Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,459,273	A→G	I74V (ATC→GTC)	paaH →	3‑hydroxyadipyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,459,273	0	A	G	100.0%	83.3 / NA	25	I74V (ATC→GTC)	paaH	3‑hydroxyadipyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/11);  total (14/11)

CAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCCGCGCAATCGACGGGATACACGCGCGGCTAAATTCACGCGTGACGCGGGGAAAACTGACTGCTGAAACCTGTGAACGCACATTGAAACGCCTGATCCCGGTGACCGATATTCACGCGCTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAGCGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAA  >  NC_000913/1459141‑1459409                                                                                                                                     |                                                                                                                                         cAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCCGCGCAATCGACGGGATACACGCGCGGCTAAATTCACGCGTGACGCGGGGAAAACTGACTGCTGAAACCTGTGAACGCACATTGAAACGCCTGGTCCCGGt                                                                                                                                   <  2:520854/140‑1 (MQ=255)    gTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCCGCGCAATCGACGGGATACACGCGCGGCTAAATTCACGCGTGACGCGGGGAAAACTGACTGCTGAAACCTGTGAACGCACATTGAAACGCCTGGTCCCGGTGAc                                                                                                                                <  2:791796/140‑1 (MQ=255)    gTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCCGCGCAATCGACGGGATACACGCGCGGCTAAATTCACGCGTGACGCGGGGAAAACTGACTGCTGAAACCTGTGAACGCACATTGAAACGCCTGGTCCCGGTGAc                                                                                                                                <  2:423100/140‑1 (MQ=255)         aCTGTATGACATTTCTGCTGAAGCGCTGACCCGCGCAATCGACGGGACACACGCGCGGCTAAATTCACGCGTGACGCGGGGAAAACTGACTGCTGAAACCTGTGAACGCACATTGAAACGCCTGGTCCCGCGGACCGata                                                                                                                           >  1:804584/1‑140 (MQ=255)              aTGACATTTCTGCTGAAGCGCTGACCCGCGCAATCGACGGGATACACGCGCGGCTAAATTCACGCGTGACGCGGGGAAAACTGACTGCTGAAACCTGTGAACGCACATTGAAACGCCTGGTCCCGGTGACCGATATTCAc                                                                                                                      >  1:650912/1‑140 (MQ=255)               tGACATTTCTGCTGAAGCGCTGACCCGCGCAATCGACGGGATACACGCGCGGCTAAATTCACGCGTGACGCGGGGAAAACTGACTGCTGAAACCTGTGAACGTACATTGAAACGACTTGTCCCGGTGACCGATATTCAcg                                                                                                                     >  1:394401/1‑140 (MQ=255)                     ttCTGCTGAAGCGCTGACCCGCGCAATCGACGGGATACACGCGCGGCTAAATTCACGCGTGACGCGGGGAAAACTGACTGCTGAAACCTGTGAACGCACATTGAAACGCCTGGTCCCGGTGACCGATATTCACGCGCTgg                                                                                                               >  1:654073/1‑140 (MQ=255)                     ttCTGCTGAAGCGCTGACCCGCGCAATCGACGGGATACACGCGCGGCTAAATTCACGCGTGACGCGGGGAAAACTGACTGCTGAAACCTGTGAACGCACATTGAAACGCCAGGTGCCAGTGACCTATATTCACGCGCTgg                                                                                                               >  1:390793/1‑140 (MQ=255)                      tCTGCTGAAGCGCTGACCCGCGCAATCGACGGGATACACGCGCGGCTAAATTCACGCGTGACGCGGGGAAAACTGACTGCTGAAACCTGTGAACGCACATTGAAACGCCTGGTCCCGGTGACCGATATTCACGCGCTGGc                                                                                                              <  2:773440/140‑1 (MQ=255)                       ctgctgAAGCGCTGACCCGCGCAATCGACGGGATACACGCGCGGCTAAATTCACGCGTGACGCGGGGAAAACTGACTGCTGAAACCTGTGAACGCACATTGAAACGCCTGGTCCCGGTGACCGATATTCACGCGCTGGCa                                                                                                             >  1:47866/1‑140 (MQ=255)                          ctgAAGCGGTGACCCGGCCAATTGACGGGATTCACCCGCGGCTAAATTTACGCGTGACGCGGGGAAAACTGACTGCTGAAACCTGTGAACGCACATTGAAACGCCTGGTCCCGGTGACCGATATTCACGCGCTGGCAGCt                                                                                                          <  2:394401/140‑1 (MQ=255)                                cgcTGACCCGCGCAATCGACGGGATACACGCGCGGCTAAATTCACGCGTGACGCGGGGAAAACTGACTGCTGAAACCTGTGAACGCACATTGAAACGCCTGGTCCCGGTGACCGATATTCACGCGCTGGCAGCTGCGGAc                                                                                                    <  2:47866/140‑1 (MQ=255)                                                       aTACACGCGCGGCTAAATTCACGCGTGACGCGGGGAAAACTGACTGCTGAAACCTGTGAACGCACATTGAAACGCCTGGTCCCGGTGACCGATATTCACGCGCTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGa                                                                             <  2:650912/140‑1 (MQ=255)                                                         acacGCGCGGCTAAATTCACGCGTGACGCGGGGAAAACTGACTGCTGAAACCTGTGAACGCACATTGAAACGCCTGGTCCCGGTGACCGATATTCACGCGCTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAAc                                                                           >  1:460866/1‑140 (MQ=255)                                                                cgGCTAAATTCACGCGTGACGCGGGGAAAACTGACTGCTGAAACCTGTGAACGCACATTGAAACGCCTGGTCCCGGTGACCGATATTCACGCGCTGGCAGATGCTGACTTGGTCATTGAAGCGGCGTCTGAACGTCTGGa                                                                    >  2:304366/1‑140 (MQ=255)                                                                           aCGCGTGACGCGGGGAAAACTGACTGCTGAAACCTGTGAACGCACATTGAAACGCCTGGTCCCGGTGACCGATATTCACGCGCTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAg                                                         >  1:655940/1‑140 (MQ=255)                                                                            cgcgTGACGCGGGGAAAACTGACTGCTGAAACCTGTGAACGCACATTGAAACGCCTGGTCCCGGTGACCGATATTCACGCGCTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAgc                                                        <  2:655940/140‑1 (MQ=255)                                                                             gcgTGACGGGGGGAAACATGACTGCTGAAAGATTTGAACACACATTGACACGCCTGGTCCCGGTGACCGATATTCACGCGCTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAgcg                                                       <  2:390793/140‑1 (MQ=255)                                                                                         gAAAACTGACTGCTGAAACCTGTGAACGCACATTGTAACGCCTGGTCCCGGTGACCGATATTCACGCGCTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAGCGCTCTTTGCACAg                                           <  2:104801/140‑1 (MQ=255)                                                                                          aaaaCTGACTGCTGAGACCTGTAAACGCACATTGAAAAGCCTGGTCCCGGTGACCGATATTCACGCGCTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAGCGCTCTTTGCACAGc                                          <  1:304366/140‑1 (MQ=255)                                                                                                 actgCTGAAACCTGTGAACGCACATTGAAACGCCTGGTCCCGGTGACCGATATTCACGCGCTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAGCGCTCTTTGCACAGCTGGCGGa                                   >  2:439285/1‑140 (MQ=255)                                                                                                               tgAACGCACATTGAAACGCCTGGTCCCGGTGACCGATATTCACGCGCTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAGGCGCTCTGTGCACATCTGGCGAAAGTTTGCCCGCcac                     >  1:243682/1‑140 (MQ=255)                                                                                                               tgAACGCACATTGAAACGCCTGGTCCCGGTGACCGATATTCACGCGCTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAGCGCTCTATGCACAACTGGCGGAAGTTTGCCCGCcac                     >  1:261869/1‑140 (MQ=255)                                                                                                                           gAAACGCCTGGTCCCGGTGACCGATATTCACGCGCTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAGCGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGa         >  1:452830/1‑140 (MQ=255)                                                                                                                                  cTGGTCCCGGTGACCGATATTCACGCGCTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAGCGCTCTTTGCACAGCTGGCGGAAGTGTGCTCGCCACAAACGCTATTGACCACTaa  >  1:289808/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                         CAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCCGCGCAATCGACGGGATACACGCGCGGCTAAATTCACGCGTGACGCGGGGAAAACTGACTGCTGAAACCTGTGAACGCACATTGAAACGCCTGATCCCGGTGACCGATATTCACGCGCTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAGCGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAA  >  NC_000913/1459141‑1459409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑