| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,361,393 | C→T | R92C (CGT→TGT) | puuD → | gamma‑glutamyl‑gamma‑aminobutyrate hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,361,393 | 0 | C | T | 97.0% | 101.6 / NA | 33 | R92C (CGT→TGT) | puuD | gamma‑glutamyl‑gamma‑aminobutyrate hydrolase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (17/15); minor base A (0/1); total (17/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGCCGCACCTATATGGTGAAAACGGCGATGAGCCTGACGCCGATCCCGGGCGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGTTCGTTGCATCGCAAGCTGTGCGAACAGCCTGA > NC_000913/1361257‑1361529 | acaTGCGCTGGCGGAACCGTCATTACTTGAATAACTTTTACCGAAACTGGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGCCGCACCTATATGGTGAAAACGGCGATGAGCCTGACGCCGATCCCGGGtgtg < 2:342457/140‑1 (MQ=255)acaTGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGCCGCACCTATATGGTGAAAATGGCGATGAGCCTGACGCCGATCCCGGGtgtg < 2:790716/140‑1 (MQ=255)acaTGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGCCGCACCTATATGGTGAAAACGGCGATGAGCCTGACGCCGATCCCGGGtgtg < 1:196429/140‑1 (MQ=255)acaTGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGCCGCACCTATATGGTGAAAACGGCGATGAGCCTGACGCCGATCCCGGGtgtg < 1:810097/140‑1 (MQ=255) tGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGCCGCACCTATATGGTGAAAACGGCGATGAGCCTGACGCCGATCCCGGGTGTGATCTTCTg > 1:492290/1‑140 (MQ=255) gCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGCCGCACCTATATGGTGAAAACGCCGATGAGGCTGACGCCTATCCAGGGTGTGATCTTCTGAg > 2:259445/1‑140 (MQ=255) aaCCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGCCGCACCTATATGGTGAAAACGGCGATGAGCCTGACGCCGATCCCGGGTGTGATCTTCTGAGCATg < 2:492290/140‑1 (MQ=255) cGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGCCGCACCTATATGGTGAAAACGGCGATGAGCCTGACGCCGATCCCGGGTGTGATCTTCTGAGCATGGCg < 2:569041/140‑1 (MQ=255) cGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGCCGCACCTATATGGTGAAAACGGCGATGAGCCTGACGCCGATCCCGGGTGTGATCTTCTGAGCATGGCg < 1:598430/140‑1 (MQ=255) gCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGCCGCACCTATATGGTGAAAACGGCGATGAGCCTGACGCCGATCCCGGGTGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGaaa > 1:581883/1‑140 (MQ=255) ggTAGTCCCAGCAATGTGCAGCCGCACCTATATGGTGAAAACGGCGATGAGCCTGACGCCGATCCCGGGTGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCg < 1:473057/140‑1 (MQ=255) gTCCCAGCAATGTGCAGCCGCACCTTTATTGTGAAAAGGGCGATGAGCCTGACGCCGATCCCGGGTGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGt < 1:259445/140‑1 (MQ=255) tCCCAGCAATGTGCAGCCGCACCTATATGGTGAAAACGGCGATGAGCCTGACGCCGATCCCGGGTGTGATCTTCTGAGCATGGCTATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGtt > 2:775237/1‑140 (MQ=255) ccAGCAATGTGCAGCCGCACCTATATGGTGAAAACGGCGATGAGCCTGACGCCGATCCCGGGTGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTa > 2:643949/1‑140 (MQ=255) aaTGTGCAGCCGCACCTATATGCTGAAAACGGCGATGAGCCTGACGCCCATCCCGGGTGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGa < 2:333104/140‑1 (MQ=255) aTGTGCAGCCGCACCTATATGGTGAAAACGGCGATGAGCCTGACGCCGATCCCGGGTGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGaa < 1:744267/140‑1 (MQ=255) tgCAGCCGCACCTATATGGTGAAAACGGCGATGAGCCTGACGCCGATCCCGGGTGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACtg > 2:197260/1‑140 (MQ=255) tgCAGCCGCACCTATATGGTGAAAACGGCGATGAGCCTGACGCCGATCCCGGGTGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCAACCCCATTATCGTCATCTGCCGAGGTTTACAAGAACtg > 1:322712/1‑140 (MQ=255) cAGCCGCACCTATATGGTGAAAACGGCGATGAGCCTGACGCCGATCCCGGGTGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACtggt > 2:465630/1‑140 (MQ=255) aGCCGCACCTATATGGTGAAAACGGCGATGAGCCTGACGCCGATCCCGGGTGTGATCTTCTGAGCATGGCTATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACtggtg < 1:775237/140‑1 (MQ=255) tGGTGAAAACGGCGATGAGCCTGACGCCGATCCCGGGTGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCggtg < 2:581883/140‑1 (MQ=255) tGAAAACGGCGATGAGCCTGACGCCGATCCCGGGTGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGtt > 2:724671/1‑140 (MQ=255) tGAAAACGGCGATGAGCCTGACGCCGATCCCGGGTGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGAGGtt > 1:627424/1‑140 (MQ=255) aaaaCGGCGATGAGCCTGACGCCGATCCCGGGTGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTGGCCATCTGGCGGAGTTAACAAGAACAGGTGGTGGCAACCGGTGGTTCg > 2:227931/1‑140 (MQ=255) aaaaCGGCGATGAGCCTGACGCCGATCCCGGGTGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTATAAGCACTGGTGGTAGCAACCGGTGGTTCg > 1:271030/1‑140 (MQ=255) aaaaCGGCGATGAGCCTGACGCCGATCCCGGGTGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCgg < 1:774792/133‑1 (MQ=255) aaaaCGGCGATGAGCCTGACGCCGATCCCGGGTGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCgg > 2:774792/1‑133 (MQ=255) aaaaCGGCGATGAGCCTGACGCCGATCCCGGGTGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCgg > 2:96010/1‑133 (MQ=255) aaaaCGGCGATGAGCCTGACGCCGATCCCGGGTGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCgg < 1:96010/133‑1 (MQ=255) aaaaCGGCGATGAGCCTGACGCCGATCCCGGGTGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGTTCg > 2:611115/1‑140 (MQ=255) aaaaCGGCGATGAGCCTGACGCCGATCCCGGGTGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGTTCg > 2:787033/1‑140 (MQ=255) ggCGATGAGCCTGACGCCGATCCCGGGTGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGTTCGTTGCa > 2:38076/1‑140 (MQ=255) gCGATGAGCCTGACGCCGATCCCGGGTGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGTTCGTTGCAt < 1:643949/140‑1 (MQ=255) tGAGCCTGACGCCGACCCCGGGAGTTATCCTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGTTCGTTGCATCGCa < 1:227931/140‑1 (MQ=255) gAGCCTGACGCCGATCCCGGGTGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGTTCGTTGCATCGCaa < 2:122799/140‑1 (MQ=255) gCCGATCCCGGGTGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGTTCGTTGCATCGCAAGCTGTGCGa < 1:611115/140‑1 (MQ=255) gggTGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGTTCGTTGCATCGCAAGCTGTGCGAACAGCCTGa < 1:38076/140‑1 (MQ=255) | ACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGCCGCACCTATATGGTGAAAACGGCGATGAGCCTGACGCCGATCCCGGGCGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGTTCGTTGCATCGCAAGCTGTGCGAACAGCCTGA > NC_000913/1361257‑1361529 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |