Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,519,178	A→G	D51G (GAC→GGC)	curA →	NADPH‑dependent curcumin/dihydrocurcumin reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,519,178	0	A	G	100.0%	130.9 / NA	39	D51G (GAC→GGC)	curA	NADPH‑dependent curcumin/dihydrocurcumin reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/21);  total (18/21)

GCGTAATCGACGTTGGGTTCTGGCCTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTCGGCGGTACGGTGAGCCGTGTCGTGGAGTCGAATCATCCTGATTATCAGTCTGGCGACTGGGTG  >  NC_000913/1519044‑1519314                                                                                                                                       |                                                                                                                                         gcGTAATCAACGTTGGGTTCTGGCCTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGGCCCGt                                                                                                                                     <  2:180278/140‑1 (MQ=255)   gTAATCGACGTTGGGATCTGGCATCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGGCCCGtat                                                                                                                                   <  1:234345/140‑1 (MQ=255)    tAATCGACGTTGGGTTCTGGCCTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTATTACTGCGCACAGTCTATTTGACCCTGGGCCCGtata                                                                                                                                  >  1:384114/1‑140 (MQ=255)      aTCGACGTTGGGTTCTGGCCTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGGCCCGTATATg                                                                                                                                >  2:536353/1‑140 (MQ=255)            gTTGGGTTCTGGCCTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGGCCCGTATATGCGTGGa                                                                                                                          >  1:421648/1‑140 (MQ=255)               gggTTCTGGCCTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGGCCCGTATATGCGTGGACGt                                                                                                                       >  1:435746/1‑140 (MQ=255)                 gTTCTGGCCTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGGCCCGTATATGCGTGGACGTAt                                                                                                                     <  1:66688/140‑1 (MQ=255)                 gTTCTGGCCTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGGCCCGTATATGCGTGGACGTAt                                                                                                                     <  1:94983/140‑1 (MQ=255)                        ccTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGGCCCGTATATGCGTGGACGTATGAGCGAt                                                                                                              <  2:53720/140‑1 (MQ=255)                         cTCGCGTCCACATGGTGCACCTCTTCCGGCGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGGCCCGTATATGAGTGGACGTATGAGCGATg                                                                                                             <  1:230764/140‑1 (MQ=255)                          tCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGGCCCGTATATGCGTGGACGTATGAGCGATGa                                                                                                            >  1:195930/1‑140 (MQ=255)                           cgcgTCCACATGGCGTACCTGTCCCCGAGAATTTCCGACTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGGCCCGTATATGCGTGGACGTATGAGCGATGAg                                                                                                           <  1:242837/140‑1 (MQ=255)                           cgcgTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGGCCCGTATATGCGTGGACGTATGAGCGATGAg                                                                                                           <  2:567416/140‑1 (MQ=255)                           cgcgTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGGCCCGTATATGCGTGGACGTATGAGCGATGAg                                                                                                           <  1:536353/140‑1 (MQ=255)                            gcgTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGGCCCGTATATGCGTGGACGTATGAGCGATGAGc                                                                                                          >  1:412142/1‑140 (MQ=255)                                 cacaTGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGGCCCGTATATGCGTGGACGTATGAGCGATGAGCCATCt                                                                                                     <  1:675430/140‑1 (MQ=255)                                 cacaTGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGGCCCGTATATGCGTGGACGTATGAGCGATGAGCCATCt                                                                                                     >  2:675430/1‑140 (MQ=255)                                        cgcACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGGCCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCAc                                                                                              >  2:472584/1‑140 (MQ=255)                                         gcACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGGCCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCAcc                                                                                             <  1:472584/140‑1 (MQ=255)                                           aCCTGTTCCGGAGAACTTCCGTATTCAAGCAGATGATTTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGGCCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGc                                                                                           <  1:388482/140‑1 (MQ=255)                                            ccTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGGCCCGTATATGCGTGGACGTATGAGCGATGAg                                                                                                           <  1:41903/123‑1 (MQ=255)                                            ccTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGGCCCGTATATGCGTGGACGTATGAGCGATGAg                                                                                                           >  2:41903/1‑123 (MQ=255)                                                ttCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGGCCCGTATATGCGAGGACGGATGAGCGATGGGCCAGCTTATTCACCGCCTGtt                                                                                      >  2:388482/1‑140 (MQ=255)                                                  ccGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGGCCCGTGTATGCGTGGACGAATGTGCGATGAGGCATCTTATTCACCGCCTGTTGa                                                                                    >  2:216706/1‑140 (MQ=255)                                                         aTTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGGCCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTggc                                                                             <  2:195930/140‑1 (MQ=255)                                                                   tGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGGCCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATgg                                                                   <  1:760830/140‑1 (MQ=255)                                                                         agaTGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGGCCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGTGTGATGGTcggcg                                                             <  1:613140/140‑1 (MQ=255)                                                                                        acCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGGCCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTCGGCGGTACGGTGAGCCgtg                                              >  1:43932/1‑140 (MQ=255)                                                                                           gggTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGGCCCGTATATGCGTGGACGTATGAGAGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTCGGCGGTACGGTGAGCCGTGTCg                                           <  2:421648/140‑1 (MQ=255)                                                                                                   gACAGGTGTTACTGCGCACAGTTTAATTGCCCCTGGGTCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTCGGCGGTACGGTGAGCCGTGTCGTGGAGTCg                                   <  1:261030/140‑1 (MQ=255)                                                                                                      aGGTGTTACTGCGCACAGTTTATTTGTCCCTGGGCCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTCGGCGGTACGGTGAGCCGTGTCGTGGAGTCGAat                                >  2:617575/1‑140 (MQ=255)                                                                                                        gtgtTACTGCGCACAGTTTATTTGTCCCTGGGCCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTCGGCGGTACGGTGAGCCGTGTCGTGGAGTCGAatca                              <  1:127317/140‑1 (MQ=255)                                                                                                           ttACTGCGCACAGTTTATTTGTCCCTGGGCCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTCGGCGGTACGGTGAGCCGTGTCGTGGAGTCGAATCATcc                           >  2:564091/1‑140 (MQ=255)                                                                                                                  gcACAGTTTATTTGTCCCTGGGCCCGTATATGCGTGGTCGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTCGGCGGTACGGTGAGCCGTGTCGTGGAGTCGAAACATCCTGATTAt                    <  2:412142/140‑1 (MQ=255)                                                                                                                  gcACAGTTTATTTGTCCCTGGGCCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTCGGCGGTACGGTGAGCCGTGTCGTGGAGTCGAATCATCCTGATTAt                    <  1:564091/140‑1 (MQ=255)                                                                                                                  gcACAGTTTATTTGTCCCTGGGCCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTCGGCGGTACGGTGAGCCGTGTCGTGGAGTCGAATCATCCTGATTAt                    >  2:562408/1‑140 (MQ=255)                                                                                                                   cacaGTTTATTTGTCCCTGGGCCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTCGGCGGTACGGTGAGCCGTGTCGTGGAGTCGAATCATCCTGATTATc                   >  2:685984/1‑140 (MQ=255)                                                                                                                                tCCCTGGGCCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTCGGCGGTACGGTGAGCCGTGTCGTGGAGTCGAATCATCCTGATTATCAGTCTGGCGACTg      >  1:77062/1‑140 (MQ=255)                                                                                                                                tCCCTGGGCCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTCGGCGGTACGGTGAGCCGTGTCGTGGAGTCGAATCATCCTGATTATCAGTCTGGCGACTg      >  1:617993/1‑140 (MQ=255)                                                                                                                                  ccTGGGCCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTCGGCGGTACGGTGTGCCGTGTCGTGGCGTCAAATCATCCGGATTGTCAGTCTGGCGACTggg    >  2:282483/1‑140 (MQ=255)                                                                                                                                    tGGGCCCGTATATGCTTGGACGTATGAGCAATGAGCCCTCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTCGGCGGTACGGTGAGCCGTGTCGTGGAGTCGAATCATCCTGATTATCAGTCTGGCGACTGGGTg  <  1:238364/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         GCGTAATCGACGTTGGGTTCTGGCCTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTCGGCGGTACGGTGAGCCGTGTCGTGGAGTCGAATCATCCTGATTATCAGTCTGGCGACTGGGTG  >  NC_000913/1519044‑1519314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑