Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,438,306	A→G	Y827H (TAT→CAT)	ydbK ←	putative pyruvate‑flavodoxin oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,438,306	0	A	G	100.0%	149.8 / NA	42	Y827H (TAT→CAT)	ydbK	putative pyruvate‑flavodoxin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/18);  total (24/18)

TGCCGGCCCACGACCGTTGGCATCGGTGGTATACGGTGTAGAGGGCAGGTTACCGCCATAAATTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATT  >  NC_000913/1438175‑1438442                                                                                                                                    |                                                                                                                                         tGCCGGCCCACGACCGTTGGCATCGGTGGTATGCGGTGTAGAGGGCAGGTTACCGCCATAAATTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGtct                                                                                                                                  >  1:653999/1‑140 (MQ=255)          ccgcccGTTGGCATGGGGGGTATGGGGTGAAGGGGCCGGTTTCCCGCCGTAAATTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAAc                                                                                                                         <  2:364073/136‑1 (MQ=255)            gccggtgggCATGGGTGGTATGGGGTGTAGGGGGCAGGTTACCGCCATAAATTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCg                                                                                                                       <  2:687614/133‑1 (MQ=255)                   ggcttgggtggtAGGGGGTGTGGGGGGCGGGTTCCCGCCATAAATTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGagcaag                                                                                                                <  2:716977/134‑1 (MQ=255)                         ggtggtAGGGGGTGTAGGGGGAAGTTTACCGCCATAAATTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTg                                                                                                          <  2:574624/140‑1 (MQ=255)                                    gtgtAGAGGGCAGGTTACCGCCATAAATTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAAc                                                                                               >  1:673569/1‑140 (MQ=255)                                     tgtAGAGGGCAGGTTACCGCCATAAATTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACa                                                                                              >  2:429337/1‑140 (MQ=255)                                     tgtAGAGGGCAGGTTACCGCCATAAATTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACa                                                                                              >  1:597891/1‑140 (MQ=255)                                       tAGAGGGCAGGTTACCGCCATAAATTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGc                                                                                            >  1:685542/1‑140 (MQ=255)                                        agagGGCGGGTCGCCGCCATAAATTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCg                                                                                           <  1:486630/140‑1 (MQ=255)                                               aGGTTACCGCCATAAATTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCTGAGTAAGCACCTGAATATTCAAACAGCGGTGTAAt                                                                                    >  2:345891/1‑140 (MQ=255)                                                  ttACCGCCATAAATTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAg                                                                                 <  2:688042/140‑1 (MQ=255)                                                      cGCCATAAATTGAAGAACAGCCAGTGGCGTTAGTGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGc                                                                             >  2:112090/1‑140 (MQ=255)                                                           tAAATTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTg                                                                              >  1:125457/1‑134 (MQ=255)                                                           tAAATTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTg                                                                              <  2:125457/134‑1 (MQ=255)                                                           tAAATTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGCGATGt                                                                        >  2:767217/1‑140 (MQ=255)                                                           tAAATTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGCGATGt                                                                        >  1:786890/1‑140 (MQ=255)                                                            aaaTTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGCGATGTa                                                                       <  2:597891/140‑1 (MQ=255)                                                                tGAAGAACAGCCAGTGTGGTTAGCGATCAACGTGCTGTCCCCATAGAGATGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGCGATGTACGaa                                                                   <  2:371804/140‑1 (MQ=255)                                                                    gaaCAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGCGATGTACGAATATc                                                               >  1:736199/1‑140 (MQ=255)                                                                    gaaCAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGCGATGTACGAATATc                                                               >  1:205982/1‑140 (MQ=255)                                                                     aaCAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGCGATGTACGAATATCa                                                              <  2:653999/140‑1 (MQ=255)                                                                     aaCAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGCGATGTACGAATATCa                                                              <  2:685542/140‑1 (MQ=255)                                                                       cAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGCGATGTACGAATATCAAt                                                            >  2:21188/1‑140 (MQ=255)                                                                          ccAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGCGATGTACGAATATCAATACg                                                         >  2:198704/1‑140 (MQ=255)                                                                            aGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGCGATGTACGAATATCAATACGtt                                                       >  1:739116/1‑140 (MQ=255)                                                                                  gTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGtt                                                 >  2:735762/1‑140 (MQ=255)                                                                                           cAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGt                                        <  1:198704/140‑1 (MQ=255)                                                                                            aaCATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTc                                       >  1:438327/1‑140 (MQ=255)                                                                                                 ccGGTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGAttt                                  >  2:715991/1‑140 (MQ=255)                                                                                                  cGGTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTc                                 >  2:409576/1‑140 (MQ=255)                                                                                                    gTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTg                               >  2:522211/1‑140 (MQ=255)                                                                                                    gTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTg                               >  1:779761/1‑140 (MQ=255)                                                                                                    gTCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCGGCGATGTAGGAAAATCTATACGTTCGAGTTAGCTACGGTCGATTTCTg                               >  2:213585/1‑140 (MQ=255)                                                                                                     tCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTgg                              <  2:438327/140‑1 (MQ=255)                                                                                                     tCGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTgg                              <  2:673569/140‑1 (MQ=255)                                                                                                      cGCCATAGAGCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGc                             <  1:21188/140‑1 (MQ=255)                                                                                                            agagCTGAGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTg                       <  1:409576/140‑1 (MQ=255)                                                                                                                   aGTCAGTAATTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGgaaga                >  1:800622/1‑140 (MQ=255)                                                                                                                           aTTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTaa        <  2:205982/140‑1 (MQ=255)                                                                                                                           aTTTAATATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTaa        <  2:736199/140‑1 (MQ=255)                                                                                                                               aaTATGCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGa    >  1:788771/1‑140 (MQ=255)                                                                                                                                 tatgCGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGAtt  <  1:767217/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                         TGCCGGCCCACGACCGTTGGCATCGGTGGTATACGGTGTAGAGGGCAGGTTACCGCCATAAATTGAAGAACAGCCAGTGGCGTTAGCGATCAACATCCGGTCGCCATAGAGCTGAGTCAGTAATTTAATATACGGCGTCTCGCCACAACCGGAGCAAGCACCTGAATATTCAAACAGCGGTGTAATCAGCTGCGATGTACGAATATCAATACGTTCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATT  >  NC_000913/1438175‑1438442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑