Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,803,347	(T)7→6	coding (2000/2145 nt)	nrdE →	ribonucleoside‑diphosphate reductase 2 subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,803,341	0	T	.	100.0%	268.8 / NA	64	coding (1994/2145 nt)	nrdE	ribonucleoside‑diphosphate reductase 2 subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (38/26);  total (38/26)

GTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGCTTTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCTGCGTCAGATGGCGCTGGAAGGCACTGAAATTGAAGGCTGC  >  NC_000913/2803205‑2803474                                                                                                                                         |                                                                                                                                      gTTTACTACCCTGCCCCTTTTAAGACTAACGCGAATCTGTCGCTGTATAAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGCtttt                                                                                                                                    <  2:240351/140‑1 (MQ=255)          ccTGCCCCGTTTATGTCTAACGAGATTCTCGCGCTGTGTCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGAt                                                                                                                          <  1:277202/140‑1 (MQ=255)           cTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGACGATCAGAGGCCGTCGCTGACGC‑TTTTTTCCCCGATa                                                                                                                         >  1:328076/1‑140 (MQ=255)             gCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATAcc                                                                                                                       >  2:22286/1‑140 (MQ=255)                  gTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGccac                                                                                                                  <  2:524693/140‑1 (MQ=255)                         aCTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTcgc                                                                                                           <  1:757206/140‑1 (MQ=255)                             aCGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGata                                                                                                       >  2:85856/1‑140 (MQ=255)                             aCGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGata                                                                                                       >  2:813993/1‑140 (MQ=255)                              cGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGatat                                                                                                      >  1:26559/1‑140 (MQ=255)                              cGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTCACGC‑TTTGTTCGCCTATACCGCCACCACTCGCGatat                                                                                                      >  1:255953/1‑140 (MQ=255)                                   aTCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATcaaca                                                                                                 <  1:85856/140‑1 (MQ=255)                                    tCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATcaacaa                                                                                                >  1:525952/1‑140 (MQ=255)                                       ggCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAgc                                                                                             >  2:147005/1‑140 (MQ=255)                                       ggCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAgc                                                                                             >  1:48722/1‑140 (MQ=255)                                        gcgcTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAgcg                                                                                            <  1:22286/140‑1 (MQ=255)                                        gcgcTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAgcg                                                                                            <  2:622221/140‑1 (MQ=255)                                        gcgcTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAgcg                                                                                            <  2:83483/140‑1 (MQ=255)                                            tGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGa                                                                                        >  2:594745/1‑140 (MQ=255)                                                    gACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGcc                                                                                <  1:551203/140‑1 (MQ=255)                                                       gCTTACGCAATTGGCGCCGAAAAGATCATCGTCACCTACCCGGAAGCGTCTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTgg                                                                             <  2:227081/140‑1 (MQ=255)                                                        cTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACTACTCGCGCTATGAATAAAGCGTAGATTTACGCCTGgc                                                                            >  1:389151/1‑140 (MQ=255)                                                        cTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGgc                                                                            >  2:765122/1‑140 (MQ=255)                                                        cTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGgc                                                                            >  2:634626/1‑140 (MQ=255)                                                        cTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGgc                                                                            >  2:556953/1‑140 (MQ=255)                                                        cTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGgc                                                                            >  1:783615/1‑140 (MQ=255)                                                        cTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGgc                                                                            >  1:130040/1‑140 (MQ=255)                                                        cTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGgc                                                                            >  1:689860/1‑140 (MQ=255)                                                        cTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGgc                                                                            >  1:683670/1‑140 (MQ=255)                                                        cTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGgc                                                                            >  1:651541/1‑140 (MQ=255)                                                        cTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGgc                                                                            >  1:644166/1‑140 (MQ=255)                                                        cTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGgc                                                                            >  1:27811/1‑140 (MQ=255)                                                        cTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGgc                                                                            >  1:578011/1‑140 (MQ=255)                                                        cTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACAACTCGCGTTATAAAGAAAGCGAAGATTTACGCCTGgc                                                                            >  1:227081/1‑140 (MQ=255)                                                          tACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGgcgc                                                                          <  1:16640/140‑1 (MQ=255)                                                          tACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGgcgc                                                                          <  2:783615/140‑1 (MQ=255)                                                          tACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGgcgc                                                                          >  1:28575/1‑140 (MQ=255)                                                               aaTTGGCGCAGAAAAGATCATCTACACCTACCCGGTAGCAACTCGCCAAGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAggg                                                                     <  2:255953/140‑1 (MQ=255)                                                                     cgcAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCaa                                                               >  1:147367/1‑140 (MQ=255)                                                                       cAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCaaaa                                                             <  2:525952/140‑1 (MQ=255)                                                                       cAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCaaaa                                                             <  1:594745/140‑1 (MQ=255)                                                                       cAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCaaaa                                                             <  2:48722/140‑1 (MQ=255)                                                                        aGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGCATCAAAAc                                                            <  1:147005/140‑1 (MQ=255)                                                                         gAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACg                                                           <  2:161806/140‑1 (MQ=255)                                                                          aaaaGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGc                                                          <  1:556953/140‑1 (MQ=255)                                                                             aGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGctct                                                       <  1:813993/140‑1 (MQ=255)                                                                                  atcGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTAc                                                  <  1:634626/140‑1 (MQ=255)                                                                                    cGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACAt                                                >  1:831136/1‑140 (MQ=255)                                                                                    cGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACAt                                                >  1:665772/1‑140 (MQ=255)                                                                                         ccTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGcc                                           >  2:578400/1‑140 (MQ=255)                                                                                          cTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCt                                          <  2:26559/140‑1 (MQ=255)                                                                                          cTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCCGGCGCAATGGTGTCTAAACGCGCTATTACATCCGCCt                                          >  1:383078/1‑140 (MQ=255)                                                                                                  aaGCGACTCGCCATGTTGATCAGGGGCTATGGCCGTCGC‑TTTTTTCCCTGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCTGCGTCAGa                                  <  2:389151/140‑1 (MQ=255)                                                                                                    gCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCTGCGTCAGATg                                >  2:587206/1‑140 (MQ=255)                                                                                                            ccATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCTGCGTCAGATGGCGCTGGa                        >  2:12081/1‑140 (MQ=255)                                                                                                            ccATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGAATCAAAACGCTCTATTACATCCGCCTGCGTCAGATGGCGCTGGa                        >  1:98683/1‑140 (MQ=255)                                                                                                                 tCGATCAGGGGCTGTCGTTGACGC‑TTTTTTCTCCGTTACGGCCACCACACGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCTGCGTCAGATGGCGCTGGAAGGCa                   <  2:322620/140‑1 (MQ=255)                                                                                                                    aTCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCTGCGTCAGATGGCGCTGGAAGGCACTg                <  2:27811/140‑1 (MQ=255)                                                                                                                         gggCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCTGGGTCGGATGGCGCTGGAAGGCACTGAAAtt           >  1:279914/1‑140 (MQ=255)                                                                                                                           gCTGTCGCTGACGC‑TTTTTTCCCCGATACAGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCCAGGGTATCAAAACGCTCTATTACATCCGCCTGCGTCAGATGGCGCTGGAAGGCACTGAAATTGa         >  2:63402/1‑140 (MQ=255)                                                                                                                            cTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCTGCGTCAGATGGCGCTGGAAGGCACTGAAATTGaa        <  2:28575/140‑1 (MQ=255)                                                                                                                              gTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCTGCGTCAGATGGCGCTGGAAGGCACTGAAATTGAAgg      >  1:173849/1‑140 (MQ=255)                                                                                                                              gTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCTGCGTCAGATGGCGCTGGAAGGCACTGAAATTGAAgg      >  1:100496/1‑140 (MQ=255)                                                                                                                               tCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCTGCGTCAGCTGGGGCAGGAAGGCACTGAAATTGAAGGc     >  1:350585/1‑140 (MQ=255)                                                                                                                               tCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCTGCGTCAGATGGCGCTGGAAGGCACTGAAATTGAAGGc     <  2:831136/140‑1 (MQ=255)                                                                                                                               tCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCTGCGTCAGATGGCGCTGGAAGGCACTGAAATTGAAGGc     >  2:192744/1‑140 (MQ=255)                                                                                                                                cGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCTGCGTCAGATGGCGCTGGAAGGCACTGAAATTGAAGGCt    <  2:665772/140‑1 (MQ=255)                                                                                                                                  cTGACGC‑TTTTTTCCCCGATACCGCCCCCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCTGCGTCAGATGGCGCTGGAAGGCACTGAAATTGAAGGCTGc  <  1:201537/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      GTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGCTTTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCTGCGTCAGATGGCGCTGGAAGGCACTGAAATTGAAGGCTGC  >  NC_000913/2803205‑2803474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑