Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	723,528	(T)8→7	coding (887/2685 nt)	kdpD ←	sensory histidine kinase KdpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	723,521	0	T	.	100.0%	148.1 / NA	36	coding (894/2685 nt)	kdpD	sensory histidine kinase KdpD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (21/15);  total (21/15)

AATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATG  >  NC_000913/723395‑723647                                                                                                                               |                                                                                                                               aatCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTaa                                                                                                                  <  1:727486/140‑1 (MQ=255)       gCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTg                                                                                                            <  1:296962/140‑1 (MQ=255)             aTTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAg                                                                                                                 >  2:269200/1‑129 (MQ=255)             aTTATGTTCACGGGAATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAg                                                                                                                 <  1:269200/129‑1 (MQ=255)                   ttCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑‑TTTTTTCCGGTAAGCGGTGCAGGGCAGGGGtt                                                                                               <  2:732649/140‑1 (MQ=255)                     cACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGttt                                                                                              <  1:488234/140‑1 (MQ=255)                       cGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCa                                                                                            >  2:778336/1‑140 (MQ=255)                           cATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCATGGCAGGGGTTTCAACAt                                                                                        >  2:286522/1‑140 (MQ=255)                             tAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATac                                                                                      >  1:829640/1‑140 (MQ=255)                                  cACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACcgc                                                                                 <  1:778336/140‑1 (MQ=255)                                   aCTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCAGGTCAGCGGTGCAGGGAAGGGGTTTCAACATACACcgcg                                                                                >  2:320576/1‑140 (MQ=255)                                          cTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTATGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGa                                                                         >  2:606822/1‑140 (MQ=255)                                          cTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAGGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGa                                                                         >  2:820079/1‑140 (MQ=255)                                             tctcTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGc                                                                      >  1:202186/1‑140 (MQ=255)                                                 ttCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTAcc                                                                  >  1:156931/1‑140 (MQ=255)                                                      cTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGcc                                                             >  2:532980/1‑140 (MQ=255)                                                         gATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACg                                                                       <  1:432484/127‑1 (MQ=255)                                                         gATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACg                                                                       >  2:432484/1‑127 (MQ=255)                                                                   tgtTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGACAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAgccg                                                >  2:820975/1‑140 (MQ=255)                                                                    gtTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAgccgc                                               >  1:137949/1‑140 (MQ=255)                                                                    gtTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATAAACCGCGTACCAGACGCAACCCAGCCGTGACGCCAgccgc                                               >  2:288963/1‑140 (MQ=255)                                                                     ttGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAgccgcg                                              >  1:114409/1‑140 (MQ=255)                                                                     ttGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAgccgcg                                              >  2:698743/1‑140 (MQ=255)                                                                         cGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAgccgcgccgc                                          >  2:346286/1‑140 (MQ=255)                                                                           tctcCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGTCAGACGCTACCAAGGCGTGACACCAGCCGCGCCGCTg                                        >  1:389818/1‑140 (MQ=255)                                                                             tcCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTgcg                                      <  2:192560/140‑1 (MQ=255)                                                                                          ccTGCGCCAGACGTATGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGttttt                         <  1:606822/140‑1 (MQ=255)                                                                                             gcgcCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGAc                                 >  1:632034/1‑129 (MQ=255)                                                                                             gcgcCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGAc                                 <  2:632034/129‑1 (MQ=255)                                                                                             gcgcCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGc                      <  2:829640/140‑1 (MQ=255)                                                                                                  aGACGTAAGGCGCTGGGAATTGACCAACG‑TTTTTTTCTGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCg                 <  1:288963/140‑1 (MQ=255)                                                                                                  aGACGTAAGGCGCTGAGAATTGGCCAACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCg                 <  1:320576/140‑1 (MQ=255)                                                                                                  aGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGTTGTGCGGACCAGTTTTTCGCTGCCg                 >  1:208690/1‑140 (MQ=255)                                                                                                            cgcTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGc       <  1:346286/140‑1 (MQ=255)                                                                                                              cTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATggacg     <  1:698743/140‑4 (MQ=255)                                                                                                               tGAGAATTTCCCGACG‑TGTTTTCCCGGTAAGGGTTACAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGa    <  2:375389/140‑1 (MQ=255)                                                                                                               tGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCCGCGCGGAACAGCTTATCGCTGACGGTGTTATGGCCGa    >  1:375389/1‑140 (MQ=255)                                                                                                                 agaATTGCCCGACG‑TTTCTTTCCGGTAAGCTCTACAGGGAAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATg  <  2:389818/140‑1 (MQ=255)                                                                                                                               |                                                                                                                               AATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATG  >  NC_000913/723395‑723647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑