Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,836,534	(C)6→7	intergenic (+218/‑419)	selC → / → setC	tRNA‑Sec/putative arabinose exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,836,528	1	.	C	100.0%	209.2 / NA	59	intergenic (+212/‑425)	selC/setC	tRNA‑Sec/putative arabinose exporter
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (22/37);  total (22/37)

AATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTG‑‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACTCGCCCCAGAACACCCTTTTTTATACGACTATTCTATTTCTTTAACCGCTGGCGAAAAAAACTGACTATAAACTTCA  >  NC_000913/3836389‑3836662                                                                                                                                             |                                                                                                                                        aaTCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTg                                                                                                                                          >  1:44754/1‑140 (MQ=255) aaTCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTg                                                                                                                                          >  2:685480/1‑140 (MQ=255) aaTCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTg                                                                                                                                          >  2:158303/1‑140 (MQ=255)  aTCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGCATGCGCCGTGTTTATCTGCAGGTGATTTG‑‑c                                                                                                                                       <  2:502028/140‑1 (MQ=255)    cGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTG‑‑ccc                                                                                                                                     <  2:495491/140‑1 (MQ=255)    cGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTG‑‑ccc                                                                                                                                     <  2:767793/140‑1 (MQ=255)      gCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTG‑‑ccccc                                                                                                                                   >  1:113042/1‑140 (MQ=255)      gCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTG‑‑ccccc                                                                                                                                   >  1:567180/1‑140 (MQ=255)      gCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTG‑‑ccccc                                                                                                                                   <  1:478252/140‑1 (MQ=255)      gCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTG‑‑ccccc                                                                                                                                   >  2:86724/1‑140 (MQ=255)       cTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTG‑‑cccccc                                                                                                                                  <  1:426367/140‑1 (MQ=255)                 aCCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGActc                                                                                                                         <  1:556730/140‑1 (MQ=255)                 aCCTGGTCTAAGGCTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGGCGTTTTTGTTTGCAGGGGATTTGC‑CCCCCCTGCTGActc                                                                                                                         >  2:214599/1‑140 (MQ=255)                     ggTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCa                                                                                                                      >  1:461504/1‑139 (MQ=255)                     ggTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCa                                                                                                                      <  2:461504/139‑1 (MQ=255)                        cTAAGGTTTTGTGATGGCCGATGAGCGGCTGAATACTCCTCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCa                                                                                                                  <  2:364431/140‑1 (MQ=255)                           aGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACt                                                                                                               >  1:606485/1‑140 (MQ=255)                               tttGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTAt                                                                                                           <  2:645895/140‑1 (MQ=255)                                ttGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATc                                                                                                          >  1:590647/1‑140 (MQ=255)                                 tgtgATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGcc                                                                                                                   <  1:828324/130‑1 (MQ=255)                                 tgtgATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGcc                                                                                                                   >  2:828324/1‑130 (MQ=255)                                 tgtgATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTAt                                                                                                           <  2:615862/138‑1 (MQ=255)                                 tgtgATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTAt                                                                                                           >  1:615862/1‑138 (MQ=255)                                 tgtgATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCg                                                                                                         >  2:456605/1‑140 (MQ=255)                                    gATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCa                                                                                                      <  1:620351/140‑1 (MQ=255)                                      tGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCAtt                                                                                                    <  2:37349/140‑1 (MQ=255)                                      tGGACGATGACCGGCTGCAGAAGCCGCCGGCGGTTTCTTTTGTCCTCGGACGGATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGAAGGTGACTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCAtt                                                                                                    <  2:384160/140‑1 (MQ=255)                                       ggACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTa                                                                                                   >  1:133809/1‑140 (MQ=255)                                          cGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAgg                                                                                                >  1:638115/1‑140 (MQ=255)                                          cGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAgg                                                                                                >  2:785958/1‑140 (MQ=255)                                           gATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGt                                                                                               >  1:440044/1‑140 (MQ=255)                                            aTGAGCGGCTGAAGAATCCGCCCGTCGGTTCTTCTCTCCACGAACGCACCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTg                                                                                              <  2:213619/140‑1 (MQ=255)                                             tGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGa                                                                                             >  1:186957/1‑140 (MQ=255)                                             tGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTCTATCGCCATTAAGGTGa                                                                                             >  1:552384/1‑140 (MQ=255)                                               aGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAg                                                                                           <  2:638115/140‑1 (MQ=255)                                                  ggCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGAtg                                                                                        <  2:606485/140‑1 (MQ=255)                                                   gCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGCCTCTCAGTCAATTGGATCGCCATTAAGGTGAAGAtgt                                                                                       >  1:231580/1‑140 (MQ=255)                                                          actCCGCCTGTCGGTTCTTCTGTCCCCGATCGCATCCTCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTa                                                                                <  1:214599/138‑1 (MQ=255)                                                            tCCGCCTGTCGGTTCTTCTGTCCTCGAACGCCTCCCCGATATTCACGCTAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACt                                                                              <  2:231580/140‑1 (MQ=255)                                                            tCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACt                                                                              <  2:539920/140‑1 (MQ=255)                                                            tCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACt                                                                              <  2:160212/140‑1 (MQ=255)                                                            tCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACt                                                                              <  2:186957/140‑1 (MQ=255)                                                            tCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACt                                                                              <  2:113042/140‑1 (MQ=255)                                                            tCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACt                                                                              <  1:635220/140‑1 (MQ=255)                                                            tCCGCCTGGCGGTTCTATTGTCCTCGAACGTGTGCACGAGATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACt                                                                              <  2:404202/140‑1 (MQ=255)                                                              cGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACTCg                                                                            >  2:662700/1‑140 (MQ=255)                                                              cGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACTCg                                                                            <  1:456605/140‑1 (MQ=255)                                                                   gTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACt                                                                              <  2:605728/133‑1 (MQ=255)                                                                   gTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACt                                                                              >  1:605728/1‑133 (MQ=255)                                                                    tCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGCCCCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACTCGCCCCa                                                                       <  2:654103/140‑1 (MQ=255)                                                                      ggTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACTCGCCCCAGaa                                                                    <  2:44754/140‑1 (MQ=255)                                                                             ctGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACTCGCCCCAGAACACCCtt                                                             <  1:158303/140‑1 (MQ=255)                                                                                  cTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACTCGCCCCAGAACACCCTTTTTta                                                        >  1:421580/1‑140 (MQ=255)                                                                                   tCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACTCGCCCCAGAACACCCTTTTTtat                                                       <  2:568654/140‑1 (MQ=255)                                                                                     gAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACTCGCCCCAGAACACCCTTTTTTATAc                                                     >  1:154188/1‑140 (MQ=255)                                                                                      aaCGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACTCGCCCCAGAACACCCTTTTTTATACg                                                    <  2:569900/140‑1 (MQ=255)                                                                                       aCGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACTCGCCCCAGAACACCCTTTTTTATACGa                                                   <  1:685480/140‑1 (MQ=255)                                                                                        cGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACTCGCCCCAGAACACCCTTTTTTATACGAc                                                  <  1:785958/140‑1 (MQ=255)                                                                                         gCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACTCGCCCCAGAACACCCTTTTTTATACGAct                                                 <  1:662700/140‑1 (MQ=255)                                                                                          cATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACTCGCCCCAGAACACCCTTTTTTATACGActa                                                <  1:86724/140‑1 (MQ=255)                                                                                                cgATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGTCAACTGTATCGCCATTGAGGTGAAGATGTTGTTTTACTCGCTCCAGATCTCCATTTTTTATACGActattctat                                          <  2:483756/140‑1 (MQ=255)                                                                                                cgATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACTCGCCCCAGAACACCCTTTTTTATACGActattctat                                          <  2:133809/140‑1 (MQ=255)                                                                                                                         gtTTATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACTCGCCCCAGAACACCCTTTTTTATGCGACTATTCTAGTTGTTTAACCGCTGGCGAAAAAAAc                 >  1:403953/1‑140 (MQ=255)                                                                                                                          tttATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACTCGCCCCAGAACACCCTTTTTTATACGACTATTCTATTTCTTTAACCGCTGGCGAAAAAAACt                <  2:421580/140‑1 (MQ=255)                                                                                                                          tttATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACTCGCCCCAGAACACCCTTTTTTATACGACTATTCTATTTCTTTAACCGCTGGCGAAAAAAACt                <  2:154188/140‑1 (MQ=255)                                                                                                                          tttATTTGCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACTCGCCCCAGAACACCCTTTTTTATACGACTATTCTATTTCTTTAACCGCTGGCGAAAAAAACt                <  2:94941/140‑1 (MQ=255)                                                                                                                                 gCAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACTCGCCCCAGAACACCCTTTTTTATACGACTATTCTATTTCTTTAACCGCTGGCGAAAAAAACTGACtata         <  1:645225/140‑1 (MQ=255)                                                                                                                                  cAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACTCGCCCCAGAACACCCTTTTTTCTACGACTATTCTATTTTTTTAACCGTTGGCGAAAAAAACTGACTATaa        >  2:338831/1‑140 (MQ=255)                                                                                                                                  cAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACTCGCCCCAGAACACCCTTTTTTATACGACTATTCTATTTCTTTAACCGCTGGCGAAAAAAAc                 >  2:702629/1‑131 (MQ=255)                                                                                                                                  cAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACTCGCCCCAGAACACCCTTTTTTATACGACTATTCTATTTCTTTAACCGCTGGCGAAAAAAAc                 <  1:702629/131‑1 (MQ=255)                                                                                                                                  cAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACTCGCCCCAGAACACCCTTTTTTATACGACTATTCTATTTCTTTAACCGCTGGCGAAAAAAACTGACTATaa        >  2:112994/1‑140 (MQ=255)                                                                                                                                  cAGGTGATTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACTCGCCCCAGAACACCCTTTTTTATACGACTATTCTATTTCTTTAACCGCTGGCGAAAAAAACTGACTATaa        >  1:633715/1‑140 (MQ=255)                                                                                                                                        aTTTGC‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACTCGCCCCAGAACACCCTTTTTTATACGACTATTCTATTTCTTTAACCGCTGGCGAAAAAAACTGACTATAAACTTCa  <  1:112994/140‑1 (MQ=255)                                                                                                                                             |                                                                                                                                        AATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCGCCGTGTTTATTTGCAGGTGATTTG‑‑CCCCCCTGCTGACTCTCAGCCAACTGTATCGCCATTAAGGTGAAGATGTTGTTTTACTCGCCCCAGAACACCCTTTTTTATACGACTATTCTATTTCTTTAACCGCTGGCGAAAAAAACTGACTATAAACTTCA  >  NC_000913/3836389‑3836662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑