Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,949,993	(T)5→6	coding (752/1332 nt)	argA →	N‑acetylglutamate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,949,988	1	.	T	94.2%	167.9 / 2.1	52	coding (747/1332 nt)	argA	N‑acetylglutamate synthase
Reads supporting (aligned to +/- strand):  ref base . (0/3);  new base T (26/23);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 2.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGGTTTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑C‑TT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAG  >  NC_000913/2949858‑2950122                                                                                                                                     |                                                                                                                                         ttGGTTTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑C‑tt‑tt‑ttgcg                                                                                                                               <  1:727847/140‑5 (MQ=255)      ttttGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGgcg                                                                                                                           <  1:181592/140‑1 (MQ=255)      ttttGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACGCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGgcg                                                                                                                           <  1:766520/140‑1 (MQ=255)                          cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCa                                                                                                         >  1:578007/1‑138 (MQ=255)                          cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCa                                                                                                         <  2:578007/138‑1 (MQ=255)                          cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAgc                                                                                                       >  1:765266/1‑140 (MQ=255)                          cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAgc                                                                                                       >  2:814426/1‑140 (MQ=255)                          cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAgc                                                                                                       >  1:87695/1‑140 (MQ=255)                          cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAgc                                                                                                       >  2:531204/1‑140 (MQ=255)                          cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAgc                                                                                                       >  2:743292/1‑140 (MQ=255)                          cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAgc                                                                                                       >  2:615082/1‑140 (MQ=255)                          cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAgc                                                                                                       >  2:630836/1‑140 (MQ=255)                             tAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAgcggc                                                                                                    <  2:148295/140‑1 (MQ=255)                              aaTGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAgcggcg                                                                                                   <  2:15111/140‑1 (MQ=255)                                taatgacgGTGATATTGCCTCCAAACTTTTCTCTACCGACGCGCAAGCTCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGgcgtg                                                                                                 <  2:219587/136‑1 (MQ=255)                                tGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGgcgtg                                                                                                 <  1:630836/140‑1 (MQ=255)                                     acgGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCg                                                                                            <  1:108292/140‑1 (MQ=255)                                       ggTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCt                                                                                          <  1:661665/140‑1 (MQ=255)                                         tGATATTGTCTCCGAACTTTTCCGTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGt                                                                                        >  1:577097/1‑140 (MQ=255)                                         tGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGGAAGGCTCCCGCAGCGGCGTGCGTCGCTGt                                                                                        >  1:315677/1‑140 (MQ=255)                                                  ctcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATc                                                                               >  1:828091/1‑140 (MQ=255)                                                   tcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAAt                                                                                <  2:798942/138‑1 (MQ=255)                                                   tcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAAt                                                                                >  1:798942/1‑138 (MQ=255)                                                    ccGAACTTTTCCCTATCGAAGCCCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAg                                                                             <  1:394678/140‑1 (MQ=255)                                                      gAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGtt                                                                           >  1:115949/1‑140 (MQ=255)                                                           tttCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCAt                                                                                     <  1:803737/125‑1 (MQ=255)                                                           tttCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCAt                                                                                     >  2:803737/1‑125 (MQ=255)                                                                 tAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGAt                                                                >  1:514736/1‑140 (MQ=255)                                                                  aaCGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATg                                                               <  2:519604/140‑1 (MQ=255)                                                                   aCGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATgg                                                              >  2:5102/1‑140 (MQ=255)                                                                        gcgcaagcgcGGGTAGATGCCCAGGAAGAGACAGGCGATTACACGTCCTGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcgcgc                                                         <  2:346602/140‑1 (MQ=255)                                                                        gcgcaagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGGCGCTGTCATTTAATTAGTTATCAGGAAGATGgcgcgc                                                         >  1:331276/1‑140 (MQ=255)                                                                        gcgcaagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGCGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcgcgc                                                         <  2:828091/140‑1 (MQ=255)                                                                         cgcaagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCt                                                        <  2:657913/140‑1 (MQ=255)                                                                             agcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcg                                                            >  1:176934/1‑132 (MQ=255)                                                                             agcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcg                                                            <  2:176934/132‑1 (MQ=255)                                                                             agcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTCTCATTTATTCAGTTATCAGGACGATGGCGCGCTGTTg                                                    >  1:346602/1‑140 (MQ=255)                                                                                          gCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTtct                                       <  1:531204/140‑1 (MQ=255)                                                                                            ccAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCa                                     <  1:814426/140‑1 (MQ=255)                                                                                               ggAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCAcgc                                  >  2:703911/1‑140 (MQ=255)                                                                                               ggAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCACTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCAcgc                                  >  2:192813/1‑140 (MQ=255)                                                                                                 aaGAGAAAGGCGATTGCAACTCCGGAACGCTGCG‑C‑TTCTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGa                                <  1:245139/140‑1 (MQ=255)                                                                                                 aaGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGa                                <  1:675885/140‑1 (MQ=255)                                                                                                        aGGCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATc                         >  1:667234/1‑140 (MQ=255)                                                                                                          gCGATTACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCgg                       <  1:5102/140‑1 (MQ=255)                                                                                                              ttACAACTCCGGTACGGTGCGCC‑TT‑TT‑TGCGGGGCACAGCGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACg                   <  2:315677/140‑1 (MQ=255)                                                                                                              ttACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACg                   <  2:87695/140‑1 (MQ=255)                                                                                                              ttACAACTCCGGTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACg                   <  2:577097/140‑1 (MQ=255)                                                                                                                  aaCTCCGGTACGGTGCG‑A‑TT‑TTATGCGTGGCTCAGAGAATGCCTGCCGAAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGa               <  2:331276/140‑1 (MQ=255)                                                                                                                         gTACGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGAt        >  1:825415/1‑140 (MQ=255)                                                                                                                            cGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGAtt             >  2:56763/1‑132 (MQ=255)                                                                                                                            cGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGAtt             <  1:56763/132‑1 (MQ=255)                                                                                                                            cGGTGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGa     >  1:546317/1‑140 (MQ=255)                                                                                                                               tGCG‑CTTT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAg  >  1:601685/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                         TTGGTTTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑C‑TT‑TT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAG  >  NC_000913/2949858‑2950122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑