| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,787,826 | Δ1 bp | coding (382/834 nt) | ppsR → | posphoenolpyruvate synthetase regulatory protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,787,826 | 0 | G | . | 94.4% | 62.1 / ‑2.3 | 18 | coding (382/834 nt) | ppsR | posphoenolpyruvate synthetase regulatory protein |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base . (8/9); total (8/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGT > NC_000913/1787701‑1787945 | aaGGCTTTTGCCAGGATATCGTGCAGGCGATGGTTGCACCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGAT‑CGCGCATTGCGGCga < 1:374813/140‑1 (MQ=255) aGGCTTTTACCAGGATATCGTTAAGGCGCTGATTGACCCTCTACAACAGGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGAT‑CGCGCATTGCGGCgat < 2:373794/140‑1 (MQ=255) aGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGAT‑CGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATg > 1:776652/1‑140 (MQ=255) ggCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGAT‑CGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGa > 1:626701/1‑140 (MQ=255) cTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGAT‑CGCGCATTGCGGCGATTGCTTACACCCTCGCCCACGATGACGGc > 1:304300/1‑140 (MQ=255) tGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGAT‑CGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAgg < 1:525160/140‑1 (MQ=255) gAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGAT‑CGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGc < 2:626701/140‑1 (MQ=255) gAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGAT‑CGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGc < 2:62366/140‑1 (MQ=255) gATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGAT‑CGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTg > 1:551267/1‑140 (MQ=255) aTCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGAT‑CGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGATGGCATTTAGTTGCGAAATCTGAACCAGGCTCAGGTGa > 1:234225/1‑140 (MQ=255) aTTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGAT‑CGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAgg > 1:455919/1‑126 (MQ=255) aTTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGAT‑CGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAgg < 2:455919/126‑1 (MQ=255) tACCCATGGCCTTAACCCTAATAATCTCAATAAATATGAT‑CGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCtg < 2:776652/140‑1 (MQ=255) tACCCATGGCCTTAACCCTAATAATCTCAATAAATATGA‑GCGCGCATTTCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCtg < 2:304300/140‑1 (MQ=255) aaCCCTAATAATCTCAATAAATATGAT‑CGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAAccccc > 1:194716/1‑140 (MQ=255) cccTAATAATCTCAATAAATATGAT‑CGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCccac < 2:551267/140‑1 (MQ=255) cccTAATAATCTCAATAAATATGAT‑CGCGCACTGCTGCGATTGATTACTCCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCccac < 2:234225/140‑1 (MQ=255) aataatCTCAATAAATATGAT‑CGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGt > 1:99598/1‑140 (MQ=255) | AAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGT > NC_000913/1787701‑1787945 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |