Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,462,242	T→C	Y40H (TAT→CAT)	paaK →	phenylacetate‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,462,242	0	T	C	100.0%	299.8 / NA	87	Y40H (TAT→CAT)	paaK	phenylacetate‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (46/41);  total (46/41)

CAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATG  >  NC_000913/1462105‑1462376                                                                                                                                          |                                                                                                                                       cAACCTGCTAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCAt                                                                                                                                      >  1:645666/1‑140 (MQ=255)       gCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGgcgc                                                                                                                                <  1:125189/140‑1 (MQ=255)              cccTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGCAAAAGTTCCGATGCATCGGCGCAAATTCg                                                                                                                         >  1:314933/1‑140 (MQ=255)               ccTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGa                                                                                                                        >  1:486072/1‑140 (MQ=255)                    aaTGATAACCAATACTAAGCTTTACCCGAGTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACgcag                                                                                                                   <  1:284227/140‑1 (MQ=255)                    aaTGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACgcag                                                                                                                   <  2:497674/140‑1 (MQ=255)                      tGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACgcagca                                                                                                                 <  2:607771/140‑1 (MQ=255)                        aTAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAgg                                                                                                               >  2:493588/1‑140 (MQ=255)                        aTAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAgg                                                                                                               >  2:447780/1‑140 (MQ=255)                        aTAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAgg                                                                                                               >  1:596858/1‑140 (MQ=255)                        aTAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAgg                                                                                                               >  1:568569/1‑140 (MQ=255)                        aTAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCAATGCATAGGCGCATATTCGACGCAGCAgg                                                                                                               >  1:246927/1‑140 (MQ=255)                         tAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTTAAACACGCGTATGAAATTGTGCCGATGCTTCGGCGCAATTTCGACGCAGCAGGc                                                                                                              >  1:321007/1‑140 (MQ=255)                         tAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAGTGTGCCGTTGCTTCGGCGCAAGTTCGACGCAGCAGGc                                                                                                              >  1:225945/1‑140 (MQ=255)                         tAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGc                                                                                                              >  2:818338/1‑140 (MQ=255)                         tAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGc                                                                                                              >  1:801221/1‑140 (MQ=255)                         tAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCAGCGCAAATTCGACGCAGCAGGc                                                                                                              >  1:290403/1‑140 (MQ=255)                           aCCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGt                                                                                                            >  1:686073/1‑140 (MQ=255)                            ccAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTa                                                                                                           <  2:486072/140‑1 (MQ=255)                              aaTACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACa                                                                                                         >  1:648734/1‑140 (MQ=255)                                tACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAA‑TCGACGCAGCAGGCGTACATcc                                                                                                      >  2:423816/1‑140 (MQ=255)                                       cTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCtgatga                                                                                                >  1:60237/1‑140 (MQ=255)                                       cTTGACCCGATTGAAACCGCGTC‑GTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTgatgat                                                                                               >  2:661711/1‑140 (MQ=255)                                          gACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGAttt                                                                                             >  1:474799/1‑140 (MQ=255)                                          gACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGAttt                                                                                             >  1:363991/1‑140 (MQ=255)                                           aCCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCAACGGAGGAGGCGTTCATCCTGATGATTTc                                                                                            >  1:234426/1‑140 (MQ=255)                                                aTTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGa                                                                                       >  2:569247/1‑140 (MQ=255)                                                 ttGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGaa                                                                                      >  1:757782/1‑140 (MQ=255)                                                 ttGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGaa                                                                                      >  1:715185/1‑140 (MQ=255)                                                          cgTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGAc                                                                             >  1:460000/1‑140 (MQ=255)                                                          cgTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGAc                                                                             <  2:645666/140‑1 (MQ=255)                                                          cgTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGAc                                                                             >  1:652925/1‑140 (MQ=255)                                                          cgTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGAc                                                                             <  2:596858/140‑1 (MQ=255)                                                                   atgaGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTaaa                                                                    <  2:648734/140‑1 (MQ=255)                                                                   atgaGTTACAGGCGTTGAAAACTCAGCGTCTGAAACGGACGCTCAAACCCGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTaaa                                                                    <  2:314933/140‑1 (MQ=255)                                                                        ttACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTcc                                                               <  1:816097/140‑1 (MQ=255)                                                                        ttACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTcc                                                               >  1:464161/1‑140 (MQ=255)                                                                           cAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCAGCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGGCCTGCGTAAATTTCCCTg                                                            <  1:178439/140‑1 (MQ=255)                                                                             ggCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAAttt                                                                 <  2:42203/133‑1 (MQ=255)                                                                             ggCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAAttt                                                                 >  1:42203/1‑133 (MQ=255)                                                                             ggCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTa                                                          <  1:661711/140‑1 (MQ=255)                                                                                 ttGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTaccac                                                      <  1:447780/140‑1 (MQ=255)                                                                                 ttGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTaccac                                                      <  2:363991/140‑1 (MQ=255)                                                                                 ttGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTaccac                                                      <  2:757782/140‑1 (MQ=255)                                                                                 ttGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTaccac                                                      <  1:493588/140‑1 (MQ=255)                                                                                 ttGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTaccac                                                      <  2:60237/140‑1 (MQ=255)                                                                                 ttGCAAACACAGCGTATGAAATTGAGGCTTAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTaccac                                                      <  1:358624/140‑1 (MQ=255)                                                                                     aaaCACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCaaa                                                  <  2:464161/140‑1 (MQ=255)                                                                                         acaGCGTCTGAAATGGAAGCTCAAACACGCGCATGGAAAAGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAgg                                              <  2:290403/140‑1 (MQ=255)                                                                                            gCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATc                                           <  1:818338/140‑1 (MQ=255)                                                                                            gCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATc                                           >  1:533188/1‑140 (MQ=255)                                                                                             cGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTg                                                            >  1:187436/1‑122 (MQ=255)                                                                                             cGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTg                                                            <  2:187436/122‑1 (MQ=255)                                                                                             cGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCt                                          >  2:452287/1‑140 (MQ=255)                                                                                             cGTCTGAAATGGACGCTCAAACACGCGTATAAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCt                                          >  1:708380/1‑140 (MQ=255)                                                                                              gTCTGAAATGGACGCTCAAACACGCGTATGATAGTGTGCTGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTg                                         <  2:371893/140‑1 (MQ=255)                                                                                              gTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTg                                         >  1:124089/1‑140 (MQ=255)                                                                                              gTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTg                                         <  1:569247/140‑1 (MQ=255)                                                                                              gTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTg                                         >  1:601602/1‑140 (MQ=255)                                                                                                 tGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTgcgc                                      >  2:793052/1‑140 (MQ=255)                                                                                                 tGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTgcgc                                      <  2:652925/140‑1 (MQ=255)                                                                                                 tGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTgcgc                                      <  2:474799/140‑1 (MQ=255)                                                                                                   aaaTGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGa                                    >  1:460280/1‑140 (MQ=255)                                                                                                    aaTGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGAc                                   <  1:541595/140‑1 (MQ=255)                                                                                                    aaTGGACGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGAc                                   <  2:171713/140‑1 (MQ=255)                                                                                                         aCGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTa                              >  2:632269/1‑140 (MQ=255)                                                                                                          cGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTAt                             <  2:686073/140‑1 (MQ=255)                                                                                                          cGCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTAt                             <  2:460280/140‑1 (MQ=255)                                                                                                           gCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATc                            >  2:582509/1‑140 (MQ=255)                                                                                                           gCTCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATc                            <  2:568569/140‑1 (MQ=255)                                                                                                             tCAAACACGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATccc                          >  1:530084/1‑140 (MQ=255)                                                                                                                  cacGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGa                     <  2:801221/140‑1 (MQ=255)                                                                                                                   acGCGTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGac                    >  1:450697/1‑140 (MQ=255)                                                                                                                       gTATGAAAATGTGCCGATGCATCGGCGCAAATTCGGCGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCt                >  1:426358/1‑140 (MQ=255)                                                                                                                       gTATGAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCt                >  1:416412/1‑140 (MQ=255)                                                                                                                           gAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCttt                       >  1:176165/1‑129 (MQ=255)                                                                                                                           gAAAATGTGCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCttt                       <  2:176165/129‑1 (MQ=255)                                                                                                                              aaTGTGCCGATGCATCGCCGCATATTCGACGTAGCCGGCCTACATCCTAATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGt         <  2:225945/140‑1 (MQ=255)                                                                                                                                 gtgCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGATCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGcc      <  1:593503/140‑1 (MQ=255)                                                                                                                                 gtgCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGcc      >  2:718231/1‑140 (MQ=255)                                                                                                                                  tgCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACGACTGTCCCTTTGATACCTTTGCCGTGCCa     >  1:340643/1‑140 (MQ=255)                                                                                                                                   gCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATGTGCGCGATAACTATCCTTTTGACACCTTTGCCGTGCCaa    >  2:240090/1‑140 (MQ=255)                                                                                                                                   gCCGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCaa    <  1:793052/140‑1 (MQ=255)                                                                                                                                   gCCGATGCATCGGCGCAAA‑TCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAAt   <  1:423816/140‑1 (MQ=255)                                                                                                                                    ccGATGCATCGGCGCCAATTCGACGCAGCTGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAAt   <  1:300988/140‑1 (MQ=255)                                                                                                                                     cGATGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATg  <  2:450697/140‑1 (MQ=255)                                                                                                                                       aTGCATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTg             <  1:8163/127‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       CAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTGCAAACACAGCGTCTGAAATGGACGCTCAAACACGCGTATGAAAATGTGCCGATGTATCGGCGCAAATTCGACGCAGCAGGCGTACATCCTGATGATTTCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCCTTTGACACCTTTGCCGTGCCAATG  >  NC_000913/1462105‑1462376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑