Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	715,242	(T)6→5	intergenic (+44/‑170)	pgm → / → ybfP	phosphoglucomutase/lipoprotein YbfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	715,237	0	T	.	100.0%	164.8 / NA	40	intergenic (+39/‑175)	pgm/ybfP	phosphoglucomutase/lipoprotein YbfP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (23/17);  total (23/17)

TCTACTGCGAAAGCTTCCTCGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGATTGTTAGCGAAGTTCTGAAAAACGCGTAAACACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCA  >  NC_000913/715107‑715362                                                                                                                                   |                                                                                                                              tCTACTGCGAAAGCTTCCTCGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGATTGTTAGCGAAGTTCTGAAAAACGCGTAAACACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCC‑TTTTTACGTa                                                                                                                     >  1:595381/1‑140 (MQ=255)   tACTGCGAAAGCTTCCTCGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGATTGTTAGCGAAGTTCTGAAAAACGTGTAAACACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCC‑TTTTTACGTATg                                                                                                                   >  2:418155/1‑140 (MQ=255)    aCTGCGAAAGCTTCCTCGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGATTGTTAGCGAAGTTCTGAAAAACGCGTAAACACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCC‑TTTTTACGTATGa                                                                                                                  >  1:580970/1‑140 (MQ=255)     cagagAAAGCTTCCTCGCTGAATAACATCGCCAGCCGATCGAGAAAGAAGCGGTTGAGACTGTTAGCGAAGTTCTGAAAAACGCGTAAACACGTTTAATAAAAAAAGGGCGGTCGCAAGATCGCCC‑TTTTTACGTATGAc                                                                                                                 <  2:330217/136‑1 (MQ=255)        cGAAAGCTTCCTCGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGATTGTTAGCGAAGTTCTGAAAAACGCGTAAACACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCC‑TTTTTACGTATGACaaa                                                                                                              >  1:461434/1‑140 (MQ=255)         gAAAGCTTCCTCGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGATTGTTAGCGAAGTTCTGAAAAACGCGTAAACACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCC‑TTTTTACGTATGACAAac                                                                                                             >  1:655842/1‑140 (MQ=255)          aaaGCTTCCTCGGTGGAGAACATCGTAAGAAGATTGATAAAGAAGCGGTTGAGATTGTTAGCGAAGTTCTGAAAAACGCGTAAACACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCC‑TTTTTACGTATGACAAaca                                                                                                            <  1:308659/140‑1 (MQ=255)           aaGCTTCCTCGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGATTGTTAGCGAAGTTCTGAAAAACGCGTAAACACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCC‑TTTTTACGTATGACAAacac                                                                                                           >  1:713243/1‑140 (MQ=255)                      gTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGATTGTTAGCGAAGTTCTGAAAAACGCGTAAACACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCC‑TTTTTACGTATGACAAACACAGAATTGCCTg                                                                                                <  1:418155/140‑1 (MQ=255)                                 cGCAAGCAGATTGAGAAAGAAGCGGTTGAGATTGTTAGCGAAGTTCTGAAAAACGCGTAAACACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCC‑TTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGc                                                                                     >  2:761291/1‑140 (MQ=255)                                 cGCAAGCAGATTGAGAAAGAAGCGGTTGAGATTGTTAGCGAAGTTCTGAAAAACGCGTAAACACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCC‑TTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGc                                                                                     >  2:71297/1‑140 (MQ=255)                                 cGCAAGCAGATTGAGAAAGAAGCGGTTGAGATTGTTAGCGAAGTTCTGAAAAACGCGTAAACACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCC‑TTTTTACGTATGACAAACACAGAATTACCTGATGCGCTACGc                                                                                     >  1:342402/1‑140 (MQ=255)                                 cGCAAGCAGATTGAGAAAGAAGCGGTTGAGATTGTTAGCGAAGTTCTGAAAAACGCGTAAACACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCC‑TTGTTATGTATGACAAACATAGAAATGCCTGATGCGCTACGc                                                                                     >  2:264875/1‑140 (MQ=255)                                         gATTGAGAAAGAAGCGGTTGAGATTGTTAGCGAAGTTCTGAAAAACGCGTAAACACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCC‑TTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAgg                                                                             <  2:655842/140‑1 (MQ=255)                                                   gaagCGGTTGAGATTGTTAGCGAAGTTCTGAAAAACGCGTAAACACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCC‑TTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGa                                                                   <  2:713243/140‑1 (MQ=255)                                                   gaagCGGTTGAGATTGTTAGCGAAGTTCTGAAAAACGCGTAAACACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCC‑TTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGa                                                                   <  1:761291/140‑1 (MQ=255)                                                   gaagCGGTTGAGATTGTCAGCGTAGTTCTGAAAAATGCGCAAACACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCC‑TTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGa                                                                   <  2:243123/140‑1 (MQ=38)                                                          ttGAGATTGTTAGCGTAGTTCTAAACAACGCGTAAACCCATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCC‑TTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCa                                                            <  1:260468/140‑1 (MQ=38)                                                              gaTTGTTAGCGAAGTTCTGAAAAACGCGTAAACACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCC‑TTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCa                                                            >  1:66522/1‑136 (MQ=255)                                                              gaTTGTTAGCGAAGTTCTGAAAAACGCGTAAACACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCC‑TTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCa                                                            <  2:66522/136‑1 (MQ=255)                                                                    tAGCGAAGTTCTGAAAAACGCGTAAACACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCC‑TTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGa                                                                   >  1:541557/1‑123 (MQ=255)                                                                    tAGCGAAGTTCTGAAAAACGCGTAAACACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCC‑TTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGa                                                                   <  2:541557/123‑1 (MQ=255)                                                                         aaGTTCTGAAAAACGCGTAAACACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCC‑TTTTTACGTATGACAAACACAGAATTGCCTGATACGCTACGCCTATCAAGCATACGAGGTTGGTTAAATATATTGAATTTaa                                             >  2:391011/1‑140 (MQ=255)                                                                            ttCTGAAAAACGCGTAAACACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCC‑TTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCg                                          <  2:17031/140‑1 (MQ=255)                                                                                 aaaaaCGCGTAAACACATTTAATAAAAAAAGGGCGGCCGCAAGATCGCCC‑TTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGCtttt                                     >  1:18409/1‑140 (MQ=255)                                                                                  aaaaCGCGTAAACACCTTTAATAAAAAAACGGCGGTCTCAAGATCGCCC‑TTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTg                                    <  1:264875/140‑1 (MQ=38)                                                                                   aaaCGCGTAAACACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCC‑TTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGt                                   <  2:580970/140‑1 (MQ=255)                                                                                      cgcgTAAACACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCC‑TTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAgg                                >  1:435338/1‑140 (MQ=255)                                                                                      cgcgTAAACACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCC‑TTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAgg                                >  2:144423/1‑140 (MQ=255)                                                                                       gcgTAAACACATTTAGTAAAAATAGGGCGGTCGCAAGTTCGCCC‑TTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGc                               <  1:391011/140‑1 (MQ=38)                                                                                          tAAACACATTTAATACAAAAAGCGCTGTCTCAAGATCGCCC‑TTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCgg                            <  1:384014/140‑1 (MQ=37)                                                                                                 aTTTAATAAAAAAAGGGCGGTCGCAAGATCGCCC‑TTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATAAATTAAATTTAAGAGATTTTGTAGGCCGGATAAGGc                     >  2:384014/1‑140 (MQ=37)                                                                                                  tttAATAAAAAAAGGGCGGTCGCAAGATCGCCC‑TTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTg                                    >  2:10531/1‑124 (MQ=255)                                                                                                  tttAATAAAAAAAGGGCGGTCGCAAGATCGCCC‑TTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTg                                    <  1:10531/124‑1 (MQ=255)                                                                                                     aataaAAAAAGGGCGGTCGCAAGATCGCCC‑TTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGTCGGATAAGGCGTTc                 >  1:728442/1‑140 (MQ=255)                                                                                                       taaAAAAAGGGCGGTCGCAAGATCGCCC‑TTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCAc               >  1:41154/1‑140 (MQ=255)                                                                                                           aaaaGGGCGGTCGCAAGATCGCCC‑TTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCAcgccg           >  2:145856/1‑140 (MQ=255)                                                                                                                 gCGGTCGCAAGATCGCCC‑TTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCg     >  2:688194/1‑140 (MQ=255)                                                                                                                   ggTCGCAAGATCGCCC‑TTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc   <  1:145856/140‑1 (MQ=255)                                                                                                                    gTCGCAAGATCGCCC‑TTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa  >  1:705506/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                              TCTACTGCGAAAGCTTCCTCGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGATTGTTAGCGAAGTTCTGAAAAACGCGTAAACACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCA  >  NC_000913/715107‑715362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑