| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,048,161 | (G)5→6 | coding (531/747 nt) | gfcC ← | capsule biosynthesis GfcC family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,048,156 | 1 | . | G | 97.9% | 160.4 / ‑3.6 | 47 | coding (536/747 nt) | gfcC | capsule biosynthesis GfcC family protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (20/26); total (20/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGAAAAGCCCAACCAAAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑C‑GGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCACCCAGCAGAGTAATAGTCACCGGACGCTGTACGGTATAGAGCGTATAGTCCCCGAC > NC_000913/1048023‑1048286 | aGAAAAGCCCAACCAACGCTGGCTGCCAGGAGGTGCTTCTACATGTCTATTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑C‑gggggg < 2:375649/140‑2 (MQ=255) aaaaGCCCAACCAAAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑C‑ggggggtg < 2:20567/140‑4 (MQ=255) cccAACCAAAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCtt > 2:729919/1‑140 (MQ=255) accaaAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAg < 1:428070/140‑1 (MQ=255) ccaaAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCGACAGGGCAACAGGGGCGACGACAGTCTCGCCGTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGa < 2:164462/140‑1 (MQ=255) ccaaAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCTCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGa < 2:782481/140‑1 (MQ=255) ccaaAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCCAGG‑CGGGGGGTGATCTTGCAGa > 2:336986/1‑140 (MQ=255) ccaaAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCCTGCAGa > 2:783997/1‑140 (MQ=255) ggctgCCAGGCGGTGGTTCCACCTGTCTTTTCTCCTACAGGGCAACAGTGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGt < 2:363908/140‑1 (MQ=255) ggctgCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGt > 1:147475/1‑140 (MQ=255) ggtTCCACATGTCTTTTATTCCACACGGCCACAGGGGGGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGgcctg < 1:314789/140‑1 (MQ=255) tCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGgcctgcct < 2:609538/140‑1 (MQ=255) tGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCctgcctgc < 1:166491/136‑1 (MQ=255) tGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCctgcctgc > 2:166491/1‑136 (MQ=255) ggCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCa > 1:133375/1‑140 (MQ=255) ggCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCa > 2:564018/1‑140 (MQ=255) agCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCa > 1:8160/2‑140 (MQ=255) cAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCAGCCCCAGa > 2:535478/1‑140 (MQ=255) aGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAAcc > 2:817174/1‑140 (MQ=255) ggCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCa < 1:660612/140‑1 (MQ=255) cgacgaCAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCAcc < 1:92703/140‑1 (MQ=255) cgacgaCAGTCTCACACTCAGGCGTCATCGCCATAACCTTATTCTTGTCCGTGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCAcc < 1:300518/140‑1 (MQ=255) acgacAGTCTCACCCTCAGGTGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCAcc > 1:133255/1‑138 (MQ=255) acgacAGTCTCACCCTCAGGTGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCAcc < 2:133255/138‑1 (MQ=255) cgacAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCACCcag < 1:729919/140‑1 (MQ=255) cgacAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCACCcag < 1:817174/140‑1 (MQ=255) agacgggctcGCCCTCAGGCGTAATCAGCATAACGGTATCCGTGTCCGTGCCTGCAAGG‑CGGGGGGTGATCCTGCAGATAATCGGTAACGCTGCGGCCTGCCTGCCGGGGCAATTGCCCGGGCCCAGAATTCGCACCcag < 2:334972/133‑1 (MQ=255) acAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCACCcagca < 2:147475/140‑1 (MQ=255) gTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTACGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCACCCAGCagag > 1:535739/1‑140 (MQ=255) aGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGAGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGACAATTGCCCGGCCCCAGAAACCGCACCCAGCAGAGTAATAGTCAcc > 1:561477/1‑140 (MQ=255) gCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCAGCCCCAGAAACCGCACCCAGCAGAGTAATAGTCACCgg < 1:535478/140‑1 (MQ=255) cGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCACCCAGCAGAGTAATAGTCACCGGa < 1:529383/140‑1 (MQ=255) aTCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCACCCAGCAGAGTAATAGTCACCGGACGCt < 2:8160/140‑1 (MQ=255) aTCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCACCCAGCAGAGTAATAGTCACCGGACGCt < 1:490072/140‑1 (MQ=255) aTCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCACCCAGCAGAGTAATAGTCACCGGACGCt < 1:440998/140‑1 (MQ=255) aTCACCATAACGTTACTCTTGTTCGAGCCTGCAAGGCC‑GGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCACCCAGCAGAGTAATAGTCACCGGACGCt < 1:336986/140‑1 (MQ=255) cACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCACCCAGCAGAGTAATAGTCACCGGACGCTGt < 1:495700/140‑1 (MQ=255) cATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCACCCAGCAGAGTAATAGTCACCGGACGCTGTa > 2:408434/1‑138 (MQ=255) cATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCACCCAGCAGAGTAATAGTCACCGGACGCTGTa < 1:408434/138‑1 (MQ=255) cATAACGTTATTCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCACCCAGCAGAGTAATAGTCACCGGACGCTGTACg > 1:406195/1‑140 (MQ=255) ttCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAGTTGCCCGGCCCCAGAAGCCGCACCCGGCAGAGTGATAGTCACTGGACACTGTACGGTATAGAGCg > 2:214743/1‑140 (MQ=255) ttCTTGTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCACCCAGCAGAGTAATAGTCACCGGACGCTGTACGGTATAGAGCg > 1:465720/1‑140 (MQ=255) cTTGTACGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCACCCAGCAGAGTAATAGTCACCGGACGCTGTATGGTATAGAGCGta < 2:535739/140‑1 (MQ=255) gTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCACCCAGCAGAGTAATAGTCACCGGACGCTGt < 2:43455/122‑1 (MQ=255) gTCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCACCCAGCAGAGTAATAGTCACCGGACGCTGt > 1:43455/1‑122 (MQ=255) tCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCACCCAGCAGAGTAATAGTCACCGGACGCTGTACg > 1:78839/1‑124 (MQ=255) tCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCACCCAGCAGAGTAATAGTCACCGGACGCTGTACg < 2:78839/124‑1 (MQ=255) tCCGCGCCTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCACCCAGCAGAGTAATAGTCACCGGACGCTGTACGGTATAGAGCGTATAGt > 2:579854/1‑140 (MQ=255) ccGCGCCTGCAAGG‑CGGGGGGTGATCCTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCACCCAGCAGAGTAATAGTCACCGGACGCTGTACGGTATAGAGCGTATAGTc < 1:783997/140‑1 (MQ=255) cTGCAAGG‑CGGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCACCCAGCAGAGTAATAGTCACCGGACGCTGTACGGTATAGAGCGTATAGTCCCCGAc < 2:406195/140‑1 (MQ=255) | AGAAAAGCCCAACCAAAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGG‑C‑GGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCACCCAGCAGAGTAATAGTCACCGGACGCTGTACGGTATAGAGCGTATAGTCCCCGAC > NC_000913/1048023‑1048286 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |