| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,518,660 | T→C | T63A (ACG→GCG) | mnaT ← | L‑amino acid N‑acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,518,660 | 0 | T | C | 97.2% | 112.9 / ‑4.5 | 37 | T63A (ACG→GCG) | mnaT | L‑amino acid N‑acyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (25/10); total (27/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
TCGGCTTAACAATTTACGCCCCAGACCTTTGCCCTGATGATCGGGATGGACATAAACCGAATGTTCCACGGTATGGCGAAAACCATCGAAACTACGCCAGTCGCCAAAC‑GAGGCATATCCCGTCACTACGCCGTTTTCCTCGCTCACCAGCACTGGATAACCTGCTAAAGTCCGCGCTTCAAACCAGGCAATGCGGTTATCAGCATCCACCGTTTGGTCATTCCAGATAGCCGCCGTATACAACACGGCGT > NC_000913/1518538‑1518788 | tcGGCTTAACAATTTACGCCCCAGCCCTTTGCCCTGATGAGCGGGAGGGACATAAACCGAATGTCCCACGGTATGGCGAAACCCATCGAAACTCCGCCAGTCGCCAAAC‑GAGGCATATCCCGCCACTACGCCGTTTTCCt < 2:512981/140‑1 (MQ=255)tcGGCTTAACAATTTACGCCCCAGACCTTTGCCCTGATGATCGGGATGGACATAAACCGAATGTTCCACGGTATGGCGAAAACCATCGAAATTACGCCAGCCGCTAAAC‑GAGGCATACCCCGGCACTACGCCGTTTTCCt > 2:374823/1‑140 (MQ=255) cTTAACAATTTACGCCCCAGACCTTTGCCCTGATGATCGGGATGGACATAAACCGAATGTTCCACGGTATGGCGAAAACCATCGAAACTACGCCAGTCGCCAGAC‑GAGGCGTATCCCGCCACTACGCCGTTTTCCTCGCt > 1:299728/1‑140 (MQ=255) aaCAATTTACGCCCCAGACCTTTGCCCTGATGATCGGGATGGACATAAACCGAATGTTCCACGGTATGGCGAAAACCATCGAAACTACGCCAGTCGCCAAAC‑GAGGCATATCCCGCCACTACGCCGTTTTCCTCGCTCAc > 1:11877/1‑140 (MQ=255) aCAATTTACGCCCCAGACCTTTGCCCTGATGATCGGGATGGACATAAACCGAATGTTCCACGGTATGGCGAAAACCATCGAAACTACGCCAGTCGCCAAAC‑GTGGCATATACCGCCAATACGCCGTTTTCCTCGCTCAcc > 1:282253/1‑140 (MQ=255) cccAGCCCTTTCCCCTGATGATCGGGATGGAAATAAACCGAATGTTCCAGGGTAGGGCGAAAACCATCGAAACTCCGCCGGCCGCAAAAC‑GAGGCATATCCCGCCACTACGCCGTTTTCCTCGCTCACCAGCACTGGATa < 2:783841/140‑1 (MQ=255) tGCCCTGATGATCGGGATGGACATAAACCGAATGTTCCACGGTATGGCGAAAACCATCGAAACTACGCCAGTCGCCAAAC‑GAGGCATATCCCGTCACTACGCCGTTTTCCTCGCTCACCAGCACTGGATAACCTGCTaaa > 2:766427/1‑140 (MQ=255) tGCCCTGATGATCGGGATGGACATAAACCGAATGTTCCACGGTATGGCGAAAACCATCGAAACTACGCCAGTCGCCAAAC‑GAGGCATATCCCGCCACTACGCCGTTTTCCTCGCTCACCAGCACTGGATAACCTGCTaaa > 1:155840/1‑140 (MQ=255) tGCCCTGATGATCGGGATGGACATAAACCGAATGTTCCACGGTATGGCGAAAACCATCGAAACTACGCCAGTCGCCAAAC‑GAGGCATATCCCGCCACTACGCCGTTTTCCTCGCTCACCAGCACTGGATAACCTGCTaaa > 1:682297/1‑140 (MQ=255) tGCCCTGATGATCGGGATGGACATAAACCGAATGTTCCACGGTATGGCGAAAACCATCGAAACTACGCCAGTCGCCAAAC‑GAGGCATATCCCGCCACTACGCCGTTTTCCTCGCTCACCAGCACTGGATAACCTGCTaaa > 1:529928/1‑140 (MQ=255) tGCCCTGATGATCGGGATGGACATAAACCGAATGTTCCACGGTATGGCGAAAACCATCGAAACTACGCCAGTCGCCAAAC‑GAGGCATATCCCGCCACTACGCCGTTTTCCTCGCTCACCAGCACTGGATAACCTGCTaaa > 1:449319/1‑140 (MQ=255) atgatCGGGATGGACATAAACCGAATGTTCCACGGTATGGCGAAAACCATCGAAACTACGCCAGTCGCCAAAC‑GAGGCATATCCCGCCACTACGCCGTTTTCGTCGCTCACCAGCACTGGATAATCTGCTAAAGTCcgcg > 1:318998/1‑140 (MQ=255) atgatCGGGATGGACATAAACCGAATGTTCCACGGTATGGCGAAAACCATCGAAACTACGCCAGTCGCCAAAC‑GAGGCATATCCCGCCACTACGCCGTTTTCGTCGCTCACCAGCACAGGATAACCTGCTAAAGTCcgcg > 1:351664/1‑140 (MQ=255) atgatCGGGATGGACATAAACCGAATGTTCCACGGTATGGCGAAAACCATCGAAACTACGCCAGTCGCCAAAC‑GAGGCATATCCCGCCACTACGCCGTTTTCCTCGCTCACCAGCACTGGATAACCTGCTAAAGTCcgcg > 2:61774/1‑140 (MQ=255) atgatCGGGATGGACATAAACCGAATGTTCCACGGTATGGCGAAAACCATCGAAACTACGCCAGTCGCCAAAC‑GAGGCATATCCCGCCACTACGCCGTTTTCCTCGCTCACCAGCACTGGATAACCTGCTAAAGTCcgcg > 2:485642/1‑140 (MQ=255) ggggATGAACATAACCCGAATTTCCCACGGTATGGCAAAACCCACCAAAACTCCGCCAGTCCCAAACC‑GAGGCATATCCCGCCACTACGCCGTTTTCCTCGCTCACCAGCACTGGATAACCTGCTAAAGTCCGCGCTTCa < 2:155840/139‑1 (MQ=255) tGGACATAAACCGAATGTTCCACGGTATGGCGAAAACCATCGAAACTACGCCAGTCGCCAAAC‑GAGGCATATCCCGCCACTACGCCGTTTTTCTCGCTCAACAGGACTAGATAACCTGCTAAAGTCCGCGCTTCAAACCa > 2:265094/1‑140 (MQ=255) tGGACATAAACCGAATGTTCCACGGTATGGCGAAAACCATCGAAACTACGCCAGTCGCCAAAC‑GAGGCATATCCCGCCACTACGCCGTTTTCCTCGCTCACCAGCACTGGATAACCTGCTAAAGTCCGCGCTTCAAACCa > 1:477568/1‑140 (MQ=255) tGGACATAAACCGAATGTTCCACGGTATGGCGAAAACCATCGAAACTACGCCAGTCGCCAAAC‑GAGGCATATCCCGCCACTACGCCGTTTTCCTCGCTCACCAGCACTGGATAACCTGCTAAAGTCCGCGCTTCAAACCa > 2:571448/1‑140 (MQ=255) tGGACATAAACCGAATGTTCCACGGTATGGCGAAAACCATCGAAACTACGCCAGTCGCCAAAC‑GAGGCATATCCCGCCACTACGCCGTTTTCCTCGCTCACCAGCACTGGATAACCTGCTAAAGTCCGCGCTTCAAACCa > 2:480813/1‑140 (MQ=255) aaaaCCGAATGTTCCCGGGTAGGGAAAAACCCATCGAACCTCCCCCGGTCGCAAAAC‑GGGGCTTATCCCGCCACTACGCCGTTTTCCTCGCTCACCAGCACTGGATAACCTGCTAAAGTCCGCGCTTCAAACCAGGCAAt < 2:477568/139‑1 (MQ=255) aacccGAATGTCCCACGGTATGGCAAAACCCATCAAAACTCCGCCGGTCGCCAAAC‑GAGGCATATCCCGCCACTACGCCGTTTTCCTCGCTCACCAGCACTGGATAACCTGCTAAAGTCCGCGCTTCAAACCAGGCAATg < 2:557165/137‑1 (MQ=255) aCCGAATGTTCCACGGTATGGCGAAAACCATCGAAACTACGCCAGTCGCCAAAC‑GAGGCATATCCCGCCACTACGCCGTTTTCCTCGCTCACCAGCACTGGATAACCTGCTAAAGTCCGCGCTTCAAACCAGGCAATGCg > 2:175846/1‑140 (MQ=255) gAATGTTCCACGGTATGGCGAAAACCATCGAAACTACGCCAGTCGCCAAAC‑GAGGCATATCCCGCCACTACGCCGTTTTCCTCGCTCACCAGCACTGGATAACCTGCTAAAGTCCGCGCTTCAAACCAGGCAATGCGGtt > 1:177042/1‑140 (MQ=255) aaTGTTCCACGGTATGGCGAAAACCATCGAAACTACGCCAGTCGCCAAAC‑GAGGCATATCCCGCCACTACGCCGTTTTCCTCGCTCACCAGCACTGGATAACCTGCTAAAGTCCGCGCTTCAAACCAGGCAATGCGGTTa > 1:750434/1‑140 (MQ=255) aaTGTTCCACGGTATGGCGAAAACCATCGAAACTACGCCAGTCGCCAAAC‑GAGGCATATCCCGCCACTACGCCGTTTTCCTCGCTCACCAGCACTGGATAACCTGCTAAAGTCCGCGCTTCAAACCAGGCAATGCGGTTa > 1:557165/1‑140 (MQ=255) aCGGTATGGCGAAAACCATCGAAACTACGCCACTCGCCAAAC‑GAGGCATATCCCGCCACTACGCCGTTTTCCTCGCTCACCAGCACTGGATAACCTGCTAAAGTCCGCGCTTCAAACCAGGCAATGCGGTTATCAGCATc < 1:541360/140‑1 (MQ=255) tGGCGAAAACCATCGAAACTACGCCAGTCGCCAAAC‑GAGGCATATCCCGCCACTACGCCGTTTTCCTCGCTCACCAGCACTGGATAACCTGCTAAAGTCCGCGCTTCAAACCAGGCAATGCGGTTATCAGCATCCACCGt > 1:21907/1‑140 (MQ=255) ggCGAAAACCATCGAAACTACGCCAGTCGCCAAAC‑GAGGCATATCCCGCCACTACGCCGTTTTCCTCGCTCACCAGCACTGGATAACCTGCTAAAGTCCGCGCTTCAAACCAGGCAATGCGGTTATCAGCATCCACCGtt > 1:633302/1‑140 (MQ=255) aaacCCATCAAAACTCCGCCAGTCGCCAAAC‑GAGGCATACCCCGCCACTACGCCGTTTTCCTCGCTCACCAGCACTGGATAACCTGCTAAAGTCCGCGCTTCAAACCAGGCAATGCGGTTATCAGCATCCACCGTTTGGt < 2:682297/140‑1 (MQ=255) tCGAAACTACGCCAGTCGCCAAAC‑GAGGCATATCCCGCCACTACGCCGTTTTCCTCGCTCACCAGCACTGGATAACCTGCTAAAGTCCGCGTTTCAAACCTGGCGATGCGGTTATCACCATCGACCGTTTGGTCATTCCa > 2:380320/1‑140 (MQ=255) cGAAACTACGCCAGTCGCCAAAC‑GAGGCATATCCCGCCACTACGCCGTTTTCCTCGCTCACCAGCACTGGATAACCTGCTAAAGTCCGCGCGTCAAACCAGGCAGTGCAGTTATCAGAATCCGCCGTTTGGTCATTCCAg > 2:250191/1‑140 (MQ=255) cgcccAGCCCCCAACG‑GAGCCATTCCCCGCCACTACGCCGTTTTCCTCGCTCACCAGCACTGGATAACCTGCTAAAGTCCGCGCTTCAAACCAGGCAATGCGGTTATCAGCATCCACCGTTTGGTCATTCCAGATAgccg < 1:524755/137‑1 (MQ=255) aCGCCAGTCGCCAAAC‑GAGGCATATCCCGCCACTACGCCGTTTTCCTCGCTCACCAGCACTGGATAACCTGCTAAAGTCCGCGCTTCAAACCAGGCAATGCGGTTATCAGTATCAACGGTTTGGGCATTCCAGATAgccg > 1:239835/1‑140 (MQ=255) cccAGTCGCAAACG‑GAGGCATACCCCGCCACTACGCCGTTTTCCTCGCTCACCAGCACTGGATAACCTGCTAAAGTCCGCGCTTCAAACCAGGCAATGCGGTTATCAGCATCCACCGTTTGGTCATTCCAGATAgccgcc < 2:21907/139‑1 (MQ=255) ggccgcAAACGGAGGTATACCCTGCCACTGCGCCGTTGTCCTCGCTCACCAGCACTGGATAACCTGCTAAAGTCCGCGCTTCAAACCAGGCAATGCGGTTATCAGCATCCACCGTTTGGTCATTCCAGATAGCCGCCGta < 1:374823/135‑1 (MQ=255) gTCGCCAAAC‑GAGGCATATCCCGCCACTACGCCGTTTTCCTCGCTCACCAGCACTGGATAACCTGCTAAAGTCCGCGCTTCAAACCAGGCAATGCGGTTATCAGCATCCACCGTTTGGTCATTCCAGATAGCCGCCGtat > 2:21449/1‑140 (MQ=255) caaaaC‑GGGGCATACCCCGCCACTACGCCGTTTTCCTCGCTCACCAGCACTGGATAACCTGCTAAAGTCCGCGCTTCAAACCAGGCAATGCGGTTATCAGCATCCACCGTTTGGTCATTCCAGATAGCCGCCGTATacaa < 2:177042/138‑1 (MQ=255) taattctCCCGCCACGACGCCGATTGCCTTGCTCACCCGCACTGAATAACCTGCTAAAGTCCGCGCTTCAAACCAGGCAATGCGGTTATCAGCATCCACCGTTTGGTCATTCCAGATAGCCGCCGTATACAACACGGCGt < 1:380320/134‑1 (MQ=255) | TCGGCTTAACAATTTACGCCCCAGACCTTTGCCCTGATGATCGGGATGGACATAAACCGAATGTTCCACGGTATGGCGAAAACCATCGAAACTACGCCAGTCGCCAAAC‑GAGGCATATCCCGTCACTACGCCGTTTTCCTCGCTCACCAGCACTGGATAACCTGCTAAAGTCCGCGCTTCAAACCAGGCAATGCGGTTATCAGCATCCACCGTTTGGTCATTCCAGATAGCCGCCGTATACAACACGGCGT > NC_000913/1518538‑1518788 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |