Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,048,107	G→T	G1057V (GGC→GTC)	yeeJ →	inverse autotransporter adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,048,107	0	G	T	100.0%	73.5 / NA	23	G1057V (GGC→GTC)	yeeJ	inverse autotransporter adhesin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (9/14);  total (9/14)

CACGCATATGATCATGGCTCGTCTGGCTAACAGCAATGTCAGCGATGCACAGCCAATGACGTTTGTGGCGGATAAAGACAGAGCGGTTGTCGTTTTGCAAACATCGAAAGCGGAAATCATTGGGAATGGCGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGC‑GGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTA  >  NC_000913/2047979‑2048232                                                                                                                                 |                                                                                                                               cACGCATATGATCATGGCTCGTCTGGCTAACAGCAATGTCAGCGATGCACAGCCAATGACGTTTGTGGCGGATAAAGACAGAGCGGTTGTCGTTTTGCAAACATCGAAAGCGGAAATCATTGGGAATGTCGTg                                                                                                                            <  1:52555/133‑1 (MQ=255)        atGATCATGGCTCGTCTGGCTAACAGCAATGTCAGCGATGCACAGCCAATGACGTTTGTGGCGGATAAAGACAGAGCGGTTGTCGTTTTGCAAACATCGAAAGCGGAAATCATTGGGAATGTCGTGGATgaga                                                                                                                     <  2:576507/133‑1 (MQ=255)             cATGGCTCGTCTGGCTAACAGCAATGTCAGCGATGCACAGCCAATGACGTTTGTGGCGGATAAAGACAGAGCGGTTGTTGTTTTGCAAACATCGAAAGCGGAAATCATTGGGAATGTCGTGGATGAGACAAct                                                                                                                <  1:370475/133‑1 (MQ=255)                ggCTCGTCTGGCTAACAGCAATGTCAGCGATGCACAGCCAATGACGTTTGTGGCGGGGAAAAAAAAAGGGGGTGTCGGTTTTTGAACAACGAAAAGGGGAAACATTTGGGAAGGTGTGGGTGGGaaaaaactt                                                                                                             >  2:104288/1‑128 (MQ=255)                     gTCTGGCTAACAGCAATGTCAGCGATGCACAGCCAATGACGTTTGTGGCGGATAAAGACAGAGCGGTTGTCGTTTTGCAAACATCGAAAGCGGAAATCATTGGGAATGTCGTGGATGAGACAACTCTGACAGc                                                                                                        <  2:392263/133‑1 (MQ=255)                      tCTGGCTAACAGCAATGTTAGCCATGCACACCCAATGACGTTTGTGGCGGATAAAGACAGAGCGGTTGTCGTTTTGCAAACATCGAAAGCGGAAATCATTGGGAATGTCGTGGATGAGACAACTCTGACAGCa                                                                                                       <  1:221369/133‑1 (MQ=255)                       cTGGCTAAAAGCAATATCCGCGGTGCACAGCCAATGACGTTTGTGGCGGATAAAGACAGAGCGGTTGTCGTTTTGCAAACATCGAAAGCGGAAATCATTGGGAATGTCGTGGATGAGACAACTCTGACAGCaa                                                                                                      <  1:183893/133‑1 (MQ=255)                        tGGCTAACAGCAATGTCAGCGATGCACAGCCAATGACGGTTGTGGCGGATAAAGACAGAGCGGTTGTCGTTTTGCAAACATCGAAAGCGGAAATCATTGGGAATGTCGTGGATGAGACAACTCTGACAGCAAc                                                                                                     <  1:499156/133‑1 (MQ=255)                                aGCAATGTCAGCGATGCACAGCCAATGACGTTTGTGGCGGATAAAGACAGAGCGGTTGTCGTTTTGCAAACATCGAAAGCGGAAATCATTGGGAATGTCGTGGATGAGACAACTCTGACAGCAACAGTGAAAg                                                                                             <  1:415245/133‑1 (MQ=255)                                                 acaGCCAATGACGTTTGTGGCGGATAAAGACAGAGCGGTTGTCGTTTTGCAAACATCGAAAGCGGAAATCATTGGGAATGTCGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCg                                                                            <  1:340991/133‑1 (MQ=255)                                                         tGACGTTTGTGGCGGATAAAAACAGAGCGGGTGTCGTTTTTCAAACATCGAAAGCGGAAAACATTGGGAATGTCGTGGATGAGACAACTCTGACAGCAACAATGCAAAATCCGTCGAAACATCCCGTGGC‑ggg                                                                   >  2:232670/1‑133 (MQ=255)                                                             gTTTGTGGCGGATAAAGAAAGAACGGATTTTGTTTTGCCAACAACGAAAGAGGAAAACATTTGGAAAGTTTTGGGTGATAAAACTCTCGCATCAAAAATGAAAAGTCCCGCGGAACATCCGGGTGC‑gggggga                                                               >  2:101697/1‑130 (MQ=255)                                                                     cGGATAAAGACAGAGCGGGTGTCGTTTTGCAAACATCGAAAGCGGAAATCATTGGGAATGTCGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGC‑GGGGGTAACGGTaaa                                                       >  1:375314/1‑133 (MQ=255)                                                                        aTAAAGACAGAGCGGTTGTCGTTTTGCAAACATCGAAAGCGGAAATCATTGGGAATGTCGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGC‑GGGGATAACGGTAAACtt                                                    <  1:31768/133‑1 (MQ=255)                                                                                 gagCGGTTGTCGTTTTGCAAACATCGAAAGCGGAAATCATTGGGAATGTCGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGC‑GGGGATAACGGTAAACTTCACCATGcc                                           >  2:24659/1‑133 (MQ=255)                                                                                 gagCGGTTGTCGTTTTGCAAACATCGAAAGCGGAAATCATTGGGAATGTCGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGC‑GGGGATAACGGTAAACTTCACCATGcc                                           >  2:147104/1‑133 (MQ=255)                                                                                  agCGGTTGTCGTTTTGCAAACATCGAAAGCGGAAATCATTGGGAATGTCGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGC‑GGGGATAACGGTAAACTTCACCATGCca                                          >  2:635955/1‑133 (MQ=255)                                                                                  agCGGTTGTCGTTTTGCAAACATCGAAAGCGGAAATCATTGGGAATGTCGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGC‑GGGGATAACGGTAAACTTCACCATGCca                                          >  2:255994/1‑133 (MQ=255)                                                                                  agCGGTTGTCGTTTTGCAAACATCGAAAGCGGAAATCATTGGGAATGTCGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGC‑GGGGATAACGGTAAACTTCACCATGCca                                          >  1:625173/1‑133 (MQ=255)                                                                                  agCGGTTGTCGTTTTGCAAACATCGAAAGCGGAAATCATTGGGAATGTCGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGC‑GGGGAGAACGGTGAACTTCACCATGCca                                          >  1:196832/1‑133 (MQ=255)                                                                                                                 gAAATCATTGGGAATGTCGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGC‑GGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGaaa           >  2:550872/1‑133 (MQ=255)                                                                                                                   aaTCATTGGGAATGTCGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGC‑GGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAAt         <  1:104288/133‑1 (MQ=255)                                                                                                                    aTCATTGGGACTGTCCTGGATGAGACAAATCTAACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGC‑GGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATa        <  2:196832/133‑1 (MQ=255)                                                                                                                    aTCATTGGGAATGTCTTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGC‑GGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATa        <  1:255994/133‑1 (MQ=255)                                                                                                                         tGGGAATGTCGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACgg    >  1:71185/1‑133 (MQ=255)                                                                                                                          gggAATGTCGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGC‑GGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTa  <  1:416410/133‑1 (MQ=255)                                                                                                                                 |                                                                                                                               CACGCATATGATCATGGCTCGTCTGGCTAACAGCAATGTCAGCGATGCACAGCCAATGACGTTTGTGGCGGATAAAGACAGAGCGGTTGTCGTTTTGCAAACATCGAAAGCGGAAATCATTGGGAATGGCGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGC‑GGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTA  >  NC_000913/2047979‑2048232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑