Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,985,500	T→C	N210S (AAC→AGC)	araF ←	arabinose ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,985,500	0	T	C	100.0%	46.9 / NA	14	N210S (AAC→AGC)	araF	arabinose ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/6);  total (8/6)

ACCTTCCGTCGCGCGTACGCCGCCCAGCACGGTGCTGTCGTTCATACCGACGATCAGCCAATGTTTAACTTCCGGATGTTGAACCAGCATTGAGTTGGCAGCGTCAAATGCCCCCGGGATGTCGTTAGATTTGGTAGGTACCTGATAAATTTGTTTTTCCGGGAATCCGGCCGCTTTCAGCGCATCCATAGATCC  >  NC_000913/1985406‑1985600                                                                                               |                                                                                                     aCCTTCCGTCGCGCGTACGCCGCCCAGCACGGTGCTGTCGTTCATACCGACGATCAGCCAATGTTTAACTTCCGGATGTTGAACCAGCATTGAGCTGGCAGCGTCAAATGCCCCCGGGATGTCGTTAGATTTg                                                                >  1:491869/1‑133 (MQ=255)           gcgcgTACGCCGCCCAGCACGGTGCTGTCGTTCATACCGACGATCAGCCAATGTTTAACTTCCGGATGTTGAACCAGCATTGAGCTGGCAGCGTCAAATGCCCCCGGGATGTCGTTAGATTTGGTAGGTACCt                                                      >  1:29448/1‑133 (MQ=255)                tACGCCGCCCAGCACGGTGCTGTCGTTCATACCGACGATCAGCCAATGTTTAACTTCCGGATGTTGAACCAGCATTGAGCTGGCAGCGTCAAATGCCCCCGGGATGTCGTTAGATTTGGTAGGTACCTGATaa                                                 <  1:67394/133‑1 (MQ=255)                   gccgccCAGCACGGTGCTGTCGTTCATACCGACGATCAGCCAATGTTTAACTTCCGGATGTTGAACCAGCATTGAGCTGGCAGCGTCAAATGCCCCCGGGATGTCGTTAGATTTGGTAGGTACCTGATAAAtt                                              >  1:511068/1‑133 (MQ=255)                                           cATACCGACGATCAGCCAATGTTTAACTTCCGGATGTTGAACCAGCATTGAGCTGGCAGCGTCAAATGCCCCCGGGATGTCGTTAGATTTGGTAGGTACCTGCTAAATTTTTTTTTCAGGGAATCCGGCCGCt                      >  1:307729/1‑133 (MQ=255)                                           cATACCGACGATCAGCCAATGTTTAACTTCCGGATGTTGAACCAGCATTGAGCTGGCAGCGTCAAATGCCCCCGGGATGTCGTTAGATTTGGTAGGTACCTGATAAATTTGTTTTTCCGGGAATCCGGCCGCt                      >  1:274899/1‑133 (MQ=255)                                                cgacgaTCAGCCAATGTTTAACTTCCGGATGTTGAACCAGCATTGAGCTGGCAGCGTCAAATGCCCCCGGGATGTCGTTAGATTTGGTAGGTACCTGATAAATTTGTTTTTCCGGGAATCCGGCCGCTTTCAg                 <  2:274899/133‑1 (MQ=255)                                                  acgaTCAGCCTATGTGTAACTTCCCGATGTTGAACCAGCATTGAGCTGGCAGCGTCAAATGCCCCCGGGATGTCGTTAGATTTGGTAGGTACCTGATAAATTTGTTTTTCCGGGAATCCGGCCGCTTTCAgcg               <  2:258768/133‑1 (MQ=255)                                                  acgaTCAGCCAATGTTTAACTTCCGGATGTTGAACCAGCATTGAGCTGGCAGCGTCAAATGCCCCCGGGATGTCGTTAGATTTGGTAGGTACCTGATAAATTTGTTTTTCCGGGAATCCGGCCGCTTTCAgcg               <  2:511068/133‑1 (MQ=255)                                                  acgaTCAGCCAATGTTTAACTTCCGGATGTTGAACCAGCATTGAGCTGGCAGCGTCAAATGCCCCCGGGATGTCGTTAGATTTGGTAGGTACCTGATAAATTTGTTTTTCCGGGAATCCGGCCGCTTTCAg                 <  1:411071/131‑1 (MQ=255)                                                  acgaTCAGCCAATGTTTAACTTCCGGATGTTGAACCAGCATTGAGCTGGCAGCGTCAAATGCCCCCGGGATGTCGTTAGATTTGGTAGGTACCTGATAAATTTGTTTTTCCGGGAATCCGGCCGCTTTCAg                 >  2:411071/1‑131 (MQ=255)                                                   cgaTCAGCTAATGTTCAAGTTCTGGATGTTGAACCAGCATTGAGCTGGCAGCGTCAAATGCCCCCGGGATGTCGTTAGATTTGGTAGGTACCTGATAAATTTGTTTTTCCGGGAATCCGGCCGCTTTCAgcgc              <  1:303235/133‑1 (MQ=255)                                                               gTTTAACTTCCGGATGTTGAACCAGCATTGAGCTGGCAGCGTCAAATGCCCCCGGGATGTCGTTAGATTTGGTAGGTACCTGATAAATTTGTTTTTCCGGGAATCCGGCCGCTTTCAGCGCATCCATAGATcc  >  2:494788/1‑133 (MQ=255)                                                               gTTTAACTTCCGGATGTTGAACCAGCATTGAGCTGGCAGCGTCAAATGCCCCCGGGATGTCGTTAGATTTGGTAGGTACCTGATAAATTTGTTTTTCCGGGAATCCGGCCGCTTTCAGCGCATCCATAGATcc  >  2:538333/1‑133 (MQ=255)                                                                                               |                                                                                                     ACCTTCCGTCGCGCGTACGCCGCCCAGCACGGTGCTGTCGTTCATACCGACGATCAGCCAATGTTTAACTTCCGGATGTTGAACCAGCATTGAGTTGGCAGCGTCAAATGCCCCCGGGATGTCGTTAGATTTGGTAGGTACCTGATAAATTTGTTTTTCCGGGAATCCGGCCGCTTTCAGCGCATCCATAGATCC  >  NC_000913/1985406‑1985600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑