| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,293,106 | (G)7→8 | coding (1595/1659 nt) | nrfE → | putative cytochrome c‑type biogenesis protein NrfE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,293,099 | 1 | . | G | 96.8% | 102.7 / ‑3.2 | 31 | coding (1588/1659 nt) | nrfE | putative cytochrome c‑type biogenesis protein NrfE |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (16/14); total (17/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
CACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGAT‑CGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑G‑GGGGGGAGGA‑TTGTTGATGATTGCG‑GGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCACGCTCAGGTCGTAGACACC > NC_000913/4292965‑4293231 | cACGCCGTCAACAAATGATGGATCCGTCAATACGCTGGAACGGCATCCCTGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGAT‑CGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑g‑gggg < 2:317334/140‑1 (MQ=255) cGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGAT‑CGTTACGCTTTTCGTCTGTATGTACAACGCGGTGTGCGCTGGATCT‑g‑gggggg > 1:321662/1‑140 (MQ=255) cGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGAT‑CGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑g‑gggggggag < 1:775452/140‑4 (MQ=255) atgGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGAT‑CGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGG‑GGGGGGAGGA‑TTGTtgatga > 1:663103/1‑140 (MQ=255) aaCCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGAT‑CGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGG‑GGGGGGAGGA‑TTGTTGATGATTGc > 1:18400/1‑140 (MQ=255) aaCCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGAT‑CGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGG‑GGGGGGAGGA‑TTGTTGATGATTGc > 1:691664/1‑140 (MQ=255) aCCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGAT‑CGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGG‑GGGGGGAGGA‑TTGTTGATGATTGc > 1:135178/1‑139 (MQ=255) aCCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGAT‑CGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGG‑GGGGGGAGGA‑TTGTTGATGATTGc < 2:135178/139‑1 (MQ=255) gCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGAT‑CGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGG‑GGGGGGAGGA‑TTGTTGATGATTGCG‑GGCGCATTGTTAAGCGGATg < 2:45472/140‑1 (MQ=255) tCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGAT‑CGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GAGGGGGGAGAA‑TTGTTGCTGATTGCG‑GGCGCATTGTTAAGCGGATGGCg > 2:210302/1‑140 (MQ=255) aCTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGAT‑CGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGG‑GGGGGGAGGA‑TTGTTGATGATTGCG‑GGCGCATTGTTAAGCGGATGGCGGGGgaa > 1:492047/1‑140 (MQ=255) tGGTATGCGGTCATGGGGGAG‑AAACTGGGCCGGGTCCGTAACGTTTTTCGTTGGT‑TGTACAAAGCCGGGGGGGGTGGGTCT‑T‑GGGGGGGGGAGTTGTTTAAGGTTTCGAGGCGCTTTCTTAAACGGATGCCggggaagga > 2:388520/1‑135 (MQ=255) ggTCATGGGGGAGAAAACTGGGCCGGAT‑CGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGG‑GGGGGGAGGA‑TTGTTGATGATTGCG‑GGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGAt < 1:597477/140‑1 (MQ=255) gTCATGGGGGAGAAAACTGGGCCGGAT‑CGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGG‑GGGGGGAGGA‑TTGTTGATGATTGCG‑GGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGAt > 2:479814/1‑139 (MQ=255) gTCATGGGGGAGAAAACTGGGCCGGAT‑CGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGG‑GGGGGGAGGA‑TTGTTGATGATTGCG‑GGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGAt < 1:479814/139‑1 (MQ=255) tCATGGGGGAGAAAACTGGGCCGGAT‑CGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGG‑GGGGGGAGGA‑TTGTTGATGATTGCG‑GGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGa > 1:793546/1‑140 (MQ=255) tGGGGGAGAAAACTGGGCCGGAT‑CGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGG‑GGGGGGAGGA‑TTGTTGATGATTGCG‑GGCGCATTGTTAAGCGGACGGGGGGGGATGAAGCGCGATGAATa > 1:393117/1‑140 (MQ=255) tGGGGGAGAAAACTGGGCCGGAT‑CGTTACGCATTTTGTTCGTATGTACAAAGCGGTGTGCGCTGGATCTGG‑GGGGGGAGGA‑TTGTTGATGATTGCG‑GGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATa < 2:275575/140‑1 (MQ=255) tGGGGGAGAAAACTGGGCCGGAT‑CGTTACACTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGG‑GGGGGGAGGA‑TTGTTGATGATTGCG‑GGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATa < 1:501445/140‑1 (MQ=255) ggAGAAAACTGGGCCGGAT‑CGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGG‑GGGGGGAGGA‑TTGTTGATGATTGCG‑GGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAgg < 2:663103/140‑1 (MQ=255) gagaAAACTGGGCCGGAA‑CGTTACGCTTTTCTTTTGTATCTACAAAGCGGTGTGCGCTGGATCTGG‑GGGGGGAGGA‑TTGTTGATGATTGCG‑GGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAggg < 2:321662/140‑1 (MQ=255) tGGGCCGGAT‑CGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGG‑GGGGGGAGGA‑TTGTTGATGATTGCG‑GGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCAc > 1:13348/1‑140 (MQ=255) ggCCGGAT‑CGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGG‑GGGGGGAGGA‑TTGTTGATGATTGCG‑GGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGc > 1:434485/1‑140 (MQ=255) ccGGAT‑CGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGG‑GGGGGGAGGA‑TTGTTGATGATTGCG‑GGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTa < 2:793546/140‑1 (MQ=255) gAT‑CGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGG‑GGGGGGAGGA‑TTGTTGATGATTGCG‑GGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCt < 2:555397/136‑1 (MQ=255) gAT‑CGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGG‑GGGGGGAGGA‑TTGTTGATGATTGCG‑GGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCt > 1:555397/1‑136 (MQ=255) ttCGTTTGTATGTACTAAGCGGAGTACGCTGGATCTGG‑GGGGGGAGGA‑TTGTTGATGATTGCG‑GGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCt < 1:388520/140‑1 (MQ=255) tgtaCAAAGCGGTGTGCGCTGGATCTGG‑GGGGGGAGGA‑TTGTTGATGATTGCG‑GGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCa > 2:507841/1‑140 (MQ=255) aCAAAGCGGTGTGCGCTGGATCTGG‑GGGGGGAGGA‑TTGTTGATGATTGCG‑GGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCACGc > 1:195827/1‑140 (MQ=255) aaaGCGGTGTGCGCTGGATCTGG‑GGGGGGAGGA‑TTGTTGATGATTGCG‑GGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCACGCTc < 2:18400/140‑1 (MQ=255) aaaGCGGTGTGCGCTGGATCTGG‑GGGGGGAGGA‑TTGTTGATGATTGCG‑GGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCACGCTc < 2:691664/140‑1 (MQ=255) gCGGTGTGCGCTGGATCTGG‑GGGGGGAGGA‑TTGTTGATGATTGCG‑GGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCACGCTCAgg < 2:195827/140‑1 (MQ=255) tgtgCGCTGGATCTGG‑GGGGGGAGGA‑TTGTTGATGATTGCG‑GGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCACGCTCAGGTCGt < 2:594602/140‑1 (MQ=255) tgtgCGCTGGATCTGG‑GGGGGGAGGA‑TTGTTGATGATTGCG‑GGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCACGCTCAGGTCGt > 1:637228/1‑140 (MQ=255) gcTGGATCTGG‑GGGGGGAGGA‑TTGTTGATGATTGCG‑GGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCCCGCTACTGCTGTAATTAACCTGTTTTGCGCACGCTCAGGTCGTAGaca > 2:281246/1‑140 (MQ=255) tGGATCTGG‑GGGGGGAGGA‑TTGTTGATGATTGCG‑GGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCACGCTCAGGTCGTAGACAcc > 2:451123/1‑140 (MQ=255) | CACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGAT‑CGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑G‑GGGGGGAGGA‑TTGTTGATGATTGCG‑GGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCACGCTCAGGTCGTAGACACC > NC_000913/4292965‑4293231 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |