Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,362,973	(T)6→7	coding (167/2382 nt)	yhcD →	putative fimbrial usher protein YhcD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,362,967	1	.	T	100.0%	197.6 / NA	57	R54L (CGT→CTT)	yhcD	putative fimbrial usher protein YhcD
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (40/17);  total (40/17)

CTACACCATTGGTTTTGCCTTTTCTCCCCCAGCCAATGCAGATGGTATAGAGATTGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑G‑TTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTA  >  NC_000913/3362830‑3363094                                                                                                                                            |                                                                                                                                cTACACCATTGGTTTTGCCTTTTCTCCCCCAGCCAATGCAGATGGTATAGAGATTGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑G‑tt                                                                                                                               <  1:722217/140‑1 (MQ=255)  tACACCATTGGTTTTGCCTTTTCTCCCCCAGCCAATGCAGATGGTATAGAGATTGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑G‑ttt                                                                                                                              >  2:591815/1‑140 (MQ=255)    cacCATTGGTTTTGCCTTTTCTCCCCCAGCCAATGCAGATGGTATAGAGATTGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGCTCAGCCC‑G‑ttttt                                                                                                                            >  2:5629/1‑140 (MQ=255)       cATTGGTTTTGCCTTTTCTCCCCCAGCCAATGCAGATGGTATAGAGATTGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑G‑tttttttg                                                                                                                         <  1:729763/140‑3 (MQ=255)        aTTGGTTTTGCCTTTTCTCCCCCAGCCAATGCAGATGGTATAGAGATTGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑G‑tttttttgc                                                                                                                        <  2:547677/140‑4 (MQ=255)         ttGGTTTTGCCTTTTCTCCCCCAGCCAATGCAGATGGTATAGAGATTGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑G‑tttttttgcc                                                                                                                       >  2:105614/1‑136 (MQ=255)         ttGGTTTTGCCTTTTCTCCCCCAGCCAATGCAGATGGTATAGAGATTGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑G‑tttttttgcc                                                                                                                       >  2:704033/1‑136 (MQ=255)         ttGGTTTTGCCTTTTCTCCCCCAGCCAATGCAGATGGTATAGAGATTGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑G‑tttttttgcc                                                                                                                       >  2:94105/1‑136 (MQ=255)         ttGGTTTTGCCTTTTCTCCCCCAGCCAATGCAGATGGTATAGAGATTGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑G‑tttttttgcc                                                                                                                       >  2:166814/1‑136 (MQ=255)         ttGGTTTTGCCTTTTCTCCCCCAGCCAATGCAGATGGTATAGAGATTGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑G‑tttttttgcc                                                                                                                       >  2:558093/1‑136 (MQ=255)         ttGGTTTTGCCTTTTCTCCCCCAGCCAATGCAGATGGTATAGAGATTGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑G‑tttttttgcc                                                                                                                       >  1:769601/1‑136 (MQ=255)         ttGGTTTTGCCTTTTCTCCCCCAGCCAATGCAGATGGTATAGAGATTGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATAGATCGCATCATTTTTGCCGTTCAGCCC‑G‑tttttttgcc                                                                                                                       >  1:399976/1‑136 (MQ=255)         ttGGTTTTGCCTTTTCTCCCCCAGCCAATGCAGATGGTATAGAGATTGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATAAATCGCATGATTGTTTCCGTTCGGCCC‑G‑tttttttgcc                                                                                                                       >  1:210697/1‑136 (MQ=255)                 gCCTTTTCTCCCCCAGCCAATGCAGATGGTATAGAGATTGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAat                                                                                                                >  1:35777/1‑140 (MQ=255)                  ccTTTTCTCCCCCAGCCAATGCAGATGGTATAGAGATTGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAata                                                                                                               <  1:591815/140‑1 (MQ=255)                   cTTTTCTCGCCCAGCCAATGCAGATGGTATAGAGATTGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAatat                                                                                                              <  1:94105/140‑1 (MQ=255)                    ttttCTCCCCCAGCCAATGCAGATGGTATAGAGATTGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATAtt                                                                                                             <  1:464227/140‑1 (MQ=255)                    ttttCTCCCCCAGCCAATACAGATGGTATAGAGATTGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATAtt                                                                                                             <  1:64819/140‑1 (MQ=255)                      ttCTCCCCCAGCCAATTAAGATAGTATAGAGGTTGTCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCa                                                                                                           <  2:372001/140‑1 (MQ=255)                      ttCTCCCCCAGCCAATGCAGATGGTATAGAGATTGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑G‑tttttttgc                                                                                                                        <  2:679964/126‑4 (MQ=255)                      ttCTCCCCCAGCCAATGCAGATGGTATAGAGATTGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑G‑tttttttgc                                                                                                                        >  1:679964/1‑123 (MQ=255)                        ctcCCCCAGCCAATGCAGATGGTATAGAGATTGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACt                                                                                                         <  1:558093/140‑1 (MQ=255)                          cccccAGCCAATGCAGATGGTATAGAGATTGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGa                                                                                                       <  2:752651/140‑1 (MQ=255)                               aGCCAATGCAGATGGTATAGAGATTGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTa                                                                                                  <  1:203998/140‑1 (MQ=255)                               aGCCAATGCAGATGGTATAGAGATTGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTa                                                                                                  <  1:166814/140‑1 (MQ=255)                                 ccAATGCAGATGGTATAGAGATTGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATAGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATc                                                                                                >  2:80737/1‑140 (MQ=255)                                           tGGTATAGAGATTGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGc                                                                                      >  1:483960/1‑140 (MQ=255)                                                taGAGATTGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCCGG‑TTTTTTGCCAGGGGAAGATTCACTGATAGTATCAGTAAATGGCGaaaa                                                                                 >  2:210851/1‑140 (MQ=255)                                                taGAGATTGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGAGAAAATTCACTGTTAGTATCAGTAAATGGCGaaaa                                                                                 >  2:373690/1‑140 (MQ=255)                                                  gagaTTGCCGCTGTTGGATTTGGTCGGGAAATAGTTAAAACACTAGGTTTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGaaaaaa                                                                               <  2:399976/140‑1 (MQ=255)                                                    gaTTGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGCTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGaaaaaaaa                                                                             <  1:5629/140‑1 (MQ=255)                                                       tGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGTTAGGATAAGTAAATGGCGAAAAAAAAGGc                                                                          >  1:231932/1‑140 (MQ=255)                                                       tGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGc                                                                          >  1:198977/1‑140 (MQ=255)                                                        gCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGGCAGGTGAATATTCACTTATAGTATCGGTAAATGGCGAAAAAAAAGGCa                                                                         >  2:277570/1‑140 (MQ=255)                                                        gCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCa                                                                         >  1:156785/1‑140 (MQ=255)                                                        gCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCa                                                                         >  2:718851/1‑140 (MQ=255)                                                        gCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCa                                                                         >  2:758376/1‑140 (MQ=255)                                                                             gAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTtgatg                                                    >  2:553083/1‑140 (MQ=255)                                                                              aaaCATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTtgatga                                                   >  2:489863/1‑140 (MQ=255)                                                                                     aaaaTCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGc                                            <  2:156785/140‑1 (MQ=255)                                                                                       aaTCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGa                                          >  1:656301/1‑140 (MQ=255)                                                                                           aCTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACAtt                                      >  2:715070/1‑140 (MQ=255)                                                                                            cTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGGAACATTGCTACGCGCTTTGATGAAAATGGCGACAttt                                     >  1:315498/1‑140 (MQ=255)                                                                                                        ccTAATATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTAGATGAAAAGGGCGGCATTTGTCTTGATCAgg                         >  2:311811/1‑140 (MQ=255)                                                                                                        ccTAATATATAGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAgg                         <  1:80737/140‑1 (MQ=255)                                                                                                         cTAATATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGc                        >  1:715296/1‑140 (MQ=255)                                                                                                          tAATATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCa                       >  1:548952/1‑140 (MQ=255)                                                                                                           aaTATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACAt                                       >  1:791809/1‑123 (MQ=255)                                                                                                           aaTATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACAt                                       <  2:791809/123‑1 (MQ=255)                                                                                                           aaTATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCAt                      >  2:158193/1‑140 (MQ=255)                                                                                                           aaTATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCAt                      >  2:60932/1‑140 (MQ=255)                                                                                                           aaTATATCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCAt                      >  1:94272/1‑140 (MQ=255)                                                                                                                atCGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGGTGAAAATGGCGACATTTGTCTGGATCAGGCATTTCTg                 >  1:257835/1‑140 (MQ=255)                                                                                                                  cGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGTCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGca               >  1:11709/1‑140 (MQ=255)                                                                                                                  cGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGa                              <  2:480728/125‑1 (MQ=255)                                                                                                                  cGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGa                              >  1:480728/1‑125 (MQ=255)                                                                                                                  cGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGca               >  2:800714/1‑140 (MQ=255)                                                                                                                  cGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGca               >  2:105443/1‑140 (MQ=255)                                                                                                                  cGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGca               >  2:34622/1‑140 (MQ=255)                                                                                                                  cGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGca               >  2:154375/1‑140 (MQ=255)                                                                                                                  cGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGca               >  2:105264/1‑140 (MQ=255)                                                                                                                  cGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGca               >  1:767444/1‑140 (MQ=255)                                                                                                                  cGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGca               >  1:673360/1‑140 (MQ=255)                                                                                                                  cGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTAAGCGCTTTGATGACAATAACGACATTTGTCTGTATCAGGCATTTCTGca               >  2:393233/1‑140 (MQ=255)                                                                                                                  cGCATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTAAGCGCTTTGATGAAAATGGCGACATTGGTCTGGATCAGGCATTTCTGca               >  2:334256/1‑140 (MQ=255)                                                                                                                    cATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGcaac             >  2:162844/1‑140 (MQ=255)                                                                                                                    cATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGcaac             >  1:81906/1‑140 (MQ=255)                                                                                                                    cATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGcaac             >  2:762759/1‑140 (MQ=255)                                                                                                                    cATTATTTTTCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGcaac             >  1:570702/1‑140 (MQ=255)                                                                                                                           tttCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCgg      <  2:102558/140‑1 (MQ=255)                                                                                                                           tttCCCGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCgg      <  2:94272/140‑1 (MQ=255)                                                                                                                              cccGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACGTTTGTCTAGATGAGGCATTTCGGCAACAAGCCGGttt   >  1:223683/1‑140 (MQ=255)                                                                                                                               ccGTTCAGCCC‑GTTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTa  >  2:681940/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                CTACACCATTGGTTTTGCCTTTTCTCCCCCAGCCAATGCAGATGGTATAGAGATTGCCGCTGTTGATTTTGATCGGGAAACATTAAAATCACTAGGTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCC‑G‑TTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTA  >  NC_000913/3362830‑3363094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑