Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	513,935	(A)7→6	intergenic (+67/‑58)	ybaT → / → cueR	putative transporter YbaT/DNA‑binding transcriptional dual regulator CueR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	513,929	0	A	.	100.0%	283.4 / NA	67	intergenic (+61/‑64)	ybaT/cueR	putative transporter YbaT/DNA‑binding transcriptional dual regulator CueR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (37/30);  total (37/30)

GATATGGATTATTGGCTCACTCTTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGCAAAGCCATTCGCTTCTATGAAGAGAA  >  NC_000913/513796‑514060                                                                                                                                      |                                                                                                                                    gATATGGATTATTGGCTCACTCTTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCaaaaaac                                                                                                                               <  2:196614/140‑2 (MQ=255)     tGGATTATTGGCTCACTCTTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGc                                                                                                                          <  2:713338/140‑1 (MQ=255)            ttGGCTCACTCTTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATAATTGTGGTGGCGTTAGAGATTCGCAATGGC‑TAAAAACGTGCTTGAata                                                                                                                   >  2:392142/1‑140 (MQ=255)             tGGCTCACTCTTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAatat                                                                                                                  <  2:104882/140‑1 (MQ=255)                  cACTCTTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGtt                                                                                                             >  1:565134/1‑140 (MQ=255)                    ctctTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGTTATACGCTCTGGC‑AAAAAACGGGCTTGAATATCTGTTGa                                                                                                           >  2:241623/1‑140 (MQ=255)                    ctctTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGa                                                                                                           <  1:409651/140‑1 (MQ=255)                           tCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCtt                                                                                                    <  1:737619/140‑1 (MQ=255)                              gCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTaa                                                                                                 <  1:97096/140‑1 (MQ=255)                               ccTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAatat                                                                                                                  >  1:183273/1‑122 (MQ=255)                               ccTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAatat                                                                                                                  <  2:183273/122‑1 (MQ=255)                               ccTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAAc                                                                                                >  2:38609/1‑140 (MQ=255)                               ccTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAAc                                                                                                >  1:29481/1‑140 (MQ=255)                                cTTATTGTGGCAATGTTCATGAGGCCCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACa                                                                                               <  1:260272/140‑1 (MQ=255)                                cTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACa                                                                                               <  2:80206/140‑1 (MQ=255)                                cTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACa                                                                                               <  1:38609/140‑1 (MQ=255)                                 ttATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AGAAAACGTGCTTAAAGATCTGTAGAAACCCTTTAACaa                                                                                              >  1:390487/1‑140 (MQ=255)                                 ttATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACaa                                                                                              <  2:597942/140‑1 (MQ=255)                                  tATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTGGCACTGGC‑CAAAAACGCGCTTGAATATCTGATGAAACCCTTTAACaaa                                                                                             >  2:297129/1‑140 (MQ=255)                                   aTTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGaaa                                                                                                         <  2:177047/127‑1 (MQ=255)                                   aTTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGaaa                                                                                                         >  1:177047/1‑127 (MQ=255)                                         gCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGTC‑AAAAAACTTGCATGAATATCTGTTGAAACGCTTTAACAAAGCACagg                                                                                      >  2:260272/1‑140 (MQ=255)                                         gCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACTTGCCTGAATATCTGTTCAAACCCTTTAACAAAGCACagg                                                                                      >  2:295547/1‑140 (MQ=255)                                         gCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACTTGCATGAATATCTGTTCAAACGCTTTAACAAAGCACagg                                                                                      >  2:328472/1‑140 (MQ=255)                                         gCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTaa                                                                                                 <  1:642157/129‑1 (MQ=255)                                         gCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTaa                                                                                                 >  2:642157/1‑129 (MQ=255)                                         gCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACCTGCGTGAATATCTGTTCAAACAATTTAACAAAGCACagg                                                                                      >  2:388030/1‑140 (MQ=255)                                               gTCATGAAGCGCAATAAAACCGTATAACCTCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTAGAAACCATTTAACAAAGCACAGGAGGCGt                                                                                >  1:370320/1‑140 (MQ=255)                                                   tGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTTTTGGAACCCTTTCACAAAGCAAAGGAGGCGTTgcg                                                                            >  2:272723/1‑140 (MQ=255)                                                    gAAGCGCAATAAAACGGTATAATATGTCTTTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTgcgc                                                                           <  1:297129/140‑1 (MQ=255)                                                         gcAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACg                                                                      >  1:4127/1‑140 (MQ=255)                                                                          cATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAg                                                           >  1:817142/1‑134 (MQ=255)                                                                          cATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAg                                                           <  2:817142/134‑1 (MQ=255)                                                                             ctctctGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGc                                                  >  2:144490/1‑140 (MQ=255)                                                                               ctctGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGTCGTGGCGCGAACAATGAGCATCAGCGATGTAGCaa                                                >  2:252287/1‑140 (MQ=255)                                                                                  tgtgCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCaaaaa                                             >  2:677327/1‑140 (MQ=255)                                                                                    tgCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAAtt                                           >  2:491643/1‑140 (MQ=255)                                                                                      cgcAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCAAAGGAGGCGATGCGCGATCGATGAACGTCAGCGATGTAGCAAAAATTAc                                         >  2:273297/1‑140 (MQ=255)                                                                                          gTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATg                                                      <  1:6969/123‑1 (MQ=255)                                                                                          gTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATg                                                      >  2:6969/1‑123 (MQ=255)                                                                                          gTACTTCCTGTATTACTGTGGTCGCCGTCCATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGc                                     <  1:211008/140‑1 (MQ=255)                                                                                             gttCCTGTATTATTGTGGTGGCTGTCGATATTCGCACCGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTg                                  <  1:220818/139‑1 (MQ=255)                                                                                              ttCCTGTATTATTGTAGTGGCGCTCCATAGTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGa                                 <  1:392142/140‑1 (MQ=255)                                                                                                   gTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGACCATCAGCGATGAAGAAAAAATCACCGGCCTGACCAGc                            >  1:246552/1‑140 (MQ=255)                                                                                                   gTATTATTGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGc                            >  1:579643/1‑140 (MQ=255)                                                                                                     attattGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTa                                          <  1:478681/124‑1 (MQ=255)                                                                                                     attattGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTa                                          >  2:478681/1‑124 (MQ=255)                                                                                                     attattGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGCaa                          >  1:14236/1‑140 (MQ=255)                                                                                                     attattGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGCaa                          >  2:678825/1‑140 (MQ=255)                                                                                                     attattGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGCaa                          >  2:618540/1‑140 (MQ=255)                                                                                                       tattGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAGCGATGAACCTCACCGAGGTAGCAAAAATTAGCGGCCTGACCAGCAAAg                        >  2:211193/1‑140 (MQ=255)                                                                                                         ttGTGGTGGCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACTATGAACATGAGCGATGGAGCAAAAAGTACCTGCCTGACCAGCAAAGcc                      >  2:220818/1‑140 (MQ=255)                                                                                                            tggtggCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGCAAAGCCAtt                   <  1:144490/140‑1 (MQ=255)                                                                                                            tggtggCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGACGCGTTGCGCGAACGATGAACATCAGTGATCTAGGAAAAATTACCGGCTTGACCAGCAAAGCCAtt                   >  2:208849/1‑140 (MQ=255)                                                                                                            tggtggCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTG‑AACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGCAAAGCCATTc                  >  2:695839/1‑140 (MQ=255)                                                                                                            tggtggCGGTCGATATTAGCACAGGC‑AAAAAACGTTCTTTAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGCAAAGCCAtt                   <  2:246552/140‑1 (MQ=255)                                                                                                            tggtggCGGTCGATACTCGCACTGGC‑AAATAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGCAAAGCCAtt                   <  1:295547/140‑1 (MQ=255)                                                                                                              gtggCGGTCGATATTCTCACTGTC‑AAAAAACTTTCTTGACTATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGCAAAGCCATTCg                 <  2:370320/140‑1 (MQ=255)                                                                                                              gtggCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGCAAAGCCAt                    <  1:84965/137‑1 (MQ=255)                                                                                                              gtggCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGCAAAGCCAt                    >  2:84965/1‑137 (MQ=255)                                                                                                              gtggCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGCAAAGCCATTCg                 <  2:14236/140‑1 (MQ=255)                                                                                                              gtggCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGCAAAGCCATTCg                 >  2:768306/1‑140 (MQ=255)                                                                                                                ggCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTG‑AACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGCAAAGCCATTCGCtt              <  1:695839/140‑1 (MQ=255)                                                                                                                 gCGGTCGATATTCGCGCTGGC‑ATAAAACGAGCTTGAAAATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGCAAAGCCATTCGCtt              <  1:272723/140‑1 (MQ=255)                                                                                                                 gCGGTCGATATTCGCGCTGGC‑ACAACACGAGCTTGAAAATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGCAAAGCCATTCGCtt              <  1:388030/140‑1 (MQ=255)                                                                                                                 gCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGCAAAg                        >  2:621801/1‑130 (MQ=255)                                                                                                                 gCGGTCGATATTCGCACTGGC‑AAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGCAAAg                        <  1:621801/130‑1 (MQ=255)                                                                                                                       gATATTCGCACTGGC‑GAAAAACTTGTTTGTATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGCAAAGCCATTCGCTTCTATGa        <  1:273297/140‑1 (MQ=255)                                                                                                                             cGCACTGGC‑AAAAAACGTGCTTTAAAATCCGTTGAAAGCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGCAAAGCCATTCGCTTCTATGAAGAGaa  <  1:241623/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                    GATATGGATTATTGGCTCACTCTTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGCAAAGCCATTCGCTTCTATGAAGAGAA  >  NC_000913/513796‑514060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑