Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,492,583	(G)6→7	intergenic (‑58/+4)	idnT ← / ← idnO	L‑idonate/5‑ketogluconate/gluconate transporter/5‑keto‑D‑gluconate 5‑reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,492,577	1	.	G	100.0%	96.2 / NA	29	intergenic (‑52/+10)	idnT/idnO	L‑idonate/5‑ketogluconate/gluconate transporter/5‑keto‑D‑gluconate 5‑reductase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (17/12);  total (17/12)

TAAAGCCGTTAACTTTAAAGCCGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTA‑GGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGC  >  NC_000913/4492450‑4492702                                                                                                                                 |                                                                                                                              tAAAGCCGTTAACTTTAAAGCCGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGGCTGtt                                                                                                                    >  1:60091/1‑140 (MQ=255)    aGCCTTTAACTTTAAAGCCGATCATCAGGATAAGAAGCAGCGCGTCGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGGCTGTTaaa                                                                                                                 <  2:707485/140‑1 (MQ=255)    aGCCGTTAACTTTAAAGCCGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGGCTGTaaaa                                                                                                                 <  1:438660/140‑1 (MQ=255)    aGCCGTTAACTTTAAAGCCGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGGCTGTTaaa                                                                                                                 <  2:60091/140‑1 (MQ=255)      ccGTTAACTTTAAAGCCGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGGCTGTTaaa                                                                                                                 >  2:697346/1‑138 (MQ=255)      ccGTTAACTTTAAAGCCGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGGCTGTTaaa                                                                                                                 <  1:697346/138‑1 (MQ=255)      ccGTTAACTTTAAAGCCGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTA‑ggggg                                                                                                                          <  1:361307/128‑1 (MQ=255)      ccGTTAACTTTAAAGCCGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTCCCAGAAAAGCAGCGCTTTCGCGCTGCACGTA‑ggggg                                                                                                                          >  2:361307/1‑128 (MQ=255)       cGTTAACTTTAAAGCCGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGGCTGTTAAACAg                                                                                                              >  1:458146/1‑140 (MQ=255)             cTTTAAAGCCGATCATCAGAATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGGCTGTTAAACAGCCACTa                                                                                                        <  2:741742/140‑1 (MQ=255)                        atcatcAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGGCTGTTAAACAGCCACTAACATGCCGCCa                                                                                             >  1:28045/1‑140 (MQ=255)                        atcatcAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGGCTGTTAAACAGCCACTAACATGCCGCCa                                                                                             >  2:493306/1‑140 (MQ=255)                           atcaGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGGCTGTTAAACAGCCACTAACATGCCACCATca                                                                                          <  2:458146/140‑1 (MQ=255)                                  tAAGAAGCAGCGCGATGCCTGCAGCGATATTGATTAAGGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCaacaaaca                                                                                   <  1:385917/140‑1 (MQ=255)                                          agcGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTgg                                                                           >  2:644282/1‑140 (MQ=255)                                          agcGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGCgg                                                                           >  1:75307/1‑140 (MQ=255)                                                                       tGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTgaaga                                              <  2:542060/140‑1 (MQ=255)                                                                                 tttATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGAGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACAcc                                    >  1:697713/1‑140 (MQ=255)                                                                                   tATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCgc                                  >  1:551538/1‑140 (MQ=255)                                                                                    aTCTCTTATTAGTGCCCTAAAAACAGCGCTTTCTCCCTGGACGTAGGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCgca                                 <  2:403623/140‑1 (MQ=255)                                                                                         ttattaGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTTGCCGTTTATGAATTCAGAGGCTTTTTAAGAAAGGAACACCGCAGCAcc                            >  2:299256/1‑140 (MQ=255)                                                                                         ttattaGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCAcc                            >  1:822565/1‑140 (MQ=255)                                                                                         ttattaGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAAGCTGTTGAAGAAATGAACACCGCAGCAcc                            >  1:386191/1‑140 (MQ=255)                                                                                          tattaGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTgaagaa                                             <  2:615094/122‑1 (MQ=255)                                                                                          tattaGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTgaagaa                                             >  1:615094/1‑122 (MQ=255)                                                                                             taGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATc                        <  1:493306/140‑1 (MQ=255)                                                                                               gTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAAt                         >  2:137182/1‑137 (MQ=255)                                                                                               gTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAAt                         <  1:137182/137‑1 (MQ=255)                                                                                                tGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCGGAGACTTTTGAAGAAAAGAACACCGCAGCACCAATCAGt                     >  2:301423/1‑140 (MQ=255)                                                                                                   cAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTcc                  >  2:642975/1‑140 (MQ=255)                                                                                                                   tCGCGCTGCACGTAGGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCAGCGGATCTCCCCAGc  >  1:368523/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                              TAAAGCCGTTAACTTTAAAGCCGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTA‑GGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGC  >  NC_000913/4492450‑4492702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑