| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,834,428 | (G)6→7 | coding (110/2319 nt) | yicI ← | alpha‑D‑xyloside xylohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,834,422 | 1 | . | G | 95.0% | 131.0 / ‑0.3 | 40 | coding (116/2319 nt) | yicI | alpha‑D‑xyloside xylohydrolase |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base G (23/15); total (25/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
AGCGGATAATGAGGACCGTTATTCAGCGCCCCCTGAAAATGCTCAATCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGG‑GGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAGTTTCCATCGCTAATTTTCATTGCCGTTCCTTAGTT > NC_000913/3834287‑3834552 | aGCGGATAATGAGGACCGTTATTCAGCGCCCCCTGAAAATGCTCAATCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑gggg > 2:428895/1‑140 (MQ=255) ggATAATGAGGACCGTTATTCAGCGCCCCCTGAAAATGCTCAATCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑gggg‑ggg < 1:480526/140‑2 (MQ=255) ggATAATGAGGACCGTTATTCAGCGCCCCCTGAAAATGCTCAATCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑gggg‑ggg < 1:465846/140‑2 (MQ=255) aTAATGAGGACCGTTATTCAGCGCCCCCTGAAAATGCTCAATCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑gggg‑gggca > 1:578628/1‑137 (MQ=255) aTGAGGACCGTTATTCAGCGCCCCCTGAAAATGCTCAATCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGG‑Ggcagca < 2:615427/140‑1 (MQ=255) ccGTTATTCAGCGCCCCCTGAAAATGCTCAATCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGTTACGTTCCCGCTCATCACGGGGG‑GGCAGCATAGacca > 1:265999/1‑140 (MQ=255) ccGTTATTCAGCGCCCCCTGAAAATGCTCAATCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGG‑GGCAGCATAGacca > 1:649531/1‑140 (MQ=255) cGTTATTCAGCGCCCCCTGAAAATGCTCAATCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGG‑GGCAGCATAGaccac > 2:779543/1‑140 (MQ=255) ttATTCAGCGCCCCCTGCAAATCCTCAATCCCCACCCCGCCAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGG‑GGCAGCATAGAccacca < 2:238065/140‑1 (MQ=255) ttATTCAGCGCCCCCTGAAAATGCTCAATCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGG‑GGCAGCATAGAccacca > 1:540737/1‑140 (MQ=255) ttATTCAGCGCCCCCTGAAAATGCTCAATCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑gggg < 2:624318/122‑1 (MQ=255) ttATTCAGCGCCCCCTGAAAATGCTCAATCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑gggg > 1:624318/1‑122 (MQ=255) cgcCCCCTGAAAATGCTCAATCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGGACATCACGGGGG‑GGAAGCATAGACCACCATTTCATTa > 1:238065/1‑140 (MQ=255) cgcCCCCTGAAAATGCTCAATCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGTGGTAGCATAGACCACCATTTCATTa > 1:231176/1‑140 (MQ=255) cgcCCCCTGAAAATGCTCAATCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGGCGGCAGCATAGACCACCATTTCATTa > 2:423061/1‑140 (MQ=255) cccccTGAAAATGCTCAATCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGG‑GGCAGCATAGACCACCATTTCATTATc > 2:715676/1‑140 (MQ=255) cccTGAAAATGCTCAATCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGG‑GGCAGCATAGACCACCATTTCATTATCct < 2:776511/140‑1 (MQ=255) tGCTCAATCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGG‑GGCAGCATCGACAACGATTTCAGTATCCTGCTGTTCaa > 1:268818/1‑140 (MQ=255) tGCTCAATCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGG‑GGCAGCATAGACCACCATTTCATTATCCTGCTGTTCaa > 1:531267/1‑140 (MQ=255) aaTCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGG‑GGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCg < 1:428895/140‑1 (MQ=255) aaTCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGG‑GGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCg < 2:649531/140‑1 (MQ=255) aaTCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGG‑GGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCg < 1:779543/140‑1 (MQ=255) tCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGG‑GGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGaa > 1:822238/1‑140 (MQ=255) aTACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGG‑GGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGt < 1:715676/140‑1 (MQ=255) cTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGG‑GGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAAt > 1:534551/1‑140 (MQ=255) ttCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGG‑GGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATc > 2:688208/1‑140 (MQ=255) cTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGG‑GGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCaaa > 1:107164/1‑140 (MQ=255) gagaAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGG‑GGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGc > 1:38490/1‑140 (MQ=255) agaAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGG‑GGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGcc > 2:65526/1‑140 (MQ=255) agaAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGG‑GGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGcc > 1:42578/1‑140 (MQ=255) cAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGG‑GGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATc < 2:531267/140‑1 (MQ=255) aCGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACACACATCACGGGGG‑GGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCaa > 2:128350/1‑140 (MQ=255) ggCGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGG‑GGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCGGGTTGAATCAACCAGTTTCCAt > 2:271722/1‑140 (MQ=255) ggCGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGG‑GGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAGTTTCCAt < 1:65526/140‑1 (MQ=255) ggCGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGG‑GGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGGGAAGCGACCTGAGGCCGAGTTAAATCAACCAGTTTCCAt > 2:404518/1‑140 (MQ=255) cGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGG‑GGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAAGCAAATTGAGGCAAGGTTGAATAAACCAGTTTCCATCg > 1:323951/1‑140 (MQ=255) aTCAAGCTGCCAGGTACGTTCACGCACATCACGGGGG‑GGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAGTTTCCATCGCTa < 2:540737/140‑1 (MQ=255) cTGCCAGGTACGTTCACGCACATCACGGGGG‑GGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAGTTTCCATCGCTAATTTTc > 2:143150/1‑140 (MQ=255) tGCCAGGTACGTTCACGCACATCACGGGGG‑GGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGTGGCGAGGGTGAATCAAGCAGTATCCATCGCTAATTTTCa > 2:233264/1‑140 (MQ=255) ccAGGTACGTTCACGCACATCACGGGGG‑GGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAGTTTCCATCGCTAATTTTCAtt > 1:553792/1‑140 (MQ=255) gTACGTTCACGCACATCTCGGGGG‑GGCAGCAAAGACCACCATTTCATTGTCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAGTTTCCATCGCTAATTTTCATTGCCg < 2:268818/140‑1 (MQ=255) gTACGTTCACGCACATCACGGGGG‑GGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAGTTTCCATCGCTAATTTTCATTGCCg < 1:423061/140‑1 (MQ=255) gTACGTTCACGCACATCACGGGGG‑GGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAGTTTCCATCGCTAATTTTCATTGCCg < 2:265999/140‑1 (MQ=255) gTACGTTCACGCACATCACGGGGG‑GGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAGTTTCCATCGCTAATTTTCATTGCCg < 1:688208/140‑1 (MQ=255) gTACGTTCACACACATCACGGGGG‑GGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAGTTTCCATCGCTAATTTTCATTGCCg < 1:128350/140‑1 (MQ=255) ttCACGCACATCACGGGGG‑GGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAGTTTCCATCGCTAATTTTCATTGCCGTTCCt < 2:107164/140‑1 (MQ=255) gCACATCACGGGGG‑GGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAGTTTCCATCGCTAATTTTCATTGCCGTTCCTTAGtt > 2:827608/1‑140 (MQ=255) | AGCGGATAATGAGGACCGTTATTCAGCGCCCCCTGAAAATGCTCAATCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCAC‑GGGG‑GGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAGTTTCCATCGCTAATTTTCATTGCCGTTCCTTAGTT > NC_000913/3834287‑3834552 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |