Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,455,822	G→A	G220D (GGT→GAT)	paaC →	phenylacetyl‑CoA 1,2‑epoxidase, structural subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,455,822	0	G	A	100.0%	146.9 / NA	45	G220D (GGT→GAT)	paaC	phenylacetyl‑CoA 1,2‑epoxidase, structural subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/25);  total (20/25)

ATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCG  >  NC_000913/1455690‑1455951                                                                                                                                     |                                                                                                                                  atatTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGATaaaaaa                                                                                                                            <  2:811105/140‑1 (MQ=255)  tatTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGATAAAAAAg                                                                                                                           >  1:814990/1‑140 (MQ=255)      gCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGATAAAAAAGGACt                                                                                                                       >  2:622013/1‑140 (MQ=255)       cACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGATAAAAAAGGACTg                                                                                                                      <  1:406479/140‑1 (MQ=255)           gAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGATAAAAAAGGACTGCATa                                                                                                                  >  2:740166/1‑140 (MQ=255)            aGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGATAAAAAAGGACTGCATAc                                                                                                                 >  1:707411/1‑140 (MQ=255)            aGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGATAAAAAAGGACTGCATAc                                                                                                                 >  1:70081/1‑140 (MQ=255)                aaGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGATAAAAAAGGACTGCATACCGaa                                                                                                             <  2:201042/140‑1 (MQ=255)                aaGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGATAAAAAAGGACTGCATACCGaa                                                                                                             <  1:3482/140‑1 (MQ=255)                aaGAGGGTATTGCGGCTGATCCCCGCACTATACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGATAAAAAAGGACTGCATACCGaa                                                                                                             <  1:224542/140‑1 (MQ=255)                 agagGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGATAAAAAAGGACTGCATACCGAac                                                                                                            <  1:740166/140‑1 (MQ=255)                      gTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGATAAAAAAGGACTGCATACCGAACACCtt                                                                                                       >  1:735836/1‑140 (MQ=255)                           gCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGAT‑AAAAAGGACTGCATACCGAACACCTTGGAccc                                                                                                 <  1:162019/140‑1 (MQ=255)                              gTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGATAAAAAAGGACTGCATACCGAACACCTTGGACCCAt                                                                                               >  2:709132/1‑140 (MQ=255)                               ttGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGATAAAAAAGGACTGCATACCGaa                                                                                                             <  1:735923/125‑1 (MQ=255)                               ttGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGATAAAAAAGGACTGCATACCGaa                                                                                                             >  2:735923/1‑125 (MQ=255)                               ttGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTAGCGCACTGGCGATGAAAAAGGACTGCTTACCGAACACCTTGGACCCATg                                                                                              >  2:285332/1‑140 (MQ=255)                                 gATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGATAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCt                                                                                            <  2:735836/140‑1 (MQ=255)                                             ttACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGATAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCa                                                                                >  2:130092/1‑140 (MQ=255)                                              tACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGATAAAAAAGGACTGCGTACCGAATACCGTGTACCCATGCTGGCAGAAATGCAg                                                                               >  1:387433/1‑140 (MQ=255)                                              tACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGATAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAg                                                                               <  1:622013/140‑1 (MQ=255)                                               aCGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGATAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGt                                                                              <  2:427057/140‑1 (MQ=255)                                                         tGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGATAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCg                                                                    >  2:818420/1‑140 (MQ=255)                                                                      tttttGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGATAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGt                                                       <  1:818420/140‑1 (MQ=255)                                                                        tttGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGATAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCa                                                     <  1:192490/140‑1 (MQ=255)                                                                          tGCCGGGATCAACGAAGTCACATTGAACGTTTCGCTAGACCAGGCGTAACGCACTGGCGATAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGc                                                   <  2:387433/140‑1 (MQ=255)                                                                             cGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGATAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAAt                                                <  1:145428/140‑1 (MQ=255)                                                                             cGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGATAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAAt                                                <  2:707411/140‑1 (MQ=255)                                                                                    aaCGAAGCCACATTGCACGTACCGCCAGATCAGGCGTATCGCACTGGCGATAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACa                                         <  1:285332/140‑1 (MQ=255)                                                                                    aaCGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGATAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACa                                         <  2:814990/140‑1 (MQ=255)                                                                                    aaCGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGATAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACa                                         <  2:70081/140‑1 (MQ=255)                                                                                          gCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGATAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCAAGCGTGTCTTGCCCGGTCAGCAATGGTAACAgaggag                                   >  1:352474/1‑140 (MQ=255)                                                                                           ccACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGATAAAAAAGGACTGTATACCGAACACCTTGGACCCATGCCGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGgaga                                  <  2:608818/140‑1 (MQ=255)                                                                                           ccACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGATAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGgaga                                  <  1:74040/140‑1 (MQ=255)                                                                                                 tGAACGTACCGCAAGAGCAGGCCTACCGCACTGGCGAAAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTa                            <  1:392707/140‑1 (MQ=255)                                                                                                     cGTACCGCAAGAGCAGGCGTATCGCACTGGCGATAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCa                        >  2:153727/1‑140 (MQ=255)                                                                                                        aCCGCAAGAGCAGGCGTATCGCACTGGCGATAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACg                     >  2:356479/1‑140 (MQ=255)                                                                                                         ccGCAAGAGCAGGCGTATCGCATTGGCGATACAAACGGAGTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGt                    <  2:352474/140‑1 (MQ=255)                                                                                                         ccGCAAGAGCAGGCGTATCGCACTGGCGATAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGt                    >  1:412263/1‑140 (MQ=255)                                                                                                             aaGAGCAGGCGTATCGCACTGGCGATAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTGTTGCCCGGCCAGCAATGGTAACAGAGGAGACGGGTATGCAACGTCTgg                >  2:313837/1‑140 (MQ=255)                                                                                                              agagCAGGCGTATCGCACTGGCGATAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGc               <  1:356479/140‑1 (MQ=255)                                                                                                                    ggCGTATCGCACTGGCGATAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCGACGTCTGGCCACCAt         >  1:212101/1‑140 (MQ=255)                                                                                                                           cGCACTGGCGATAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGCGTAAGCAACGTCTAGCCACCATTGCACCg  >  1:392787/1‑140 (MQ=255)                                                                                                                               cTGGCGATAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGTGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGc               >  1:92136/1‑123 (MQ=255)                                                                                                                               cTGGCGATAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGTGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGc               <  2:92136/123‑1 (MQ=255)                                                                                                                                     |                                                                                                                                  ATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCG  >  NC_000913/1455690‑1455951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑