthesis protein FlhB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/11);  total (14/11)

AAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCC  >  NC_000913/1965709‑1965956                                                                                                                        |                                                                                                                                 agaCCCCGTCACGCTGCCAACCAGAATGCTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGACTGCAAGGATTTg                                                                                                                     <  2:293436/131‑1 (MQ=255)  aaCCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGACTGCAAGGATTTGc                                                                                                                    <  1:76051/133‑1 (MQ=255)       cGTCACGCTGCCAACAAGGATGCTTTTCAAGATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGACTGCAAGGATTTGCCGCTa                                                                                                               <  1:210438/133‑1 (MQ=255)                    aCCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGACTGCAAGGATTTGCCGCTAAATACCAGCCCTc                                                                                                  <  1:124699/133‑1 (MQ=255)                           tGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGACTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCAt                                                                                           >  1:585002/1‑133 (MQ=255)                              ttttCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGACTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGAc                                                                                        >  1:154708/1‑133 (MQ=255)                                   aaaaTTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGACTGCAAGGATTTGCCGTTAAATACAAGCACTACCAGCACGACCGgag                                                                                   >  1:301985/1‑133 (MQ=255)                                      aTTGCTTTAAGTAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGACTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGaaa                                                                                <  2:585002/133‑1 (MQ=255)                                                gCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGACTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGccac                                                                      <  2:154708/133‑1 (MQ=255)                                                    cTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGACTGCAAGGATTTGCCGCTAAATGCCAGCCCTGCCAGCATAACCGGAGAGATGAGCGCCACCAGc                                                                  >  2:164079/1‑133 (MQ=255)                                                         cgcCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGACTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGcaccac                                                             >  1:376648/1‑133 (MQ=255)                                                               tCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGACTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCt                                                       >  2:616762/1‑133 (MQ=255)                                                                           aCATCCGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGACTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGgcag                                           <  2:376648/133‑1 (MQ=255)                                                                             aTCCGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGACTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGgcagca                                         >  1:70281/1‑133 (MQ=255)                                                                                  tttAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGACTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCagcagc                                       >  1:599629/1‑130 (MQ=255)                                                                                  tttAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGACTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCagcagc                                       <  2:599629/130‑1 (MQ=255)                                                                                  tttAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGACTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGcc                                    <  1:616762/133‑1 (MQ=255)                                                                                      aTGCCCGGTAGCGGGTTGAGTTTTAAAACCTTTGACTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCa                                <  2:301985/133‑1 (MQ=255)                                                                                         cccGGTAGCGGGTTGAGTTTGGAAAACTTCGACTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATgg                             >  2:452520/1‑133 (MQ=255)                                                                                            ggTAGCGGGTTGAGTTTGGAAAACTTCGACTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCtt                          >  2:118111/1‑133 (MQ=255)                                                                                                   ggTTGAGTTTGGAAAACTTCGACTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGAt                   <  1:118111/133‑1 (MQ=255)                                                                                                        aGTTTGGAAAACTTCGACTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCGGCAGCGCCAGCATGGCTTCTCAGATcagca              >  1:265921/1‑133 (MQ=255)                                                                                                               aaaaCTTCGACTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGaataat       >  2:22971/1‑133 (MQ=255)                                                                                                                    ttCGACTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGTTACTCTGATCAGCAGGATAATCTgac  >  2:289509/1‑131 (MQ=255)                                                                                                                    ttCGACTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGcc  >  2:602207/1‑133 (MQ=255)                                                                                                                        |                                                                                                                                 AAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCC  >  NC_000913/1965709‑1965956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑