Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,351,196	(G)7→6	intergenic (‑157/+211)	fabI ← / ← ycjD	enoyl‑[acyl‑carrier‑protein] reductase/DUF559 domain‑containing protein YcjD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,351,190	0	G	.	100.0%	108.4 / NA	27	intergenic (‑151/+217)	fabI/ycjD	enoyl‑[acyl‑carrier‑protein] reductase/DUF559 domain‑containing protein YcjD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (13/14);  total (13/14)

TGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGA  >  NC_000913/1351055‑1351319                                                                                                                                        |                                                                                                                                  tgttgATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAggggg                                                                                                                               <  2:189108/140‑1 (MQ=255)      aTGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGACTGCGGCAggggggcgct                                                                                                                          >  1:90557/1‑136 (MQ=255)            gttgtGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTccc                                                                                                                   >  1:739259/1‑140 (MQ=255)              tgtgCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCt                                                                                                                 <  1:518485/140‑1 (MQ=255)                 gCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCAc                                                                                                              >  2:3133/1‑140 (MQ=255)                      aaaaTCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTaa                                                                                                         >  1:467656/1‑140 (MQ=255)                       aaaTCAGGCGATTCGTCGTTTTGGTAAACAGGACGGACAGATAAACGGGTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAAc                                                                                                        <  2:315236/140‑1 (MQ=255)                        aaTCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAAcc                                                                                                       <  1:3133/140‑1 (MQ=255)                         aTCAGGCGATTCGTCGTATTAGCAAACAGTACGAACAGACAAACGGTTCTTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGTGCGCTTTTCCCCTCACCCTAAccc                                                                                                      <  2:231665/140‑1 (MQ=255)                                            tAGTAAACAGTACGAACAGATAAACGGTTATTATACTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAgg                                                                                   <  2:467656/140‑1 (MQ=255)                                                 aaCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGAcc                                                                              <  1:662572/140‑1 (MQ=255)                                                     gTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAt                                                                          <  1:515608/140‑1 (MQ=255)                                                      tACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAtt                                                                         >  1:518072/1‑140 (MQ=255)                                                      tACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAtt                                                                         >  2:470511/1‑140 (MQ=255)                                                        cGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGGATtgt                                                                       >  2:629836/1‑140 (MQ=255)                                                         gAACAGATAAACGGTTATTATAATCATCCTGGCTGTGAGTAGCTATAGTTGCCAGGTCAGACCGGAGCATGCTGCGGCA‑GGGGGGCGCTTTTCCCCCCTCCCTATCCCGCGCCCCAGAGGGGCGAGGGGACCGTATtgtg                                                                      <  2:588032/140‑1 (MQ=255)                                                                tAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCCCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAatat                                                               >  2:564546/1‑140 (MQ=255)                                                                         attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCTTCTCCCCAGAGGGGCGCGGGGACCGAATTGTGCAAATATTGTTAcccc                                                      >  2:317564/1‑140 (MQ=39)                                                                                  aaCCTGGCTGTGAGTGGCTATAGTTACCATGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                             <  1:317564/140‑1 (MQ=38)                                                                                  aaCCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                             <  1:780281/140‑1 (MQ=255)                                                                                        gCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACGGCAATGTACAAATATTATTACCCCAGCAACAAACAGGcc                                       >  2:310269/1‑139 (MQ=38)                                                                                         cTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCCCCCTAACCCCCTCCCCAGAGGGGCGGGGGGACCGTATTGTGCACATAGTTGTACCCCTGCAAAAAACAGGCTc                                      >  1:388160/1‑140 (MQ=255)                                                                                             gAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGACTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATAc                                  <  2:90557/140‑1 (MQ=255)                                                                                              aGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                             >  2:429258/1‑128 (MQ=255)                                                                                              aGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                             <  1:429258/128‑1 (MQ=255)                                                                                              aGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACa                                 <  1:470511/140‑1 (MQ=255)                                                                                                                   cGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCg            >  1:118067/1‑140 (MQ=255)                                                                                                                     aCCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGAt          >  1:93792/1‑140 (MQ=255)                                                                                                                             aGGCTGCGGCA‑GGGGGTAGCTTTTCCCCTCTCCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa  <  2:388160/140‑1 (MQ=38)                                                                                                                             aGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa  <  1:564546/140‑1 (MQ=255)                                                                                                                             aGGCTGCGGCA‑GGGGCGCGCTTTTCGCCTGACCCTAATCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa  <  1:310269/140‑1 (MQ=39)                                                                                                                                        |                                                                                                                                  TGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGA  >  NC_000913/1351055‑1351319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑