| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,850,448 | (C)5→4 | coding (279/591 nt) | uhpA ← | DNA‑binding transcriptional activator UhpA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,850,444 | 0 | C | . | 97.6% | 168.0 / ‑2.4 | 42 | coding (283/591 nt) | uhpA | DNA‑binding transcriptional activator UhpA |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base . (20/21); total (20/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTGCCCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGCCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATGGAGATATCGCAAATA > NC_000913/3850307‑3850574 | tttGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTGccc < 2:569554/140‑1 (MQ=255) ggTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAgcg < 1:26983/140‑1 (MQ=255) acCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAGCGCCTGCt > 2:740747/1‑136 (MQ=255) acCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAGCGCCTGCt < 1:740747/136‑1 (MQ=255) acCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAAc < 2:516421/140‑1 (MQ=255) acCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAACCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAAc < 2:41983/140‑1 (MQ=255) aTGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAgcg < 1:80302/140‑1 (MQ=255) cAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCa > 1:101096/1‑133 (MQ=255) cAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCa < 2:101096/133‑1 (MQ=255) aGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGa < 1:695146/140‑1 (MQ=255) atatCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACg > 1:73609/1‑140 (MQ=255) atatCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACg > 1:768670/1‑140 (MQ=255) atatCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACg > 2:637867/1‑140 (MQ=255) atatCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACg < 1:637867/140‑1 (MQ=255) gCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCa < 2:460752/140‑1 (MQ=255) cGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTG‑CCCCGGGTTAAGCGCCTGGTCAACCAGCGCAAGACTATCGTGAACGGAGAGCAt > 2:217095/1‑140 (MQ=255) aGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAAtc > 2:778652/1‑140 (MQ=255) cccGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGCCATACCt > 1:57613/1‑140 (MQ=255) tGCACCGCAGCAATGCGTTCATGCGAGCTCCAGCGTTTGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGCCATACCTTTCGGCAGCTGGCtt < 1:217095/140‑1 (MQ=255) cGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGCCATACCTTTCGGCAGCTGGCTTagcag > 1:681109/1‑140 (MQ=255) cGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGCCATACCTTTCGGCAGCTGGCTTagcag > 2:731391/1‑140 (MQ=255) cGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGCCATACCTTTCGGCAGCTGGCTTagcag > 2:4395/1‑140 (MQ=255) cGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTAAACGGAGATCATGATCGTCGCCATAGCTTTTGGCAGCTGGCTTagcag > 2:219460/1‑140 (MQ=255) gcagcaATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGCCATACCTTTCGGCAGCTGGCTTagcagc > 2:144680/1‑140 (MQ=255) tGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGCCATACATTTGGGCAGCTGGGTTAGCAGCTCCAGAc > 1:259664/1‑140 (MQ=255) aGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGCCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCg > 2:795987/1‑140 (MQ=255) ccGGGCTACAGCGTTTGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGCCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGatat < 2:73609/140‑1 (MQ=255) ccGGGCTACAGCGTTTGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGCCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGatat < 1:778652/140‑1 (MQ=255) ggCTACAGCGTTTGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGCCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCgg > 1:55336/1‑140 (MQ=255) gCTACAGCGTTTGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGCCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCggg > 1:203378/1‑140 (MQ=255) cTACAGCGTTTGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGCCATACCTTTGGGCAGCTGGCTTAGCAGCTCCCGACCGGAGATATCGGGc > 2:340309/1‑140 (MQ=255) tACAGCGTTTGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGCCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCa < 1:771831/140‑1 (MQ=255) aGCGTTTGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGCCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATgg > 2:184013/1‑140 (MQ=255) cGTTTGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGCCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATGgag < 2:68409/140‑1 (MQ=255) tGGAAAGAAAGCCGCGTTCCCCC‑CGTTAAGCGCCTCCTCTACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGCCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATGGAGatat < 2:259664/140‑1 (MQ=255) tGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGCCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATGGAGatat < 1:731391/140‑1 (MQ=255) tGGAAAGAAAGCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGCCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATGGAGatat < 1:4395/140‑1 (MQ=255) aaagaaagCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGCCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATGGAGat < 2:514612/135‑1 (MQ=255) aaagaaagCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGCCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATGGAGat > 1:514612/1‑135 (MQ=255) aaagaaagCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGCCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATGGAGATATCGc < 2:55336/140‑1 (MQ=255) aagCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGCCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATGGAGATATCGCAAATa < 1:795987/140‑1 (MQ=255) aagCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGCCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATGGAGATATCGCAAATa < 2:57613/140‑1 (MQ=255) aagCCGCGTG‑CCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGCCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATGGAGATATCGCAAATa < 2:203378/140‑1 (MQ=255) | TTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTGCCCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGCCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATGGAGATATCGCAAATA > NC_000913/3850307‑3850574 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |