Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	2,857,096	Δ2,538 bp	coding (4‑2541/2562 nt)	mutS →	DNA mismatch repair protein MutS

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	2857096	2859633	2538	61 [0]	[0] 60	mutS	DNA mismatch repair protein MutS

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2857095	0 (0.000)	60 (1.220)	37/276	0.2	100%	coding (3/2562 nt)	mutS	DNA mismatch repair protein MutS
	?	NC_000913	2859634 =	0 (0.000)					coding (2542/2562 nt)	mutS	DNA mismatch repair protein MutS

GGAACCAGCATCAAGAACTCGCCGTTCGCTTCTTCCCCTGAAATGATTAACTCCGGTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTATCTCAGGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGT  >  NC_000913/2856956‑2857234                                                                                                                                            |                                                                                                                                            ggAACCAGCATCAAGAACTCGCCGTTCGCTTCTTCCCCTGAAATGATTAACTCCGGTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATACCCCCATg                                                                                                                                             <  2:569830/140‑1 (MQ=255)        gCATCAAGAACTCGCCGTTCGCTTCTTCCCCTGAAATGATTAACTCCGGTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttga                                                                                                                                      <  1:359952‑M1/140‑8 (MQ=255)        gCATCAAGAACTCGCCGTTCGCTTCTTCCCCAGAAATGATTAACTCCGGTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttga                                                                                                                                      <  2:321590‑M1/140‑8 (MQ=255)         cATCAAGAACTCGCCGTTCGCTTCTTCCCCTGAAATGATTAACTCCGGTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaa                                                                                                                                     >  1:120398‑M1/1‑132 (MQ=255)         cATCAAGAACTCGCCGTTCGCTTCTTCCCCTGAAATGATTAACTCCGGTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaa                                                                                                                                     <  2:714967‑M1/140‑9 (MQ=255)         cATCAAGAACTCGCCGTTCGCTTCTTCCCCTGAAATGATTAACTCCGGTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaa                                                                                                                                     <  2:445512‑M1/140‑9 (MQ=255)              aGAACTCGCCGTTCGCTTCTTCCCCTGAAATGATTAACTCCGGTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcc                                                                                                                                <  2:492654‑M1/140‑14 (MQ=255)              aGAACTCGCCGTTCGCTTCTTCCCCTGAAATGATTAACTCCGGTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcc                                                                                                                                <  1:734898‑M1/140‑14 (MQ=255)              aGAACTCGCCGTTCGCTTCTTCCCCTGAAATGATTAACTCCGGTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcc                                                                                                                                <  2:656902‑M1/140‑14 (MQ=255)              aGAACTCGCCGTTCGCTTCTTCCCCTGAAATGATTAACTCCGGTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcc                                                                                                                                <  2:669902‑M1/140‑14 (MQ=255)               gAACTCGCCGTTCGCTTCTTCCTCTGAAATGATTATCTCCGGTATCATCTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcct                                                                                                                               <  2:270251‑M1/140‑15 (MQ=255)               gAACTCGCCGTTCGCTTCTTCCCCTGAAATGATTAACTCCGGTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcct                                                                                                                               <  1:734837‑M1/140‑15 (MQ=255)                 aCTCGCCGTTCGCTTCTTCCCCTGAAATGATTAACTCCGGTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctgg                                                                                                                             >  1:635153‑M1/1‑124 (MQ=255)                   tCGCCGTTCGCTTCTTCCCCTGAAATGATTAACTCCGGTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGAAACCGGAAATAACCGCATGcggttgaagagcctggtg                                                                                                                           >  1:270251‑M1/1‑122 (MQ=255)                       cGTTCGCTTCTTCCCCTGAAATGATTAACTCCGGTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaat                                                                                                                       <  1:675293‑M1/140‑23 (MQ=255)                         ttCGCTTCTTCCCCTGAAATGATTAACTCCGGTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataa                                                                                                                     >  2:551372‑M1/1‑116 (MQ=255)                                   ccccTGAAATGATTAACTCCGGTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccga                                                                                                           <  2:635153‑M1/140‑35 (MQ=255)                                               ttAACTCCGGTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgct                                                                                               <  2:120398‑M1/140‑47 (MQ=255)                                                tAACTCCGGTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgcta                                                                                              >  1:95059‑M1/1‑93 (MQ=255)                                                 aaCTCCGGTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctat                                                                                             >  1:446039‑M1/1‑92 (MQ=255)                                                   cTCCGGTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcg                                                                                           >  2:648621‑M1/1‑90 (MQ=255)                                                        gTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaat                                                                                      >  1:520145‑M1/1‑85 (MQ=255)                                                        gTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaat                                                                                      >  1:425052‑M1/1‑85 (MQ=255)                                                        gTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaat                                                                                      >  1:326401‑M1/1‑85 (MQ=255)                                                        gTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgct                                                                                               >  1:121868‑M1/1‑85 (MQ=255)                                                        gTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgct                                                                                               <  2:121868‑M1/131‑47 (MQ=255)                                                         tATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaata                                                                                     <  1:141268‑M1/140‑57 (MQ=255)                                                                tgCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacga                                                                              >  2:108021‑M1/1‑77 (MQ=255)                                                                 gcgcCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgac                                                                             <  2:446039‑M1/140‑65 (MQ=255)                                                                 gcgcCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgac                                                                             <  1:108021‑M1/140‑65 (MQ=255)                                                                     cTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaact                                                                         >  2:186240‑M1/1‑72 (MQ=255)                                                                     cTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaact                                                                         >  2:125321‑M1/1‑72 (MQ=255)                                                                           tgATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaa                                                                   <  1:160564‑M1/140‑75 (MQ=255)                                                                            gATTACAACGAAAATAAACACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaat                                                                  <  2:758094‑M1/140‑76 (MQ=255)                                                                                      agaataAAAACCATCCCACCCCGTTAAATCTCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaaaa                                                        <  1:393332‑M1/138‑86 (MQ=255)                                                                                        agacagaaccatcaCCCCCCATGTAATATTAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaaaaca                                                      <  1:246756‑M1/134‑88 (MQ=255)                                                                                          tAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaaaacacc                                                    <  1:9274‑M1/140‑90 (MQ=255)                                                                                          tAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaaaacacc                                                    <  2:520145‑M1/140‑90 (MQ=255)                                                                                          tAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaaaacacc                                                    <  1:696313‑M1/140‑90 (MQ=255)                                                                                          tAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaaaacacc                                                    <  2:425052‑M1/140‑90 (MQ=255)                                                                                                   tCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattgacggcgactgacgaataaaatacaaacaccatgtatact                                           >  2:393332‑M1/1‑42 (MQ=255)                                                                                                   tCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaaaaca                                                      >  2:606250‑M1/1‑42 (MQ=255)                                                                                                   tCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaaaaca                                                      <  1:606250‑M1/129‑88 (MQ=255)                                                                                                    cacaCCCCATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactggcgagtaaaataaaaacaccctgtataata                                          >  1:256774‑M1/1‑41 (MQ=255)                                                                                                         cccATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatgttaacgacaactgacgcataaaatacaaacaccctgtataatctgtct                                     >  2:246756‑M1/1‑36 (MQ=255)                                                                                                         cccATTTAATATCAGGGAACCGAACATAAGCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaaaacaccctgtataataggaaa                                     <  1:374972‑M1/140‑105 (MQ=255)                                                                                                         cccATTTAATATCAGGGAACCGAACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaaaacaccctgtataataggaaa                                     <  2:326401‑M1/140‑105 (MQ=255)                                                                                                           cATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaaaacacc                                                    <  2:672631‑M1/123‑90 (MQ=255)                                                                                                           cATTTAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaaaacacc                                                    >  1:672631‑M1/1‑34 (MQ=255)                                                                                                             tttAATATCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaaaacaccctgtataataggaaagctt                                 >  2:94461‑M1/1‑32 (MQ=255)                                                                                                                   atCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaaaacaccctgtataataggaaagcttatttta                           <  2:95059‑M1/140‑115 (MQ=255)                                                                                                                   atCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaaaacaccctgtataataggaaagcttatttta                           >  1:598286‑M1/1‑26 (MQ=255)                                                                                                                   atCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaaaacaccctgtataataggaaagcttatttta                           >  1:475503‑M1/1‑26 (MQ=255)                                                                                                                   atCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaaaacaccctgtataataggaaagcttatttta                           <  2:598286‑M1/140‑115 (MQ=255)                                                                                                                   atCAGGGAACCGGACATAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaaaacaccctgtataataggaaagcttatttta                           <  1:94461‑M1/140‑115 (MQ=255)                                                                                                                                  aTAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaaaacaccctgtataataggaaagcttattttacagggtaaaaccatg            <  1:648621‑M1/140‑130 (MQ=255)                                                                                                                                  aTAACCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaaaacaccctgtataataggaaagcttattttacagggtaaaaccatg            <  1:125321‑M1/140‑130 (MQ=255)                                                                                                                                    aaCCCCATGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaaaacaccctatataataggaaagcttattttacagggtaaaaccatgcc          >  2:292132‑M1/1‑9 (MQ=255)                                                                                                                                           tGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaaaacaccctgtataataggaaagcttattttacagggtaaaaccatgccatctaca   <  1:551372‑M1/140‑139 (MQ=255)                                                                                                                                           tGcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaaaacaccatgtataataggaaagcgtattttacggggaaaaaccatgccatctaca   >  2:244381‑M1/1‑2 (MQ=255)                                                                                                                                            gcgcttgaagagcctggtgtaataacaattcccgatagtcttttgctatcgggaatattaacgacaactgacgaataaaataaaaacaccctgtataataggaaagcttattttacagggtaaaaccatgccatctacac  >  1:490135‑M1/1‑1 (MQ=255)                                                                                                                                            |                                                                                                                                            GGAACCAGCATCAAGAACTCGCCGTTCGCTTCTTCCCCTGAAATGATTAACTCCGGTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTATCTCAGGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGT  >  NC_000913/2856956‑2857234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑