Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,712,926	(T)8→7	coding (9/699 nt)	yhjY ←	conserved protein YhjY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,712,919	0	T	.	98.5%	273.2 / ‑2.9	67	coding (16/699 nt)	yhjY	conserved protein YhjY
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base . (29/37);  minor base A (1/0);  total (30/37)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATCCCAGGTGTAACGCTCTGTGGAAAGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGA  >  NC_000913/3712781‑3713046                                                                                                                                           |                                                                                                                                tATCCCAGGTGTAACGCTCTGTGGAAAGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGCtt                                                                                                                                <  2:822799/140‑1 (MQ=255)   tCCCAGGTGTAACGCTCTGTGGAAAGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGCtttt                                                                                                                              >  1:117103/1‑140 (MQ=255)      cAGGTGTAACGCTCTGTGGAAAGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCACCAGCAGTCTTAGCAGCCAGCTACCACCGCttttttt                                                                                                                           >  1:257705/1‑140 (MQ=255)         gtgtAACGCTCTGTGGAAAGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGCtttttttatg                                                                                                                        <  1:157145/140‑4 (MQ=255)           gtAACGCTCTGTGGAAAGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGCtttttttatg                                                                                                                        >  2:525932/1‑135 (MQ=255)           gtAACGCTCTGTGGAAAGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGCtttttttatg                                                                                                                        <  1:525932/138‑4 (MQ=255)             aaCGCTCTGTGGAAAGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCa                                                                                                                   >  1:61175/1‑140 (MQ=255)             aaCGCTCTGTGGAAAGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCa                                                                                                                   >  2:170834/1‑140 (MQ=255)              aCGCTCTGTGGAAAGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCAt                                                                                                                  >  1:609213/1‑140 (MQ=255)              aCGCTCTGTGGAAAGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGACTTAGCTGCCAGCGGCCACCG‑ATTTTTTTATGATCAt                                                                                                                  >  1:215246/1‑140 (MQ=255)                   ctGTGGAAAGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAaca                                                                                                             <  2:61175/140‑1 (MQ=255)                    tgtgGAAAGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGCttttttta                                                                                                                          <  1:68179/127‑2 (MQ=255)                    tgtgGAAAGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGCttttttta                                                                                                                          >  2:68179/1‑126 (MQ=255)                    tgtgGAAAGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCAtt                                                                                                                 <  1:409759/135‑1 (MQ=255)                    tgtgGAAAGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCAtt                                                                                                                 >  2:409759/1‑135 (MQ=255)                    tgtgGAAAGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAacac                                                                                                            <  1:602005/140‑1 (MQ=255)                    tgtgGAAAGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAacac                                                                                                            <  2:768052/140‑1 (MQ=255)                    tgtgGAAAGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAacac                                                                                                            <  1:358201/140‑1 (MQ=255)                    tgtgGAAAGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAacac                                                                                                            <  1:424001/140‑1 (MQ=255)                    tgtgGAAAGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAacac                                                                                                            >  1:703920/1‑140 (MQ=255)                    tgtgGAAAGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAacac                                                                                                            <  1:17340/140‑1 (MQ=255)                      tgGAAAGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGa                                                                                                          <  2:156136/140‑1 (MQ=255)                           aGTCCGGGTTGCGTATCTACGACATATTCTTTTTGGCAGACGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCa                                                                                                     <  2:231783/140‑1 (MQ=255)                           aGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCa                                                                                                     >  2:756646/1‑140 (MQ=255)                              ccGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAggg                                                                                                  >  2:62422/1‑140 (MQ=255)                                gggTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCAtt                                                                                                                 >  2:170738/1‑123 (MQ=255)                                gggTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCAtt                                                                                                                 <  1:170738/123‑1 (MQ=255)                                   ttGCGTATCAACGATATATTCTCGTTGCCAGCCGTCAGCTGTGTTGAGCAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAagag                                                                                             <  2:215246/140‑1 (MQ=255)                                       gTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGa                                                                                         >  1:133300/1‑140 (MQ=255)                                        tATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGCCCACCGC‑TATTTCTATAATCATTAACACGAATCCAGGGAAGAGCCGaa                                                                                        >  2:318903/1‑140 (MQ=255)                                             aCGATATATTCTTGTTGGCAGGTGTAAGCTGTGTTAAGAGAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCgg                                                                                   <  2:338729/140‑1 (MQ=255)                                             aCGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGATAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCgg                                                                                   <  2:330548/140‑1 (MQ=255)                                             aCGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTGCAGGGAAGAGCCGAATTCgg                                                                                   >  2:777296/1‑140 (MQ=255)                                              cGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCGAGCGACCTCCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGc                                                                                  <  1:62422/140‑1 (MQ=255)                                               gATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCa                                                                                 <  2:117103/140‑1 (MQ=255)                                                   tatTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTATTTATGATAATTAACAGGACTCCAGCGAAGAGCCGAATTCGGCAatat                                                                             >  2:247632/1‑140 (MQ=255)                                                        ttgttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTg                                                                        >  2:808717/1‑140 (MQ=255)                                                         tgttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGt                                                                       <  1:553490/140‑1 (MQ=255)                                                          gttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAG‑GCCGAATTCGGCAATATTATTGTCa                                                                     >  1:825256/1‑140 (MQ=255)                                                          gttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATCACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTc                                                                      >  1:625499/1‑140 (MQ=255)                                                           ttgCCAGGCGTAAGCTGTGTTGCGAAAAAAGTCACCGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCa                                                                     <  2:257705/140‑1 (MQ=255)                                                           ttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCa                                                                     >  1:5140/1‑140 (MQ=255)                                                           ttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCa                                                                     >  2:592255/1‑140 (MQ=255)                                                             gCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCAtt                                                                   >  2:619657/1‑140 (MQ=255)                                                               cAGGCGTAAGCTGTGTTGAGAAAAAAGGCACGGATTACCCCGCTCGCCGGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGt                                                                 <  2:317082/140‑1 (MQ=255)                                                                  gCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGTGAAGAGCGGAATTCGGCAATATTATTGTCATTGTATg                                                              >  1:300485/1‑140 (MQ=255)                                                                      aaGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAgg                                                          <  2:133300/140‑1 (MQ=255)                                                                         cTGTGTTGAGAAAAAAGGCACTTATTACCACTCTCCCCATCAGGCTTATCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGata                                                       <  2:315162/140‑1 (MQ=255)                                                                         cTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGata                                                       <  1:738666/140‑1 (MQ=255)                                                                         cTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGata                                                       <  2:609213/140‑1 (MQ=255)                                                                           gtgtTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                     <  1:170834/140‑1 (MQ=255)                                                                            tgttgtGAAAAAGGGACCTGATTACCACGCTCGTCAGCAGACATAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGTAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCg                                                    >  2:520529/1‑140 (MQ=255)                                                                            tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCg                                                    >  1:721648/1‑140 (MQ=255)                                                                             gtTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTAt                                                               >  2:66520/1‑128 (MQ=255)                                                                             gtTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTAt                                                               <  1:66520/128‑1 (MQ=255)                                                                             gtTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAG‑GCCGAATTCGGCAATATTATTGTCATTGTACGAAGGATATCggg                                                  <  2:825256/140‑1 (MQ=255)                                                                              ttGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                  <  2:5140/140‑1 (MQ=255)                                                                              ttGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                  <  1:4998/140‑1 (MQ=255)                                                                              ttGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                  <  1:619657/140‑1 (MQ=255)                                                                              ttGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                  <  1:592255/140‑1 (MQ=255)                                                                              ttGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                  <  1:756646/140‑1 (MQ=255)                                                                                   aaaaaaGGCACTGATAACCACGCTCACCACCAGGCTTGGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtag                                             <  1:318903/140‑1 (MQ=255)                                                                                      aaaGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtagtag                                          >  2:62213/1‑140 (MQ=255)                                                                                      aaaGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAAGATTATTGGCATAGTTTGAAGGGTATCGGGCAtagtag                                          >  1:243780/1‑140 (MQ=255)                                                                                        aGGCACTGATTACCACGCTCGCTAGGAGGCTTAGCTGACAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                        <  1:308605/140‑1 (MQ=255)                                                                                        aGGCACTGATTACCACGCTCGCCAGTAGGTTTAGCTGTCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                        <  1:301084/140‑1 (MQ=255)                                                                                        aGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGTCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                        <  1:284020/140‑1 (MQ=255)                                                                                        aGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                        <  1:62213/140‑1 (MQ=255)                                                                                        aGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                        <  2:422248/140‑1 (MQ=255)                                                                                                  tACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTaaa                              >  1:70150/1‑140 (MQ=255)                                                                                                     cACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                        <  2:731146/127‑1 (MQ=255)                                                                                                     cACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                        >  1:731146/1‑127 (MQ=255)                                                                                                        gCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGa                        >  2:712536/1‑140 (MQ=255)                                                                                                          tCGCCAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACt                      >  1:590949/1‑140 (MQ=255)                                                                                                                        aGCTGCCAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCg        >  1:178257/1‑140 (MQ=255)                                                                                                                              cAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTACATAATGTCTTTTTAACCGATGCGTCAAGa  >  1:279267/1‑140 (MQ=255)                                                                                                                              cAGCGACCACCGC‑TTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGa  >  1:694383/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                TATCCCAGGTGTAACGCTCTGTGGAAAGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCCAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGA  >  NC_000913/3712781‑3713046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑