| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,066,854 | (G)5→6 | intergenic (+28/+10) | agp → / ← yccJ | glucose‑1‑phosphatase/PF13993 family protein YccJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,066,849 | 1 | . | G | 94.1% | 108.4 / 0.2 | 34 | intergenic (+23/+15) | agp/yccJ | glucose‑1‑phosphatase/PF13993 family protein YccJ |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (14/18); total (15/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01 | |||||||||||
ACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGC‑GGGGG‑AGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCTTCTTCCCAAATCTTTTCCGCCATTT > NC_000913/1066710‑1066982 | aCCCTGCAGGCACCTCCGCAGCTTGGGACCCTGGAATCAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGc < 1:211229/140‑1 (MQ=255) cTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGC‑ggg > 1:594472/1‑140 (MQ=255) tGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGC‑gggg > 1:344460/1‑140 (MQ=255) gCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGC‑ggggg > 2:629753/1‑140 (MQ=255) gCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGC‑ggggg < 2:203494/140‑1 (MQ=255) gCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCCGTGAAATAACATAAAACTCCCGCGCGGGAAGC‑ggggg > 1:258874/1‑140 (MQ=255) aCCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGG‑AGTCg < 1:797854/140‑1 (MQ=255) aCCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGG‑AGTCg < 1:422084/140‑1 (MQ=255) cgcAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGG‑AGTCGCTGGt > 2:434728/1‑140 (MQ=255) aGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGG‑AGTCGCTGGTTaa < 2:37265/140‑1 (MQ=255) aGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGG‑AGTCGCTGGTTaa < 1:582017/140‑1 (MQ=255) aaatAAGCGGTTGCCCGGTAGAAGCCGATGGTTTCTGCCTGATGGAGAAGTTTGTTAGCGGGTTGAATGAAGCGGGGGAATAACAGAAAACTCCCCCGCGGGAAGCGGGGGG‑AGTCGCTGGTTAAACGTTTTTACGTTCg < 2:258874/137‑1 (MQ=255) ttAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGG‑AGTCGCTGGTTAAACGTTTTTACGTTCGAt > 2:410728/1‑140 (MQ=255) ttGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGG‑AGTCGCTGGTTAAACGTTTTTAc < 1:479867/125‑1 (MQ=255) ttGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGG‑AGTCGCTGGTTAAACGTTTTTAc > 2:479867/1‑125 (MQ=255) gCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGG‑AGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCg > 2:129568/1‑140 (MQ=255) gCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGG‑AGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAg > 1:168992/1‑140 (MQ=255) cTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGG‑AGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGc < 1:605851/140‑1 (MQ=255) tGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGG‑AGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAAt < 2:97502/140‑1 (MQ=255) tGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGG‑AGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAAt > 2:170824/1‑140 (MQ=255) tGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGG‑AGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAAt < 1:129568/140‑1 (MQ=255) tCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGG‑AGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTa < 1:170824/140‑1 (MQ=255) tCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGG‑AGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTa < 1:410728/140‑1 (MQ=255) cTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGG‑AGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTAt < 2:727081/140‑1 (MQ=255) cGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGG‑AGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTc < 2:344460/140‑1 (MQ=255) gATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGG‑AGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCt > 1:201277/1‑140 (MQ=255) aTGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGC‑GGGGGAAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCtt > 1:827200/1‑140 (MQ=255) aTAAGTTTGATAGCGGGTTGAATGAAGCGTTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGG‑AGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCa < 1:295108/140‑1 (MQ=255) ttGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGG‑AGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCt < 1:434728/140‑1 (MQ=255) tAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGG‑AGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGa > 2:459446/1‑140 (MQ=255) gCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGG‑AGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACt > 2:44873/1‑140 (MQ=255) ttGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGG‑AGTCGCTGGTTAAACGTTTTTACGTTAGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAacac < 1:570133/140‑1 (MQ=255) atgaaGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGG‑AGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTtca < 1:629753/140‑1 (MQ=255) atgaaGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGC‑GGGGGAAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTtca < 2:827200/140‑1 (MQ=255) gCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGG‑AGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACt < 1:459446/140‑1 (MQ=255) gCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGG‑AGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACt > 2:791623/1‑140 (MQ=255) cgcgAGAAGCGGGGGG‑AGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCTTCTTCCCAAATCTTTTCCGcc > 2:535798/1‑140 (MQ=255) cgAGAAGCGGGGGG‑AGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTAGCTTCTTCCCTAATCTTTTCCGCCAt > 1:337520/1‑140 (MQ=255) cgAGAAGCGGGGGG‑AGGCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCTTCTTCCCAAATCTTTTCCGCCAt > 1:24726/1‑140 (MQ=255) agaAGCGGGGGG‑AGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCTTCTTCCCAAATCTTTTCCGCCAttt > 1:756805/1‑140 (MQ=255) | ACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGC‑GGGGG‑AGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCTTCTTCCCAAATCTTTTCCGCCATTT > NC_000913/1066710‑1066982 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |