Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,107,777	G→A	R185C (CGC→TGC)	glf ←	UDP‑galactopyranose mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,107,777	0	G	A	100.0%	142.5 / NA	42	R185C (CGC→TGC)	glf	UDP‑galactopyranose mutase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/24);  total (18/24)

ATGATTCTATGGGCTTTACTCGCTAGAGAATCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGT  >  NC_000913/2107647‑2107905                                                                                                                                   |                                                                                                                                 atgatTCTATGGGCTTTACTCGCTAGAGAATCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCCGAATACCTTAATAGCAAt                                                                                                                                >  1:279087/1‑133 (MQ=255) atgatTCTATGGGCTTTACTCGCTAGAGAATCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAAGAAGCTTAGCGTAGCCTGCCACCGGAATACCATGATAGCAAt                                                                                                                                >  2:167135/1‑133 (MQ=255)      tCTATGGGCTTTACTCGCTAGAGAATCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGTAATTCCGTGATACGAATCGGaa                                                                                                                           >  1:185332/1‑133 (MQ=255)        tATGGGCTTTACTCGCTAGGGAATCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCAATCGGaaaa                                                                                                                         >  1:4635/1‑133 (MQ=255)        tATGGGCTTTACTCGCTAGAGAATCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCACACCGGATTACGTTAATAGCATTCGGaaaa                                                                                                                         >  1:284061/1‑133 (MQ=255)                  aCTCGCTAGAGAATCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCAATCGGAAAAATAATTGTTa                                                                                                               >  1:7131/1‑133 (MQ=255)                   cccGCTAGATGATCTCTGTCTTTCGACATATCGATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCAATCGGAAAAATAATTGTTAt                                                                                                              <  2:284061/131‑1 (MQ=255)                   cTCGCTAGGGAATCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCAATCGGAAAAATAATTGTTAt                                                                                                              <  2:4635/133‑1 (MQ=255)                    tCGCTAGAGAATCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCAATCGGAAAAATAATTGTTATc                                                                                                             <  1:367074/133‑1 (MQ=255)                    tCGCTAGAGAATCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCAATCGGAAAAATAATTGTTATc                                                                                                             <  1:398974/133‑1 (MQ=255)                    tCGCTAGAGAATCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCAATCGGAAAAATAATTGTTATc                                                                                                             <  1:619714/133‑1 (MQ=255)                       cTAGAGAATCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCAATCGGAAAAATAATTGTTATCaaa                                                                                                          >  2:329808/1‑133 (MQ=255)                        tAGAGAATCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCAATCGGAAAAATAATTGTTATCAAAc                                                                                                         <  2:575870/133‑1 (MQ=255)                             aaTCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCAATCGGAAAAATAATTGTTATCAAACGTaaa                                                                                                    <  2:7131/133‑1 (MQ=255)                                    gTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCAATCGGAAAAATAATTGTTATCAAACGTAAATCTCACt                                                                                             >  1:54790/1‑133 (MQ=255)                                       tttCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCAATCGGAAAACTAATTGTAATCAAACGTAAATCTCACTggg                                                                                          >  1:210174/1‑133 (MQ=255)                                          cAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCAATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGAtt                                                                                       >  2:538019/1‑133 (MQ=255)                                               aaTCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCAATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCtt                                                                                  <  1:329808/133‑1 (MQ=255)                                                aTCAATGCCTAATTTTACGTCCACACTTGCAACCATTTTTTAAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCAATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTta                                                                                 <  2:279087/133‑1 (MQ=255)                                                                          cTTCAAGCATTTTTTGAATAAGCTTAGTCTAGACTCCCACCTGAATACCTTGATAGCAATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGc                                                       <  2:210174/133‑1 (MQ=255)                                                                          cTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCAATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGc                                                       <  2:419208/133‑1 (MQ=255)                                                                             cAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCAATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCAct                                                    >  1:330436/1‑133 (MQ=255)                                                                             cAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCAATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCAct                                                    >  1:491253/1‑133 (MQ=255)                                                                              aaGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCAATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCActt                                                   <  1:372840/133‑1 (MQ=255)                                                                                            tAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCAATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCtt                                     <  1:476004/133‑1 (MQ=255)                                                                                              aGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCAATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTtct                                   <  1:588156/133‑1 (MQ=255)                                                                                              aGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCAATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTtct                                   <  2:580991/133‑1 (MQ=255)                                                                                                    gtgtAGCCTCCCACCGGAATACCTTGATAGCAATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGtat                             <  2:491253/133‑1 (MQ=255)                                                                                                    gtgtAGCCTCCCACCGGAATACCTTGATAGCAATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGtat                             <  2:330436/133‑1 (MQ=255)                                                                                                        aGCCTCCCACCGGAATACCTTGATAGCAATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAAcc                         <  2:407228/133‑1 (MQ=255)                                                                                                         gCCTCCCATCGGAATGCCTTGACAGCAATCCGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAAccc                        <  1:308466/133‑1 (MQ=255)                                                                                                         gCCTCCCACCGGAATACCTTGATAGCAATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAAccc                        <  2:88973/133‑1 (MQ=255)                                                                                                          ccTCCCACCGGAATACCTTGATAGCAATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCt                       >  1:499844/1‑133 (MQ=255)                                                                                                          ccTCCCACCGGAATACCTTGATAGCAATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCt                       >  1:475445/1‑133 (MQ=255)                                                                                                          ccTCCCACCGGAATACCTTGATAGCAATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACAGCTTCTCCGCATAACCCt                       >  1:264886/1‑133 (MQ=255)                                                                                                               cACCGGAATACCTTGATAGCAATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATc                  >  2:534685/1‑133 (MQ=255)                                                                                                                   ggAATACCTTGATAGCAATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATg              <  2:54790/133‑1 (MQ=255)                                                                                                                     aaTACCTTGATAGCAATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCt            <  2:238533/133‑1 (MQ=255)                                                                                                                     aaTACCTTGATAGCAATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCt            <  2:499844/133‑1 (MQ=255)                                                                                                                           ttGATAGCAATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGtat      <  1:538019/133‑1 (MQ=255)                                                                                                                            tGATAGCAATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGtata     >  2:424361/1‑133 (MQ=255)                                                                                                                             gATAGCAATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATaa    >  2:105131/1‑133 (MQ=255)                                                                                                                               tAGCAATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATACTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGt  <  2:43067/133‑1 (MQ=255)                                                                                                                                   |                                                                                                                                 ATGATTCTATGGGCTTTACTCGCTAGAGAATCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGT  >  NC_000913/2107647‑2107905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑