Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,099,460 (T)5→4 coding (106/1008 nt) yggM ← DUF1202 domain‑containing protein YggM

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,099,4560T.100.0% 186.8 / NA 45coding (110/1008 nt)yggMDUF1202 domain‑containing protein YggM
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (26/19);  total (26/19)

CCAGGTATAGAGATCGTCACTGGAAGAGACATCGCCTTCCGCTGACCAGGTGGCCTGATTACCCTTAGCATCAAGATTTTTTAAGGTGATAGCATCAAGCTTGAGAATGCCGTGATACTGCTTTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGTTCGCCATCTGCCCAGGCATTACCAGTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAAT  >  NC_000913/3099335‑3099589
                                                                                                                         |                                                                                                                                     
ccAGGTATAGAGATCGTCACTGGAAGAGACATCGCCTTCCGCTGACCAGGTGGCCTGATTACCCTTAGCATCAAGATTTTTTAAGGTGATACCATCAAGCTTGAAACTGCCGTGATAGTGC‑TATTGAATTGTTCTTTTaa                                                                                                                    >  2:389519/1‑140 (MQ=255)
             tCGTCACTGGAAGAGACATCGCCTTCCGCTGACCAGGTGGCCTGATTACCCTTAGCATCAAGATTTTTTAAGGTGATAGCATCAAGCTTGAGAATGCCGTGAAACTGC‑TTATGAGTTGATCTTTGAAGATATTTTCAGtt                                                                                                       >  1:212436/1‑140 (MQ=255)
              cGTCACTGGAAGAGACATCGCCTTCCGCTGACCAGGTGGCCTGATTACCCTTAGCATCAAGATTTTTTAAGGTGATAGCATCAAGCTTGAGTATGCCGTGGTACTGC‑TTTTGAATTGGTCTTGAAAGATATTTTCAGTTg                                                                                                      >  2:401065/1‑140 (MQ=255)
              cGTCACTGGAAGAGACATCGCCTTCCGCTGACCAGGTGGCCTGATTACCCTTAGCATCAAGATTTTTTAAGGTGATAGCATCAAGCTTGAGAATGCCGTGATACTGC‑TTTTGAATTGATCTTTTGAGATATTTTCAGTTg                                                                                                      >  1:335123/1‑140 (MQ=255)
              cGTCACTGGAAGAGACATCGCCTTCCGCTGACCAGGTGGCCTGATTACCCTTAGCATCAAGATTTTTTAAGGTGATAGCATCAAGCTTGAGAATGCCGTGATACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTg                                                                                                      >  2:455291/1‑140 (MQ=255)
              cGTCACTGGAAGAGACATCGCCTTCCGCTGACCAGGTGGCCTGATTACCCTTAGCATCAAGATTTTTTAAGGTGATAGCATCAAGCTTGAGAATGCCGTGATACTGC‑TTTTGAATTGATCTTTTAAGATA‑TTTCAGTTgg                                                                                                     >  1:791585/1‑140 (MQ=255)
              cGTCACTGGAAGAGACATCGCCTTCCGCTGACCAGGTGGCCTGATTACCCTTAGCATCAAGATTTTTTAAGGTGATAGCATCAAGCTTGAGAATGCCGTGATACTGC‑TTTGGAAATGCTCTTTTAAGATATTTTCAGTTg                                                                                                      >  1:287730/1‑140 (MQ=255)
               gTCACTGGAAGAGACTTCGCCTTCCCCTGTCCAGGTGACCTGATTACCCTTAGCATCAAGATTTTTTAAGGTGATAGCATCAAGCTTGAGAATGCCGTGATACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTgg                                                                                                     <  1:389519/140‑1 (MQ=255)
                            aCATCGCCTTCCGCTGACCAGGTGGCCTGATTACCCTTAGCATCAAGATTTTTTAAGGTGATAGCATCAAGCTTGAGAATGCCGTGATACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGTTCGCCATCt                                                                                        >  2:113184/1‑140 (MQ=255)
                              aTCGCCTTCCGCTGACCAGGTGGCCTGATTACCCTTAGCATCAAGATTTTTTAAGGTGATAGCATCAAGCTTGAGAATGCCGTGATACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGTTCGCCATCTGc                                                                                      >  2:621232/1‑140 (MQ=255)
                              aTCGCCTTCCGCTGACCAGGTGGCCTGATTACCCTTAGCATCAAGATTTTTTAAGGTGATAGCATCAAGCTTGAGAATGCCGTGATACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGTTCGCCATCTGc                                                                                      >  2:173743/1‑140 (MQ=255)
                              aTCGCCTTCCGCTGACCAGGTGGCCTGATTACCCTTAGCATCAAGATTTTTTAAGGTGATAGCATCAAGCTTGAGAATGCCGTGATACTGC‑CTTTGAATTTATCGTTTTAGATATTTTCAGTTTGCGGTTCGCCATCTGc                                                                                      >  2:386212/1‑140 (MQ=255)
                               tCGCCTTCCGCTGACCAGGTGGCCTGATTACCCTTAGCATCAAGATTTTTTAAGGTGATAGCATCAAGCTTGAGAATGCCGTGATACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGTTCGCCATCTGcc                                                                                     <  1:750204/140‑1 (MQ=255)
                               tCGCCTTCCGCTGACCACGTGGCCTGATTACTCTTCGCATCAAGATTTATTAAGGTGATAGCATCAAGCTTGAGAATGCCGTGATTCTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGTTCGCCATCTGcc                                                                                     <  2:287730/140‑1 (MQ=255)
                                cGCCTTCCGCTGACCAGGTGGCCTGATTACCCTTAGCATCAAGATTTTTTAAGGTGATAGCATCAAGCTTGAGAATGCCGTGATACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCTGTTGGCGGTTCGCCATCTGccc                                                                                    >  1:331858/1‑140 (MQ=255)
                                 gCCTTCCGCTGACCACGTGGCCGGAATACCCTTAGCATCAAGATTTTTTAAGGTGATAGCATCAAGCTTGAGAATGCCGTGATACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGTTCGCCATCTGCCCa                                                                                   <  1:401065/140‑1 (MQ=255)
                                             ccAGGTGGCCTGATTACCCTTAGCATCAAGATTTTTTAAGGTGATAGCATCAAGCTTGAGAATGCCGTGATACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGTTCGCCATCTGCCCAGGCATTACCAGt                                                                       >  2:436564/1‑140 (MQ=255)
                                               aGGTGGCCTGATTACCCTTAGCATCAAGATTTTTTAAGGTGATAGCATCAAGCTTGAGAATGCCGTGATACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGTTCGCCATCTGCCCAGGCATTACCAGtca                                                                     <  2:564284/140‑1 (MQ=255)
                                               aGGTGGCCTGATTACCCTTAGCATCAAGATTTTTTAAGGTGATAGCATCAAGCTTGAGAATGCCGTGATACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGTTCGCCATCTGCCCAGGCATTACCAGtca                                                                     <  1:621232/140‑1 (MQ=255)
                                               aGGTGGCCTGATTACCCTTAGCATCAAGATTTTTTAAGGTGATAGCATCAAGCTTGAGAATGCCGTGATACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGTTCGCCATCTGCCCAGGCATTACCAGtca                                                                     <  2:650360/140‑1 (MQ=255)
                                               aGGTGGCCTGATTACCCTTAGCATCAAGATTTTTTAAGGTGATAGCATCAAGCTTGAGAATGCCGTGATACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGTTCGCCATCTGCCCAGGCATTACCAGtca                                                                     <  1:820405/140‑1 (MQ=255)
                                                 gTGGCCTGATTACCCTTAGCATCAATATTGTTTAAGGTGATAGCATCAAGCTTGAGAATGCCGTGATACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGTTCGCCATCTGCCCAGGCATTACCAGtcatc                                                                   <  1:286102/140‑1 (MQ=255)
                                                   ggCCTGATTACCCTTAGCATCAAGATTTTTTAAGGTGATAGCATCAAGCTTGAGAATGCCGTGATACTGC‑TTTTGAATTGATCTTTTAAGATA‑TTTCAGTTGGCGGTTCGCCATCTGCCCAGGCATTACCAGTCATCAAc                                                                <  2:791585/140‑1 (MQ=255)
                                                      cTGATTACCCTTAGCATCAAGATTTTTTAAGGTGATAGCATCAAGCTTGAGAATGCCGTGATACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGTTCGCCATCTGCCCAGGCATTACCAGTCATCAACAt                                                              >  1:608677/1‑140 (MQ=255)
                                                      cTGATTACCCTTAGCATCAAGATTTTTTAAGGTGATAGCATCAAGCTTGAGAATGCCGTGATACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGTTCGCCATCTGCCCAGGCATTACCAGTCATCAACAt                                                              <  1:455291/140‑1 (MQ=255)
                                                           tACCCTTAGCATCAAGATTTTTTAAGGTGATAGCATCAAGCTTGAGAATGCCGTGATACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGTTCGCCATCTGCCCAGGCATTACCAGTCATCAACATAAGCa                                                         >  2:204279/1‑140 (MQ=255)
                                                             cccTTAGCATCAAGAGTTTTTAAGGTGATGGCATCAAACTTGAGAATGCCGTGATACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGTTCGCCATCTGCCCAGGCATTACCAGTCATCAACATAAGCAgt                                                       <  1:313097/140‑1 (MQ=255)
                                                                   gCATCAAGATTTTTTAAGGTGATAGCATCAAGCTTGAGAATGCCGTGATACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGTTCGCCATCTGCCCAGGCATTACCAGTCATCAAc                                                                <  2:158367/125‑1 (MQ=255)
                                                                   gCATCAAGATTTTTTAAGGTGATAGCATCAAGCTTGAGAATGCCGTGATACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGTTCGCCATCTGCCCAGGCATTACCAGTCATCAAc                                                                >  1:158367/1‑125 (MQ=255)
                                                                   gCATCAAGATTTTTTAAGGTGATAGCATCAAGCTTGAGAATGCCGTGATACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGTTCGCCATCTGCCCAGGCATTACCAGTCATCAACATAAGCAgtgccgtg                                                 >  1:689073/1‑140 (MQ=255)
                                                                   gCATCAAGATTTTTTAAGGTGATAGCATCAAGCTTGAGAATGCCGTGATACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGGTCGCCATCAGCCCAGGCATTACCAGACATCAACATAAGCAgtgccgtg                                                 >  2:271495/1‑140 (MQ=255)
                                                                   gCATCAAGATTTTTTAAGGTGATAGCATCAAGCTTGAGAATGCCGTGATACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGATCGCCATCTGCCAAGGAATTACCAGACATCCACATAAGCAgtgccgtg                                                 >  2:313097/1‑140 (MQ=255)
                                                                   gCATCAAGATTTTTTAAGGGGATAGCATCAAGCTTGCGAATGCCGTGATAATGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTGGGCGGTTCGCCATCTGCCCAGGCATTACCAGTCATCAACATAATCAgtgccgtg                                                 >  2:692996/1‑140 (MQ=255)
                                                                       cAAGATTTTTTAAGGTGATAGCATCAAGCTTGAGAATGCCGTGATACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGTTCGCCATCTGCCCAGGCATTACCAGTCATCAACATAAGCAGTGCCGTGCcaa                                             <  1:761161/140‑1 (MQ=255)
                                                                                          aGCATCAAGCTTGAGAACGCCGCGATACTGC‑CTTTCAATCGATCTTTTTAGATATTTTCAGTTGGCGGTTCGCCATCTGCCCAGGCATTACCAGTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCAc                          <  2:212436/140‑1 (MQ=255)
                                                                                               cAAGCTTGAGAATGCCGTGATACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGTTCGCCATCTGCCCAGGCATTACCAGTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGaa                     >  1:521463/1‑140 (MQ=255)
                                                                                                      gagaATGCCGTGATACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGTTCGCCATCTGCCCAGGCATTACCAGTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTc              >  1:660115/1‑140 (MQ=255)
                                                                                                      gagaATGCCGTGATACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGTTCGCCATCTGCCCAGGCATTACCAGTCATCAACAAAAGCAGTGCCGTGCCAGCAATCCATTGCTTGTTCACCCGAAAAACCTc              >  2:242585/1‑140 (MQ=255)
                                                                                                       agaATGCCGTGATACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGTTCGCCATCTGCCCAGGCATTACCAGTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCa             <  2:521463/140‑1 (MQ=255)
                                                                                                         aaTGCCGTGATACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGTTCGCCATCTGCCCAGGCATTACCAGTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCAcg           <  1:204279/140‑1 (MQ=255)
                                                                                                         aaTGCCGTGATACTGC‑ATTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGTTCGTCATCTGCCCAGGCATTACCAGTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCAcg           <  2:498872/140‑1 (MQ=255)
                                                                                                         aaTGCCGTGATACTG‑ATTTTGACTTCATCGTTTAAGAAATTTTCAGTTGGCGGTTCGCCATCTGCCCAGGCATTACCAGTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCAcg           <  1:386212/140‑1 (MQ=255)
                                                                                                             ccGTGATACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGTTCGCCATCTGCCCAGGCATTACCAGTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGTAAAACCTCACGCGGa       >  1:332289/1‑140 (MQ=255)
                                                                                                             ccGTGATACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGTTCGCCATCTGCCCAGGCATTACCAGTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGa       <  2:6214/140‑1 (MQ=255)
                                                                                                                tGATACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGTTCGCCATCTGCCCAGGCATTACCAGTCATCAACATAAGCAGTGCGGTGCCAACAATCCATTGCTTTTTCAGCCGAGAAACCTCACGCGGaata    >  2:225907/1‑140 (MQ=255)
                                                                                                                  aTACTGC‑TTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGTTCGCCATCTGCCCAGGCATTACCAGTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGaataat  >  2:50915/1‑140 (MQ=255)
                                                                                                                         |                                                                                                                                     
CCAGGTATAGAGATCGTCACTGGAAGAGACATCGCCTTCCGCTGACCAGGTGGCCTGATTACCCTTAGCATCAAGATTTTTTAAGGTGATAGCATCAAGCTTGAGAATGCCGTGATACTGCTTTTTGAATTGATCTTTTAAGATATTTTCAGTTGGCGGTTCGCCATCTGCCCAGGCATTACCAGTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAAT  >  NC_000913/3099335‑3099589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑