Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,496,361	G→A	A358A (GCG→GCA)	ydcJ →	DUF1338 domain‑containing protein YdcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,496,361	0	G	A	100.0%	159.5 / NA	48	A358A (GCG→GCA)	ydcJ	DUF1338 domain‑containing protein YdcJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/27);  total (21/27)

CCGGGCAGGATAATCTCACTCACCAAATGCATTTACAGGAAACCTTCCGCACTTTTCCTGACAGTGAGTTTTTAATGCGTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCGCATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGAACGTGGTTGGGTAGTGGCGCAACCCATCACCTATGAAGATTTCTTGCCCGTTAGCGCGGCGGGGATCTTCCAGTCAAATCT  >  NC_000913/1496231‑1496489                                                                                                                                   |                                                                                                                                 ccGGGCAGGATAATCTTACTCACCAAATGCATTTACAGGAAACCTTCCGCACTTTTCCTGACAGTGAGTTTTTAATGCGTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAg                                                                                                                         <  2:497442/140‑1 (MQ=255) ccGGGCAGGATAATCTCACTCACCAAATGCATTTACAGGAAACCTTCCGCACTTTTCCTGACAGTGAGTTTTTAATGCGTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAg                                                                                                                         <  2:790911/140‑1 (MQ=255)       aGGATAATCTCACTCACCAAATGCATTTACAGGAAACCTTCCGCACTTTTCCTGACAGTGAGTTTTTAATGCGTCAGCAAGGGTTGGTATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGAtt                                                                                                                   <  1:687559/140‑1 (MQ=255)              tctcACTCACCAAATGCATTTACAGGAAACCTTCCGCACTTTTCCTGACAGTGAGTTTTTAATGCGTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTg                                                                                                            >  1:55903/1‑140 (MQ=255)                   ctcacCAAATGCATTTACAGGAAACCTTCCGCACTTTTCCTGACAGTGAGTTTTTAATGCGTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGAc                                                                                                       >  1:116835/1‑140 (MQ=255)                     cacCAAATGCATTTACAGGAAACCTTCCGCACTTTTCCTGACAGTGAGTTTTTAATGCGTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGa                                                                                                     >  2:814405/1‑140 (MQ=255)                        cAAATGCATTTACAGGAAACCTTCCGCACTTTTCCTGACAGTGAGTTTTTAATGCGTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATcc                                                                                                  >  1:831330/1‑140 (MQ=255)                         aaaTGCATTTACAGGGAACCTTCCGTACTGTTCCTGATAGTGAGTTTTTAATGCGTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCca                                                                                                 <  1:295438/140‑1 (MQ=255)                         aaaTGCATTTACAGGAAACCTTCCGCACTTTTCCTGACAGTGAGTTTTTAATGCGTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCca                                                                                                 <  2:116835/140‑1 (MQ=255)                         aaaTGCATTTACAGGAAACCTTACGCACTTTTCCTTACACTGAGTTTTAAATGCGTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCca                                                                                                 <  2:327166/140‑1 (MQ=255)                           aTGCATTTACAGGAAACCTTCCGCACTTTTCCTGACAGTGAGTTTTTAATGCGTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGA‑TCATCCTGGAGACGATCCACAg                                                                                              >  1:545740/1‑140 (MQ=255)                               aTTTACAGGAAACCTTCCGCACTTTTCCTGACAGTGAGTTTTTAATGCGTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCCACAGccc                                                                                           <  1:159822/140‑1 (MQ=255)                                tttACAGGAAACCTTCCGCACTTTTCCTGACAGTGAGTTTTTAATGCGTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCt                                                                                          >  1:764523/1‑140 (MQ=255)                                  tACAGGAAACCTTCCGCACTTTTCCTGACAGTGAGTTTTTAATGCGTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCTTCAGGCGTTTCGTCATGGAGACGATCCACAGCCCTTa                                                                                        >  1:375986/1‑140 (MQ=255)                                  tACAGGAAACCTTCCGCACTTTTCCTGACAGTGAGTTTTTAATGCGTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTa                                                                                        >  2:49496/1‑140 (MQ=255)                                       gAAACCTTCCGCACTTTTCCTGACAGTGAGTTTTTAATGCGTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGA‑TCATCCTGGAGACGATCCACAGCCCTTAATTGaa                                                                                  <  2:545740/140‑1 (MQ=255)                                        aaaCCTTCCGCACTTTTCCTGACAGTGAGTTTTTAATGCGTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGaa                                                                                  <  2:831330/140‑1 (MQ=255)                                         aaCCTTCCGCACTTTTCCTGACAGTGAGTTTTTAATGCGTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGaa                                                                                  <  1:145666/139‑1 (MQ=255)                                         aaCCTTCCGCACTTTTCCTGACAGTGAGTTTTTAATGCGTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGaa                                                                                  >  2:145666/1‑139 (MQ=255)                                          aCCTTCCGCACTTTTGCTGACATTGAGTTTTTAATGCTTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGAACg                                                                                <  1:290051/140‑1 (MQ=255)                                          aCCTTCCGCACTTTTCCTGACAGTGAGTTTTTAATGCGTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGAACg                                                                                <  2:800917/140‑1 (MQ=255)                                               ccGCACTTTTCCTGACAGTGAGTTTTTAATGCGTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGAACGTGGtt                                                                           <  1:790591/140‑1 (MQ=255)                                                     ttttCCTGACAGTGAGTTTTTAATGCGTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGAACGTGGTTGGGTAg                                                                     <  2:527640/140‑1 (MQ=255)                                                           tGACAGTGAGTTTTTAATGCGTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGAACGTGGTTGGGTAGTGgcgc                                                               <  2:55903/140‑1 (MQ=255)                                                           tGACAGTGAGTTTTTAATGCGTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGAACGTGGTTGGGTAGTGgcgc                                                               <  1:814405/140‑1 (MQ=255)                                                                 tGAGTTTTTAATGCGTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGAACGTGGTTGGGTAGTGgcgc                                                               >  1:35600/1‑134 (MQ=255)                                                                 tGAGTTTTTAATGCGTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGAACGTGGTTGGGTAGTGgcgc                                                               <  2:35600/134‑1 (MQ=255)                                                                 tGAGTTTTTAATGCGTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGAACGTGGTTGGGTAGTGGCGCAACCCa                                                         >  2:112629/1‑140 (MQ=255)                                                                 tGAGTTTTTAATGCGTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAAACGATCCAGAGCCCTTAATTGAACGGGGTTGGGTAGTGGCGCAACCCa                                                         >  2:275994/1‑140 (MQ=255)                                                                   aGTTTTTAATGCGTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGAACGTGGTTGGGTAGTGGCGCAACCCATc                                                       <  1:772003/140‑1 (MQ=255)                                                                    gTTTTTAATGCGTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGAACGTGGTTGGGTAGTGGCGCAACCCATCa                                                      >  1:496338/1‑140 (MQ=255)                                                                               gTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGAACGTGGTTGGGTAGTGGCGCAACCCATCACCTATGAAGAt                                           >  2:434505/1‑140 (MQ=255)                                                                                           tGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGAACGTGGTTGGGTAGTGGCGCAACCCATCACCTATGAAGATTTCTTGCCCGtt                               >  2:139940/1‑140 (MQ=255)                                                                                             gCATGGTTCCGGTACCGTCTGACGACTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGAACGTGGTTGGGTAGTGGCGCAACCCATCACCTATGAAGATTTCTTGCCCGTTAg                             >  2:544031/1‑140 (MQ=255)                                                                                             gCATGGTTCCGGTACCATCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGAACGTGGTTGGGTAGTGGCGCAACCCATCACCTATGAAGATTTCTTGCCCGTTAg                             <  2:496338/140‑1 (MQ=255)                                                                                               aTGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGAACGTGGTTGGGTAGTGGCGCAACCCATCACCTATGAAGATTTCTTGCCCGTTAgcg                           <  2:27498/140‑1 (MQ=255)                                                                                                 ggTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGAACGTGGTTGGGTAGTGGCGCAACCCATCACCTATGAAGATTTCTTGCCCGTTAGcgcg                         >  1:603915/1‑140 (MQ=255)                                                                                                  gTTCCGGTACCGTCTTACGCCTACGGGTGCGGCACATTGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGAACGTGGTTGGGTAGTGGCGCAACCCATCACCTATGAAGATTTCTTGCCCGTTAGCgcgg                        <  1:275994/140‑1 (MQ=255)                                                                                                  gTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGAACGTGGTTGGGTAGTGGCGCAACCCATCACCTATGAAGATTTCTTGCCCGTTAGCgcgg                        >  2:345075/1‑140 (MQ=255)                                                                                                            cGTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGAACGTGGTTGGGTAGTGGCGCAACCCATCACCTATGAAGATTTCTTGCCCGTTAGCGCGGCGGGGATCtt              <  1:544031/140‑1 (MQ=255)                                                                                                            cGTCTGACGCCTTCGGGTGAGGCACATCGCCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGAACGTGGTTGGGTAGTGGCGCAACCCATCACCTATGAAGATTTCTTGCCCGTTAGCGCGGCGGGGATCtt              <  1:542972/140‑1 (MQ=255)                                                                                                             gTCTGACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGAACGTGGTTGGGTAGTGGCGCAACCCATCACCTATGAAGATTTCTTGCCCGTTAGCGCGGCGGGGATCTTc             >  1:432878/1‑140 (MQ=255)                                                                                                                 gACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGAACGTGGTTGGGTAGTGGCGCAACCCATCACCTATGAAGATTTCTTGCCCGTTAGCGCGGCGGGGATCTTCCAGt         <  2:432878/140‑1 (MQ=255)                                                                                                                 gACGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGAACGTGGTTGGGTAGTGGCGCAACCCATCACCTATGAAGATTTCTTGCCCGTTAGCGCGGCGGGGATCTTCCAGt         <  2:603915/140‑1 (MQ=255)                                                                                                                   cGCCTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGAACGTGGTTGGGTAGTGGCGCAACCCATGACCTATGATGATCTCTGGCCCGTTAGCGCGTCGGGGGTCCTCCAGTCa       >  2:384647/1‑140 (MQ=255)                                                                                                                      cTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGAACGTGGTTGGGTAGTGGCGCAACCCATCACCTATGAAGATTTCTTGCCCGTTAGCGCGGCGGGGATCTTCCAGTCAAAt    <  1:112629/140‑1 (MQ=255)                                                                                                                      cTTCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGAACGTGGTTGGGTAGTGGCGCAACCCATCACCTATGAAGATTTCTTGCCCGTTAGCGCGGCGGGGATCTTCCAGTCAAAt    <  2:1207/140‑1 (MQ=255)                                                                                                                       ttCGGGTGAGGCACATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGAACGTGGTTGGGTAGTGGCGCAACCCATCACCTATGAAGATTTCTGGCCGGTTAGCGCTGCGGAGATCTTCCAGTCAAATc   >  2:303850/1‑140 (MQ=255)                                                                                                                        tCGGGTGACGCACATCCCCAGGCGATTCATCCTGGAGACCATCCACAGGCCTTAATTGAACGTGGTTGGGTAGTGGCGCAACCCATCACCTATGAAGATTTCTTGCCCGTTAGCGCGGCGGGGATCTTCCAGTCAAATCt  <  2:375986/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                 CCGGGCAGGATAATCTCACTCACCAAATGCATTTACAGGAAACCTTCCGCACTTTTCCTGACAGTGAGTTTTTAATGCGTCAGCAAGGGTTGGCATGGTTCCGGTACCGTCTGACGCCTTCGGGTGAGGCGCATCGTCAGGCGATTCATCCTGGAGACGATCCACAGCCCTTAATTGAACGTGGTTGGGTAGTGGCGCAACCCATCACCTATGAAGATTTCTTGCCCGTTAGCGCGGCGGGGATCTTCCAGTCAAATCT  >  NC_000913/1496231‑1496489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑