Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,041,215	T→C	intergenic (+97/‑160)	msrQ → / → zinT	periplasmic protein‑L‑methionine sulfoxide reductase heme binding subunit/metal‑binding protein ZinT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,041,215	0	T	C	100.0%	118.4 / NA	33	intergenic (+97/‑160)	msrQ/zinT	periplasmic protein‑L‑methionine sulfoxide reductase heme binding subunit/metal‑binding protein ZinT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/18);  total (15/18)

GCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACA  >  NC_000913/2041087‑2041344                                                                                                                                 |                                                                                                                                  gCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGccatc                                                                                                                               <  2:638576/133‑1 (MQ=255)      cAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGGATCAGGTAATGTTTAGTTTGCTATCAACCt                                                                                                                          >  1:204959/1‑133 (MQ=255)         gTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGCCATCAACCTCaa                                                                                                                       <  1:513133/133‑1 (MQ=255)                                    cATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATG‑TTCGTTTGCCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCaa                                                                                           >  2:126410/1‑133 (MQ=255)                                     atatTGCCGAGAAAGGACGCGTTATCCCTGCCGGATGTGGCCCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGCCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCaa                                                                                           <  2:234558/133‑1 (MQ=255)                                     atatTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGCCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCaa                                                                                           <  2:446121/133‑1 (MQ=255)                                      tatTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGCCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAAt                                                                                          >  2:157960/1‑133 (MQ=255)                                      tatTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGCCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAAt                                                                                          >  1:624928/1‑133 (MQ=255)                                      tatTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGCCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAAt                                                                                          >  1:623992/1‑133 (MQ=255)                                       atTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGCCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATa                                                                                         <  2:145051/133‑1 (MQ=255)                                          gCCGACAAAGTACGCTTTATCCCTCCCGTATGTGGCGCGAGTGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGCCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAaga                                                                                      <  2:204959/133‑1 (MQ=255)                                          gCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGCCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAaga                                                                                      <  2:450739/133‑1 (MQ=255)                                             gACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGCCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAagatag                                                                                   <  1:342207/133‑1 (MQ=255)                                                 aaGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGCCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATaa                                                                               >  2:560362/1‑133 (MQ=255)                                                                 tGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGCCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGc                                                               >  2:141348/1‑133 (MQ=255)                                                                           ggCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGCCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCg                                                     >  1:434463/1‑133 (MQ=255)                                                                           ggCGCGAGCGACCTATCCGGCATACACACATCTCATCAGGTAATGTTTCGTTTGCCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCg                                                     <  2:187930/133‑1 (MQ=255)                                                                                 aGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGCCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCt                                               >  2:389347/1‑133 (MQ=255)                                                                                 aGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGCCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCt                                               <  2:623992/133‑1 (MQ=255)                                                                                              gCATACACAGATCGCATCAGGTTATGTTTCGTTTGCCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCa                                  <  1:389347/133‑1 (MQ=255)                                                                                                   cacaGATCGCATCAGGTTATGTTTCGTTTGCCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTg                             <  1:560362/133‑1 (MQ=255)                                                                                                     caGATCGCATCAGGTTATGTTTCGTTTGCCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCt                           <  1:157960/133‑1 (MQ=255)                                                                                                        aTCGCATCAGGTTATGTTTCGTTTGCCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCt                           <  2:379408/130‑1 (MQ=255)                                                                                                        aTCGCATCAGGTTATGTTTCGTTTGCCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCt                           >  1:379408/1‑130 (MQ=255)                                                                                                             aTCAGGTTATGTTTCGTTTGCCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTa                   <  2:624928/133‑1 (MQ=255)                                                                                                              tCAGGTTATGTTTCGTTTGCCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTAc                  >  2:338499/1‑133 (MQ=255)                                                                                                                 ggTTATG‑TTCGTTTGCCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAata              <  1:126410/133‑1 (MQ=255)                                                                                                                  gTTATGTTTCGTTTGCCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAata              >  1:48136/1‑133 (MQ=255)                                                                                                                  gTTATGTTTCGTTTGCCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAata              >  2:568350/1‑133 (MQ=255)                                                                                                                      tGTTTCGTTTGCCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAAc          >  1:436717/1‑133 (MQ=255)                                                                                                                           cGTTTGCCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTac     <  1:431358/133‑1 (MQ=255)                                                                                                                           cGTTTGCCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTac     <  1:371349/133‑1 (MQ=255)                                                                                                                            gTTTGCCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAata              >  1:502139/1‑123 (MQ=255)                                                                                                                            gTTTGCCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAata              <  2:502139/123‑1 (MQ=255)                                                                                                                              ttGCCATCGACCTCATATGACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTAcaca  <  1:300290/133‑1 (MQ=255)                                                                                                                                 |                                                                                                                                  GCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACA  >  NC_000913/2041087‑2041344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑