Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	8,964	A→G	T243A (ACC→GCC)	talB →	transaldolase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	8,964	0	A	G	98.1%	180.0 / NA	53	T243A (ACC→GCC)	talB	transaldolase B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (27/25);  minor base C (0/1);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGG  >  NC_000913/8827‑9099                                                                                                                                          |                                                                                                                                        cGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTTTGGAACTGGCAGGCTGCGACCGTCTGGcc                                                                                                                                       >  1:372122/1‑140 (MQ=255) cGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCATCTGGcc                                                                                                                                       >  1:631685/1‑140 (MQ=255)  ggCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCa                                                                                                                                      >  1:104965/1‑140 (MQ=255)           tCCGGGCGTGGTTTCTGAATCTTAAATCTACTAGTACTATAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCgg                                                                                                                             <  2:208787/140‑1 (MQ=255)             cGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCa                                                                                                                           <  2:346471/140‑1 (MQ=255)               ggCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCgg                                                                                                                             >  2:367096/1‑136 (MQ=255)               ggCGTGGTTTCTGTATCTGAAATCTACCAGTACGACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCgg                                                                                                                             <  1:367096/136‑1 (MQ=255)                   tGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCActgctg                                                                                                                     >  2:523193/1‑140 (MQ=255)                   tGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCActgctg                                                                                                                     >  2:39130/1‑140 (MQ=255)                   tGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTAGCAGGCTTAGACCGTCTGGCCGGCGCACCGCCActgctg                                                                                                                     >  2:400529/1‑140 (MQ=255)                     gTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCACTGCTGaa                                                                                                                   >  2:823500/1‑140 (MQ=255)                     gTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCACTGCTGaa                                                                                                                   <  2:585931/140‑1 (MQ=255)                         cTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGTACTGCTGAAagag                                                                                                               <  1:823500/140‑1 (MQ=255)                               cTGAAATCTACCAGTTCTACAAAGATCACGGTTATGACACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCg                                                                                                         <  1:400529/140‑1 (MQ=255)                               cTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCa                                                                                                                           >  1:115466/1‑122 (MQ=255)                               cTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCa                                                                                                                           <  2:115466/122‑1 (MQ=255)                               cTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCg                                                                                                         >  2:62157/1‑140 (MQ=255)                               cTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCg                                                                                                         <  1:523193/140‑1 (MQ=255)                                       tACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGa                                                                                                 <  2:641815/140‑1 (MQ=255)                                        aCCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGaa                                                                                                <  2:454762/140‑1 (MQ=255)                                            gTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAgggg                                                                                            <  1:62157/140‑1 (MQ=255)                                               ctacAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCACTGCTGAACGATCTGGCGCAGAGCGAAGGGGCTa                                                                                         >  1:384945/1‑140 (MQ=255)                                               ctacAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTa                                                                                         >  2:690203/1‑140 (MQ=255)                                                tacAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCATCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTAt                                                                                        <  2:631685/140‑1 (MQ=255)                                                       agCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGt                                                                                 >  1:698119/1‑140 (MQ=255)                                                       agCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGt                                                                                 >  1:728611/1‑140 (MQ=255)                                                       agCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGt                                                                                 >  1:778859/1‑140 (MQ=255)                                                       agCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGt                                                                                 >  1:126101/1‑140 (MQ=255)                                                       agCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGt                                                                                 >  2:544021/1‑140 (MQ=255)                                                       agCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGt                                                                                 >  2:453481/1‑140 (MQ=255)                                                         cACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGATGGCGGAGCGCGAAGGGGCTATCGAACGTaa                                                                               >  2:304749/1‑140 (MQ=255)                                                          aCGGTTATGAAACCGTTTTTATTGGCGCAAGCTTCTGTACCATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTaaa                                                                              <  2:372122/140‑1 (MQ=255)                                                          aCGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTaaa                                                                              >  2:422044/1‑140 (MQ=255)                                                          aCGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTaaa                                                                              <  1:690203/140‑1 (MQ=255)                                                                      ccGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACAcc                                                                  >  1:669392/1‑140 (MQ=255)                                                                          ggTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCg                                                              <  2:778859/140‑1 (MQ=255)                                                                              aTGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGt                                                          >  2:13376/1‑140 (MQ=255)                                                                                         cTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTc                                               <  2:104965/140‑1 (MQ=255)                                                                                           tCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCg                                             <  2:457448/140‑1 (MQ=255)                                                                                             cGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGgc                                           <  2:707630/140‑1 (MQ=255)                                                                                                   aTCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACTGGCCAATTGAAAGCGCGTCCGGCGCGTAt                                     >  1:315878/1‑140 (MQ=255)                                                                                                               cTGGAACTGGCAGGCTGCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGa                         <  2:649640/140‑1 (MQ=255)                                                                                                                       ggCAGGCTGCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAGGCGCGTCGGGCGCGGATCACTAAGTCCGAGTTCCtgt                 >  1:222849/1‑140 (MQ=255)                                                                                                                         cAGGCTGCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTgg               <  2:125194/140‑1 (MQ=255)                                                                                                                         cAGGCTGCGACCGTCTGCCCATCGCACCGGCTCTGCTGATAGAGCTGGTGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTgg               <  2:222849/140‑1 (MQ=255)                                                                                                                          aGGCTGCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGgc              >  1:550714/1‑140 (MQ=255)                                                                                                                            gCTGCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGgcag            <  2:550714/140‑1 (MQ=255)                                                                                                                               gCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGACAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGcac         <  2:499962/140‑1 (MQ=255)                                                                                                                               gCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGcac         <  1:500750/140‑1 (MQ=255)                                                                                                                               gCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGcac         >  1:499962/1‑140 (MQ=255)                                                                                                                               gCGACCGTCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGcac         <  1:453481/140‑1 (MQ=255)                                                                                                                                     gTCTGGCCATCGCACCGGCACTCCTGAAAGAACTGACGGTGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAg   <  2:315878/140‑1 (MQ=255)                                                                                                                                      tCTGGCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAgg  >  2:540418/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        CGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGG  >  NC_000913/8827‑9099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑