Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,377,819	T→C	Y330H (TAC→CAC)	ycjS →	putative oxidoreductase YcjS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,377,819	0	T	C	100.0%	172.0 / NA	50	Y330H (TAC→CAC)	ycjS	putative oxidoreductase YcjS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/23);  total (27/23)

ATAACAACGGTGAATTAATGACGCTGATGCAACGGGAAATAGCAGACGACAACCGCCATTTGCGCAGCATGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGTACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAAACAGGGACGCGTGTGGAATTATGACCAGGCCAGTAACGTTATCAGAACCCCATTTCAGCCAGCATACCCTGAACAAGTATG  >  NC_000913/1377688‑1377944                                                                                                                                    |                                                                                                                              aTAACAACGGTGAATTAATGACGCTGATGCAACGGGAAATAGCAGACGACAACCGCCATTTGCGCAGCATGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACatcatc                                                                                                                       >  1:800653/1‑140 (MQ=255) aTAACAACGGTGAATTAATGACGCTGATGCAACGGGAAATAGCAGACGACAACCGCCATTTGCGCAGCATGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACatcatc                                                                                                                       >  2:154892/1‑140 (MQ=255) aTAACAACGGTGAATTAATGACGCTGATGCAACGGGAAATAGCAGACGACAACCGCCATTTGCGCAGCATGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACatcatc                                                                                                                       >  2:561217/1‑140 (MQ=255) aTAACAACGGTGAATTAATGACGCTGATGCAACGGGAAATAGCAGACGACAACCGCCATTTGCGCAGCATGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACatcatc                                                                                                                       >  2:23973/1‑140 (MQ=255) aTAACAACGGTGAATTAATGACGCTGATGCAACGGGAAATAGCAGACGACAACCGCCATTTGCGCAGCATGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACatcatc                                                                                                                       >  2:532910/1‑140 (MQ=255) aTAACAACGGTGAATTAATGACGCTGATGCAACGGGAAATAGCAGACGACAACCGCCATTTGCGCAGCATGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACatcatc                                                                                                                       >  2:28292/1‑140 (MQ=255) aTAACAACGGTGAATTAATGACGCTGATGCAACGGGAAATAGCAGACGACAACCGCCATTTGCGCAGCATGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGATAACCGACGCCGAGCAGGGGCACatcatc                                                                                                                       >  1:89770/1‑140 (MQ=255)           tGAATTAATGACGCTGATGCAACGGGAAATAGCAGACGACAACCGCCATTTGCGCAGCATGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGATAGCCGACGTCGAGCAAGGGCACCTCATCCAGCAACtgg                                                                                                             >  1:278871/1‑140 (MQ=255)           tGAATTAATGACGCTGATGCAACGGGAAATAGCAGACGACAACCGCCATTTGCGCAGCATGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACtgg                                                                                                             >  2:445716/1‑140 (MQ=255)            gAATTAATGACGCTGATGCAACGGGAAATAGCAGACGACAACCGCCATTTGCGCAGCATGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGATAACCGACGCCGAGTAGCGGCACAGCATCCAGCAACtggt                                                                                                            >  1:339278/1‑140 (MQ=255)               ttAATGACGCTGATGCAACGGGTAAAAGCCGACGACACCCGCCATTTGCGCAGCATGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTggc                                                                                                         <  1:232588/140‑1 (MQ=255)               ttAAGGACGCTGATGCAACGGATAATAGCAGTCGACAACCGCCATTTGAGCAGCATGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTggc                                                                                                         <  2:335115/140‑1 (MQ=255)                       gCTGATGCAACGGGAAATAGCAGACGACAACCGCCATTTGCGCAGCATGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGt                                                                                                 >  1:5698/1‑140 (MQ=255)                       gCTGATGCAACGGGAAATAGCAGACGACAACCGCCATTTGCGCAGCATGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACATAATCCAGCAAATGGTGGCGGCGTTGt                                                                                                 >  1:242443/1‑140 (MQ=255)                         tGATGCAACGGGAAATAGCAGACGACAACCGCCATTTGCGCAGCATGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTAt                                                                                               >  1:174467/1‑140 (MQ=255)                            tGCAACGGGAAATAGCAGACGACAACCGCCATTTGCGCAGCATGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCaa                                                                                            >  2:665621/1‑140 (MQ=255)                                 cGGGAAATAGCAGACGACAACCGCCATTTGCGCAGCATGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGc                                                                                       >  2:708333/1‑140 (MQ=255)                                     aaaTAGCAGACGACAACCGCCATTTGCGCAGCATGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGaa                                                                                   >  1:110706/1‑140 (MQ=255)                                           cAGACGACAACCGCCATTTGCGCAGCATGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGGTAGCCGACGCCGAGCAGGGGCATATCATCCATCAAGTGGTGGCGGCGTAGTATTGATCCGCAGAAACAggg                                                                             >  2:361959/1‑140 (MQ=255)                                               cgacAACCGCCATTTGCGCAGCCTGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGGTAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAAACAGGGAcgc                                                                         <  1:361959/140‑1 (MQ=255)                                               cgacAACCGCCATTTGCGCAGCATGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAAACAGGGAcgc                                                                         >  2:208298/1‑140 (MQ=255)                                                    aCCGCCATTTGCGCAGCATGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAAACAGGGACGCGtgtg                                                                    <  2:117852/140‑1 (MQ=255)                                                     ccGCCATTTGCGCAGCATGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAAACAGGGACGCGTGTgg                                                                   >  2:470284/1‑140 (MQ=255)                                                         cATTTGCGCAGCATGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAAACAGGGACGCGTGTGGAAtt                                                               <  1:790811/140‑1 (MQ=255)                                                          aTTTGCGCAGCATGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGAATCCATCCGCAGAAACAGGGACGCGTGTGGAATTa                                                              >  2:303894/1‑140 (MQ=255)                                                              gcgcAGCATGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAAACAGGGACGCGTGTGGAATTATGAc                                                          <  1:154892/140‑1 (MQ=255)                                                              gcgcAGCATGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAAACAGGGACGCGTGTGGAATTATGAc                                                          <  2:5698/140‑1 (MQ=255)                                                              gcgcAGCATGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAAACAGGGACGCGTGTGGAATTATGAc                                                          <  1:28292/140‑1 (MQ=255)                                                              gcgcAGCATGGAAGCCTATATCCATCACGTATAGGCCAAACCCGTGATTATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAAACAGGGACGCGTGTGGAATTATGAc                                                          <  2:242443/140‑1 (MQ=255)                                                               cgcAGCATGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGTAGAAACAGGGACGCGTGTGGAATTATGAcc                                                         <  2:174467/140‑1 (MQ=255)                                                                 cagcaTGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAAACAGGGACGCGTGTGGAATTATGACCAg                                                       <  1:532910/140‑1 (MQ=255)                                                                   gcaTGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGATAACCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAAACAGGGACGCGTGTGGAATTATGACCAGGc                                                     <  2:89770/140‑1 (MQ=255)                                                                           gCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAAACAGGGACGCGTGTGGAATTATGACCAGGCCAGTAACg                                             <  1:106165/140‑1 (MQ=255)                                                                                     atcaCGTACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAAACAGGGACGCGTGTGGAATTATGACCAGGCCAGTAACGTTATCAGAAc                                   >  2:735316/1‑140 (MQ=255)                                                                                       caCGTACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAAACAGGGACGCGTGTGGAATTATGACCAGGCCAGTAACGTTATCAGAAccc                                 >  2:487741/1‑140 (MQ=255)                                                                                         cGTACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAAACAGGGACGCGTGTGGAATTATGACCAGGCCAGTAACGTTATCAGAACCCCa                               >  2:200605/1‑140 (MQ=255)                                                                                           tACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAAACAGGGACGCGTGTGGAATTATGACCAGGCCAGTAACGTTATCAGAACCCCAtt                             <  1:23973/140‑1 (MQ=255)                                                                                           tACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAAACAGGGACGCGTGTGGAATTATGACCAGGCCAGTAACGTTATCAGAACCCCAtt                             <  1:561217/140‑1 (MQ=255)                                                                                             cAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAAACAGGGACGCGTGTGGAATTATGACCAGGCCAGTAACGTTATCAGAACCCCATTTc                           <  1:665621/140‑1 (MQ=255)                                                                                              aGGGCAAGCCCGTGATGATAGCTGACGCCGACCAGTGGCGCATCATCCTGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAAACA‑GGACGCGTGTGGAATTATGACCAGGCCAGTAACGTTATCAGAACCCCATTTcag                         <  2:278871/140‑1 (MQ=255)                                                                                               gggCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAAACAGGGACGCGTGTGGAATTATGACCAGGCCAGTAACGTTATCAGAACCCCATTTcag                         <  2:800653/140‑1 (MQ=255)                                                                                               gggCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAAACAGGGACGCGTGTGGAATTATGACCAGACCAGTAACGTTATTAGAACCCCATTTcag                         >  1:221266/1‑140 (MQ=255)                                                                                                ggCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAAACAGGGACGCGTGTGGAATTATGACCAGGCCAGTAACGTTATCAGAACCCCATTTcagc                        >  1:553526/1‑140 (MQ=255)                                                                                                  cAAGCCCGTGATGATAGCCGACGCTGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAAACAGGGACGCGTGTGGAATTATGACTAGGCCAGTAACGTTATCCGAACCCCATTTcagcca                      >  2:327085/1‑140 (MQ=255)                                                                                                     gCCCGTGATGATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGCTGTATCAATCCGCAGAAACAGGGACGCGTGTGGAATTATGACCAGGCCAGTAACGTTATCAGAACCCCATTTCAGCCAGCa                   <  2:570961/140‑1 (MQ=255)                                                                                                       ccGTGATGATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAAACAGGGACGCGTGTGGAATTATGACCAGGCCAGTAACGTTATCAGAACCCCATTTCAGCCAGCATa                 <  1:733126/140‑1 (MQ=255)                                                                                                         gTGATGATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAAACAGGGACGCGTGTGGAATTATGACCAGGCCAGTAACGTTATCAGAACCCCATTTCAGCCAGCATAcc               <  1:735316/140‑1 (MQ=255)                                                                                                         gTGATGATAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAAACAGGGACGCGTGTGGAATTATGACCAGGCCAGTAACGTTATCAGAACCCCATTTCAGCCAGCATAcc               <  1:725003/140‑1 (MQ=255)                                                                                                          tgatgaTAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAAACAGGGACGCGTGTGGAATTATGACCAGGCCAGTAACGTTATCAGAACCCCATTTCAGCCAGCATAccc              >  2:45934/1‑140 (MQ=255)                                                                                                          tgatgaTAGCCGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAAACAGGGACGCGTGTGGAATTATGACCAGGCCAGTAACGTTATCAGAACCCCATTTCAGCCAGCATAccc              >  1:796405/1‑140 (MQ=255)                                                                                                                   ccGACGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAAACAGGGACGCGTGTGGAATTATGACCAGGCCAGTAACGTTATCAGAACCCCATTTCAGCCAGCATACCCTGAACAAGt     <  1:708333/140‑1 (MQ=255)                                                                                                                      aCGCCGAGCAGGGGCACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAAACAGGGACGCGTGTGGAATTATGACCAGGCCAATAACGTTAACAGGACCCCATTTCAGCCAGCATACCCTGAACAAGTATg  >  2:253436/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                              ATAACAACGGTGAATTAATGACGCTGATGCAACGGGAAATAGCAGACGACAACCGCCATTTGCGCAGCATGGAAGCCTTTATCAATCACGTACAGGGCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGTACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAAACAGGGACGCGTGTGGAATTATGACCAGGCCAGTAACGTTATCAGAACCCCATTTCAGCCAGCATACCCTGAACAAGTATG  >  NC_000913/1377688‑1377944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑