| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,462,844 | (G)6→7 | coding (501/588 nt) | gspJ → | Type II secretion system protein GspJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,462,838 | 1 | . | G | 90.6% | 169.0 / 10.0 | 53 | coding (495/588 nt) | gspJ | Type II secretion system protein GspJ |
| Reads supporting (aligned to +/- strand): ref base . (1/4); new base G (22/26); total (23/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTACTTCTGTGGATGGCAGGAAGGATCAACCCGCCCAGGCGATGCTGGAACATGTCGAGAGCTTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATAT‑GG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCAT > NC_000913/3462699‑3462967 | cagcTTCTGTGGATGGCAGGAACGATCAACCTGCCTAGGTGATGCTGGTACATGTCGAGAGCTTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAatat < 2:231359/137‑1 (MQ=255) gCAGGAAGGATCAACCCGCCCAGGCGATGCTGGAACATGTCGAGAGCTTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAgcg < 1:20408/140‑1 (MQ=255) gCAGGAAGGATCAACCCGCCCAGGCGATGCTGGAACATGTCGAGAGCTTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAgcg < 1:561680/140‑1 (MQ=255) ggaaggaTCAACCCGCCCAGGCGATGCTGGAACATGTCGAGAGCTTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATAT‑GG‑GG‑GG‑TTACAGCGCCGt < 2:433290/140‑1 (MQ=255) aaggaTCAACCCGCCCAGGCGACGCAGGATCATGTCGCGAGCTTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGtt < 1:245045/140‑1 (MQ=255) tCAACCCGCCCAGGCGATGCTGGAACATGTCGAGAGCTTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCa > 1:110679/1‑140 (MQ=255) cAACCCGCCCAGGCGATGCTGGAACATGTCGAGAGCTTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCAt < 1:149541/140‑1 (MQ=255) cAACCCGACCAGGCGATGCTGGAACATGTCGAGAGCGTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCAt < 2:503494/140‑1 (MQ=255) aCCCGCCCAGGCGATGCTGGAACATGTCGAGAGCTTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTa > 1:16098/1‑140 (MQ=255) cccGCCCAGGCGATGCTGGAACATGTCGAGAGCTTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACCTCTGCAGACTCATCAAGAT‑GG‑GG‑GGCTTACCGCGCCGTTTTGCATTAc > 2:290668/1‑140 (MQ=255) cccAGGCGATGCTGGAACATGTCGAGAGCTTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGa > 1:136971/1‑140 (MQ=255) gCGATGCTGGAACATGTCGAGAGCTTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGt > 1:760629/1‑140 (MQ=255) aTGCTGGAACATGTCGAGAGCTCTCTTCTGGAAAGTAACCGCGGGGAATGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAg < 2:336865/140‑1 (MQ=255) gCTGGAACATGTCGAGAGCTTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCg > 1:650936/1‑140 (MQ=255) tGTCGAGAGCTTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCGTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAagag > 2:180136/1‑140 (MQ=255) tGTCGAGAGCTTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCAGTTGTGCATTACATGAGGAGTTAGCGAGAGAagag > 2:227508/1‑140 (MQ=255) gagaGCTTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGc < 2:627214/140‑1 (MQ=255) gagaGCTTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGc < 1:744888/140‑1 (MQ=255) agagCTTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGcc > 1:653597/1‑140 (MQ=255) tcttctGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAg > 1:555121/1‑140 (MQ=255) tcttctGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAg > 1:580293/1‑140 (MQ=255) tctGCAAAGTAACAGCGGCGAAAGCCAGGACCTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACg < 2:760629/140‑1 (MQ=255) aaaGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGc < 2:110679/140‑1 (MQ=255) aGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCg < 1:623225/140‑1 (MQ=255) aGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCg < 2:662328/140‑1 (MQ=255) aCAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGaa > 1:416770/1‑140 (MQ=255) gCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGaataa < 1:474998/140‑1 (MQ=255) gCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGaataa < 2:555121/140‑1 (MQ=255) ggggAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAataata < 2:653597/140‑1 (MQ=255) ggggAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAataata < 2:428187/140‑1 (MQ=255) gggAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAAtaataa > 1:483659/1‑140 (MQ=255) gAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAAcc < 1:778988/140‑1 (MQ=255) cAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑GTACAGCGCCGTTTTTCCTTACCCGAGCAGGTAACGGGAGAAGAGTCGCCAGCTCCGACGCCGGCCGGGAATAATAACCATGaaa > 2:778988/1‑139 (MQ=255) ccTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGaacaa > 2:581914/1‑140 (MQ=255) tcAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACgcggc > 1:703310/1‑140 (MQ=255) cAGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACgcggcg < 2:650936/140‑1 (MQ=255) aGTCACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATTATAACCATGAATAATGAACAACGCGGCGt > 1:282357/1‑140 (MQ=255) cACGTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTcgc < 2:34201/140‑1 (MQ=255) gcGTTACATCTGCAGACCCAGCCATAT‑GG‑GGTGG‑TCACACCGCCGTTTCGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTcgcg < 2:216928/139‑1 (MQ=255) gTTACATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCgct < 2:740478/140‑1 (MQ=255) acagcATCTGCAGACTCCGCAATAT‑GG‑GG‑GGCTTACAGCGCCGTTTTGAATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCgct < 2:310118/136‑1 (MQ=255) aCATCTGCAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCgctgct < 1:581914/140‑1 (MQ=255) aTCTGCAGACTCAGCAATAT‑GGTGG‑GG‑TTACCGCGACGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCa < 2:282357/140‑1 (MQ=255) cAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTcgcg < 2:124139/128‑1 (MQ=255) cAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTcgcg > 1:124139/1‑128 (MQ=255) cAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCgct > 2:81478/1‑130 (MQ=255) cAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCgct < 1:81478/130‑1 (MQ=255) cAGACTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCAGGAATAATGAACTACTCGGCGTGGCGCTGCTCATTGtc > 1:216928/1‑139 (MQ=255) cTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTcgcg > 1:80915/1‑124 (MQ=255) cTCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTcgcg < 2:80915/124‑1 (MQ=255) tCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATCATGAACAACGCAGCATCGCGCAGCTCATTGTAcctgt > 1:310118/1‑136 (MQ=255) tCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCgct < 2:15676/125‑1 (MQ=255) tCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCgct > 1:15676/1‑125 (MQ=255) tCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTg > 2:659465/1‑133 (MQ=255) tCAGCAATATGGG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTg < 1:659465/133‑1 (MQ=255) | GTACTTCTGTGGATGGCAGGAAGGATCAACCCGCCCAGGCGATGCTGGAACATGTCGAGAGCTTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATAT‑GG‑GG‑GG‑TTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCAT > NC_000913/3462699‑3462967 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |