Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,479,687	(G)6→5	coding (550/1758 nt)	ynbC →	hydrolase/methyltransferase domain‑containing protein YnbC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,479,682	0	G	.	100.0%	162.6 / NA	39	coding (545/1758 nt)	ynbC	hydrolase/methyltransferase domain‑containing protein YnbC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (23/16);  total (23/16)

TGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATAT  >  NC_000913/1479544‑1479816                                                                                                                                           |                                                                                                                                       tGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTGATTAATTCTTATGTAAGCGGACGCTATTTGAGCCACGATTGGCAACgg                                                                                                                                       >  2:394128/1‑140 (MQ=255)         tctcCGGCCTTTAAGGTTAAATTGTATGTGCTGCTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGt                                                                                                                              <  2:284313/140‑1 (MQ=255)              ggCCTTTAAGGTTAAGTTGTATATGACGCAGGCACGTTCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCaa                                                                                                                         <  1:394128/140‑1 (MQ=255)                ccTTTAAGGTTAAATTGTATGTGCCGCTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCaata                                                                                                                       >  1:132820/1‑140 (MQ=255)                        gTTAAATTGTATGTGCCGCTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCg                                                                                                               <  2:753138/140‑1 (MQ=255)                                      gCCGCTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGc                                                                                                 >  1:120181/1‑140 (MQ=255)                                       ccGCTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCg                                                                                                >  2:571940/1‑140 (MQ=255)                                        cGCTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGa                                                                                               <  1:719728/140‑1 (MQ=255)                                        cGCTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTACAATAACGATCCGTTGATCACACGGGCGa                                                                                               >  1:267903/1‑140 (MQ=255)                                         gCTGGCACGTCCTGCGCTGGCGCTATGGCATAGTCCGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGAt                                                                                              <  2:267903/140‑1 (MQ=255)                                          cTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGAtt                                                                                             <  2:11125/140‑1 (MQ=255)                                           tGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTg                                                                                            >  2:590025/1‑140 (MQ=255)                                                 gTCCTGCGTTGGCGTTACGGCATCGTCTGCGTGGTCTGTTTTTTATTATTTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTa                                                                                      <  2:388711/140‑1 (MQ=255)                                                       cgcTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCt                                                                                >  1:560552/1‑140 (MQ=255)                                                            gCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTc                                                                           >  1:25908/1‑140 (MQ=255)                                                               ttATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGAt                                                                        <  2:120181/140‑1 (MQ=255)                                                                 aTGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGAtct                                                                      >  2:799507/1‑140 (MQ=255)                                                                            tGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACaa                                                                <  2:805250/135‑1 (MQ=255)                                                                            tGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACaa                                                                >  1:805250/1‑135 (MQ=255)                                                                                   tCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGaa                                                      <  2:75964/138‑1 (MQ=255)                                                                                   tCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGaa                                                      >  1:75964/1‑138 (MQ=255)                                                                                   tCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGa                                                       <  2:76240/137‑1 (MQ=255)                                                                                   tCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGa                                                       >  1:76240/1‑137 (MQ=255)                                                                                   tCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACg                                                    >  1:759791/1‑140 (MQ=255)                                                                                   tCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACg                                                    >  2:638809/1‑140 (MQ=255)                                                                                               taATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAgaga                                        >  1:706207/1‑140 (MQ=255)                                                                                                       aTGTGAAAGGACGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgcggcgg                                >  1:646025/1‑140 (MQ=255)                                                                                                        tgtgAAAGGACGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgcggcggc                               >  1:549177/1‑140 (MQ=255)                                                                                                        tgtgAAAGGACGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgcggcggc                               >  2:799233/1‑140 (MQ=255)                                                                                                         gtgAAAGGACGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgcggcggcg                              <  2:549177/140‑1 (MQ=255)                                                                                                              aGGACGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACATAACGTCTCAACGTATTCTTAGAGATGCGGCGGCGATTAc                         >  2:286666/1‑140 (MQ=255)                                                                                                                  cGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTc                     >  2:492685/1‑140 (MQ=255)                                                                                                                  cGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTACAGATGCGGCGGCGATTACGCTc                     >  2:252006/1‑140 (MQ=255)                                                                                                                    cTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTccc                   >  2:755850/1‑140 (MQ=255)                                                                                                                          gACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCa             <  2:759791/140‑1 (MQ=255)                                                                                                                             ccACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGAGGCGACGGCGATTAAGCTCCCCACGCAACt          >  1:349819/1‑140 (MQ=255)                                                                                                                               aCGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTc        >  1:499866/1‑140 (MQ=255)                                                                                                                               aCGATCGGCAAC‑GGGGGCGAGTGTCAATACTGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTc        <  1:286666/140‑1 (MQ=255)                                                                                                                                     ggCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGatat  <  2:646025/140‑1 (MQ=255)                                                                                                                                     ggCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGatat  <  1:799233/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                       TGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATAT  >  NC_000913/1479544‑1479816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑