| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,328,195 | (T)5→4 | intergenic (+112/+44) | yhbY → / ← greA | ribosome assembly factor YhbY/transcription elongation factor GreA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,328,191 | 0 | T | . | 96.4% | 105.3 / ‑2.4 | 28 | intergenic (+108/+48) | yhbY/greA | ribosome assembly factor YhbY/transcription elongation factor GreA |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base . (12/15); total (12/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTG > NC_000913/3328060‑3328320 | cGTAAAATCTCGCTGCCACGCTAAGATTATCCTGAAGTTTCACACATTGGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCC‑TTTTCcttt < 2:349038/140‑1 (MQ=255) aaaaTCTCGCTGCCACGCTAAGGTTATCCTAAAGTTAAACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCGGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCC‑TTTTCctttctt < 1:325236/140‑1 (MQ=255) gCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCC‑TTTTCCTTTCTTTACAATACATc < 2:624357/140‑1 (MQ=255) aaGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCC‑TTTTCCTTTCTTTACAATACATCAACATCtt < 1:90668/140‑1 (MQ=255) aTTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCC‑TTTTCCTTTCTTTACAATACATCAACATCTTGAg > 2:467999/1‑140 (MQ=255) tATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCC‑TTTTCCTTTCTTTACAATACATCAACATCTTGAGTa < 2:664133/140‑1 (MQ=255) tATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCC‑TTTTCCTTTCTTTACAATACATCAACATCTTGAGTa < 1:467999/140‑1 (MQ=255) tCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCC‑TTTTCCTTTCTTTACAATACATCAACATCTTGAGTAtt > 2:771147/1‑140 (MQ=255) aGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCC‑TTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTaa > 1:613634/1‑140 (MQ=255) cacaTTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCC‑TTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTAc < 1:771147/140‑1 (MQ=255) gCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCC‑TTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTAtt < 2:197164/140‑1 (MQ=255) tgtAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCC‑TTTTCCTTTCTTTCCAATACATTAACATGTCGAGTATTGTGTAACTCTTACAGGTATTCCAc > 2:361580/1‑140 (MQ=255) tgtAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCC‑TTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTcc < 1:414385/138‑1 (MQ=255) tgtAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCC‑TTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTcc > 2:414385/1‑138 (MQ=255) tAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCC‑TTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTAt < 2:598745/133‑1 (MQ=255) tAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCC‑TTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTAt > 1:598745/1‑133 (MQ=255) tAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCC‑TTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCt > 1:507323/1‑140 (MQ=255) ggggTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCC‑TTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTAtt > 1:51158/1‑126 (MQ=255) ggggTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCC‑TTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTAtt < 2:51158/126‑1 (MQ=255) gcaggAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCC‑TTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTc > 1:456336/1‑140 (MQ=255) gcaggAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCC‑TTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTc > 2:539471/1‑140 (MQ=255) aaaaTGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCC‑TTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGc < 2:613634/140‑1 (MQ=255) aaaTGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCC‑TTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCg < 1:61041/140‑1 (MQ=255) aaTGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCC‑TTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTGCAGGTATTGCACGTTAGTTACTTCATATTCTACTTCGCCGCCCGGCGt > 2:222835/1‑140 (MQ=255) aCGCTCTATTCGTTGATAAAAGGCCGCATAGCGGCC‑TTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTtgat < 2:456336/140‑1 (MQ=255) ctctGTTCGTTGATAAAAGGCCGCATAGCGGCC‑TTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGAc < 1:539471/140‑1 (MQ=255) cGTTGATAAAAGGCCACATAGCCG‑CTTTTCCCTTTCTCTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCacaaca < 1:222835/140‑1 (MQ=255) gCATAGCGGCC‑TTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTg > 2:586190/1‑140 (MQ=255) | CGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTG > NC_000913/3328060‑3328320 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |