Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,552,034	C→T	intergenic (+43/+364)	fdnI → / ← yddM	formate dehydrogenase N subunit gamma/putative DNA‑binding transcriptional regulator YddM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,552,034	0	C	T	100.0%	133.3 / NA	40	intergenic (+43/+364)	fdnI/yddM	formate dehydrogenase N subunit gamma/putative DNA‑binding transcriptional regulator YddM
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/26);  total (14/26)

GGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGC  >  NC_000913/1551898‑1552144                                                                                                                                         |                                                                                                               ggTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGTGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCAt                                                                                                             <  1:691724/140‑1 (MQ=255) ggTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCAt                                                                                                             <  2:766121/140‑1 (MQ=255)     aGTCGTCGCTGGGCGAAGAAACACCGTCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTc                                                                                                         <  1:399052/140‑1 (MQ=255)     aGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTc                                                                                                         <  2:748644/140‑1 (MQ=255)     aGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTc                                                                                                         <  2:713279/140‑1 (MQ=255)     aGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTc                                                                                                         <  1:804075/140‑1 (MQ=255)     aGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTc                                                                                                         <  1:53160/140‑1 (MQ=255)     aGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTc                                                                                                         <  1:413979/140‑1 (MQ=255)     aGTCGTCGCTGGGCGAACAAACACCATCCGCTCTGGTATGGTGAAATCTAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTc                                                                                                         <  2:256084/140‑1 (MQ=255)          tcgCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTCATAtt                                                                                                    <  1:122534/140‑1 (MQ=255)            gCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTCATAtt                                                                                                    <  1:520060/138‑1 (MQ=255)            gCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTCATAtt                                                                                                    >  2:520060/1‑138 (MQ=255)               gggCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGa                                                                                               <  1:623223/140‑1 (MQ=255)                ggCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGAc                                                                                              <  2:798092/140‑1 (MQ=255)                 gCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAATTGGCGCTGTAAAAGGCGTCATATTCATATTGTAGACa                                                                                             >  1:396969/1‑140 (MQ=255)                    aagaaACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACg                                                                                          <  2:657810/140‑1 (MQ=255)                          cacCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCt                                                                                    <  2:558937/140‑1 (MQ=255)                               tCCGCGCTGGTATCGTGTAATCTAGAAGGCATAAGCGAAAAAAGAGAGAGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGtt                                                                               <  2:320754/140‑1 (MQ=37)                                ccGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGAGCTGTAAAAGGCTTCATCTTCATATGGTAGCGAACGTAGGCTTTGTTc                                                                              >  2:387201/1‑140 (MQ=255)                                 cgcgCTGGTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCa                                                                             >  1:755738/1‑140 (MQ=255)                                         tATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGAt                                                                     >  1:119480/1‑140 (MQ=255)                                                  aTCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGa                                                            <  2:473171/140‑1 (MQ=255)                                                         aGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCtt                                                     >  1:160105/1‑140 (MQ=255)                                                         aGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCtt                                                     >  1:703669/1‑140 (MQ=255)                                                               aaGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCgg                                               <  2:119480/140‑1 (MQ=255)                                                                            gagTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGTATGCGGCTTGAACGCCTTATCCGGCATGAAAACCCtt                                  >  1:322807/1‑140 (MQ=255)                                                                                gaagaaGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCaaa                              <  2:755738/140‑1 (MQ=255)                                                                                       ggATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGGACGCCTTGTCCGGCATGAAAACCCTTCAAATCCAATa                       >  1:288940/1‑140 (MQ=255)                                                                                       ggATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGaa                                         <  2:160782/122‑1 (MQ=255)                                                                                       ggATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGaa                                         >  1:160782/1‑122 (MQ=255)                                                                                                 tctTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTg             >  1:76969/1‑140 (MQ=255)                                                                                                 tctTTTGAACTTTAAGCAGAAAATGGCGCTGTAAACGGCCTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTg             <  2:322807/140‑1 (MQ=255)                                                                                                  ctTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGa            >  1:438256/1‑140 (MQ=255)                                                                                                     ttGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGAATGAAAACCCTACAGATCCAATAGATTGCAGTGAACg         >  1:316129/1‑140 (MQ=255)                                                                                                       gAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATACCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACgtg       <  1:432380/140‑1 (MQ=255)                                                                                                        aaCTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATACCGGATGCGGCGTGAACGCCTTATCCAGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACgtg       <  1:806368/139‑1 (MQ=255)                                                                                                        aaCTTTAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATACCGGATGCGGCGTGAACGCCTTATCCAGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACgtg       >  2:806368/1‑139 (MQ=255)                                                                                                         aCTTTAAGCTGAAAATGGCGCTATAGAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTa     <  1:387201/140‑1 (MQ=255)                                                                                                            ttAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGc  <  2:703669/140‑1 (MQ=21)                                                                                                            ttAAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGc  <  2:160105/140‑1 (MQ=21)                                                                                                                                         |                                                                                                               GGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGC  >  NC_000913/1551898‑1552144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑