Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,560,788	C→T	A48T (GCT→ACT)	ddpB ←	putative D,D‑dipeptide ABC transporter membrane subunit DdpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,560,788	0	C	T	100.0%	65.7 / NA	25	A48T (GCT→ACT)	ddpB	putative D,D‑dipeptide ABC transporter membrane subunit DdpB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/1);  total (24/1)

TCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGA  >  NC_000913/1560655‑1560922                                                                                                                                      |                                                                                                                                       tctTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGTCTGGTCCAATCCGAGTCGCTGGCGATTATTTTCCACGATAGTATctgt                                                                                                                                  >  2:310176/1‑137 (MQ=255)     ccAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGTATCGCTGgcg                                                                                                                              >  2:184577/1‑140 (MQ=255)             ggACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGTATCGCTGGCGCGGTCAcc                                                                                                                      >  1:465295/1‑140 (MQ=255)              gACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCTAATATTTTCCATGAGAGTATCACTGGCGCGGTCAccc                                                                                                                     >  1:388037/1‑140 (MQ=255)              gACGCCCGGTACGAAGGTATGTCCCCAGGTCGCCAAGAATCAGGTCGCTGACGTAGCGGTAAAACTTTACATACAGGGGCGGGTCCAGCCCCGGTTGCTGGCAAATATTTCCCACGATAGTGTCGCTGGCGCGGcccccc                                                                                                                     <  2:267312/140‑1 (MQ=255)                      gTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCCCCATATTATCACTAGCGCGGTCACCCCCCAGTaa                                                                                                             >  2:824810/1‑140 (MQ=255)                          gAATGGATGTCCCCAGGTCACCAGAAAACGGGTCGCGGAGGTAGGGAAAAAACTGTACATACGGTGGCTGGTCCAGCCCCAGTTGCTGGCGAATTTTTTCCACGATAGTATCGCGGGCGCGGTCACCCGCCAGTAACcgc                                                                                                         <  2:608238/140‑1 (MQ=255)                           aaTGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGTATCGCTGGCGCGGTCACCCGCCAGTAACcgcg                                                                                                        >  1:608238/1‑140 (MQ=255)                             tGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGTATCGCTGGCGCGGTGACCCGCCAGGAACCGGGcc                                                                                                      >  1:455117/1‑140 (MQ=255)                               gATGTCCCCAGGTCACCATGAAACGGGTCGCTGACGTGGGGGAAAAACTGTACATAGGGGGGCTGGCCCAGCCCCGGTGGCGGGCGAATTTTTCCCACGATAGTATCGCGGGCGCGGTCCCCCGCCAGTAACCGCGCCgg                                                                                                    <  2:707695/140‑1 (MQ=255)                                 tGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGTGTCGCTGGAGCGGTCACCCGCCAGTAACCGCGCCGGAt                                                                                                  >  1:258047/1‑140 (MQ=255)                                 tGTCCCCAGGCCACCATAAAACCGGTCGCTGGCGTAGCGCTAAACCTGTACATAAAGTGGCTGGCCCGGTCCCAGTTGCGGGCAAATATTTCCCACGATAGTATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGAt                                                                                                  <  2:258047/140‑1 (MQ=255)                                    tCCCAGGTCACCATGAACCGGGTCGCTGACGTAGCGGAAAAACTGTACATACAGGGGCTGGTCCAGCCCCAGTTGCGGGCGAATTTTTTCCACGATAGTATCGCTGGCGGGGTCCCCCGCCAGTAACCGCGCCGGATCAc                                                                                               <  2:645965/140‑1 (MQ=255)                                     cccAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGTATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCAcc                                                                                              >  1:8731/1‑140 (MQ=255)                                      ccgggTCCCCAGGAAACAGGGCGCTGCCGTAGCGGAAAAACTTTCCATAGAGTGGCGGGTCCGGCCCCAGTTGGTGGCTAATTTTTTCCACGATAGTATCGCTGGCGGGGTCACCCGCCAGTAACCGCGCCGGATCACCa                                                                                             <  2:465295/137‑1 (MQ=255)                                               cATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGTATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGa                                                                                    >  2:731515/1‑140 (MQ=255)                                                 tGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGCCCCAGTTGCGGGCGAATTTTTTCCACGATAGTATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATg                                                                                  <  1:184577/140‑1 (MQ=255)                                                         gTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGTATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGGTCACCAGGGATCAGATGCGGGataa                                                                          >  1:735467/1‑140 (MQ=255)                                                         gTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGTATCGCTGGCGCGGTCACCCGCCAGAAACCGCGCCGGATCGCCAGGGGTCAGATGCGAGataa                                                                          >  1:317608/1‑140 (MQ=255)                                                                           agaCTGTAAATACAGGGTCTGCTCCAGCCCCGGTTGCTGCCAAATTTTTCCCACGATAGTATCGCGGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGa                                                        <  2:278495/138‑1 (MQ=255)                                                                                  acatacaGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGTATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCTGATGCGAGATAATAAACGTAAGCACGCAGAcgcccgc                                                 >  1:245484/1‑140 (MQ=255)                                                                                   agtccaGTGGCTGGCCCAGCCCGTGTTGCTGGCAAATTTTTCCCACGATAGTATCGCGGGCGCGGTCCCCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGAcgcccgcc                                                <  2:739235/136‑1 (MQ=255)                                                                                        aGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGTATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCAAGATAATTAACGTACTCACGCAGACGCCCGccaccct                                           >  1:278495/1‑138 (MQ=255)                                                                                           ggCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGTATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAg                                        >  1:615150/1‑140 (MQ=255)                                                                                           ggCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGTATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGAACAGATGCGCGATAATAGACGTAATCACGCAAACGCCCGCCACCACGAg                                        >  2:358968/1‑140 (MQ=255)                                                                                           ggCTGGCCCAATCCCCGTTGCTGGCGAATATTTTCCACGATAGTATCGCTGGCGCCGGCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAg                                        >  2:561143/1‑140 (MQ=255)                                                                                            gCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGTATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGc                                       >  2:153995/1‑140 (MQ=255)                                                                                             cTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGTATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGGTCTGATGCGAGGTAATAAATGTAATCACGCAGAGGCCCGCCACCACGAGCa                                      >  2:227217/1‑140 (MQ=255)                                                                                             cTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGTATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCa                                      >  1:432809/1‑140 (MQ=255)                                                                                               ggTCCAGTCCCAGTTGCTGGGGAATATTTTCCACGATAGTATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCAcc                                    >  2:482902/1‑140 (MQ=255)                                                                                                gcccAGCCTCAGTGGCCGGGGAATTTTTCCGTCTAGAGTATCGCGGGCAGGGCCCCCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCa                                   <  2:388037/138‑1 (MQ=255)                                                                                                gTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGTATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCAcct                                   >  1:739235/1‑139 (MQ=255)                                                                                                gTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGTATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCa                                   >  2:435063/1‑140 (MQ=255)                                                                                                           gTTGCTGGCGAATATTTTCCACGATAGTATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATGAACGTAATCACGTAGATGCCCGCCAGCACGCGCACCAGCCCCcagcag                        >  2:335950/1‑140 (MQ=255)                                                                                                           gTTGCTGGCGAATATTTTCCACGATAGTATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCcagcag                        >  1:170068/1‑140 (MQ=255)                                                                                                           gTTGCTGGCGAATATTTTCCACGATAGTATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCcagcag                        >  2:654558/1‑140 (MQ=255)                                                                                                             tGCGGGCAAATTTTTTCCACGATAGTATCACGGGCGGCGTCCCCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCg                      <  1:358968/140‑1 (MQ=255)                                                                                                                tGGCGAATATTTTCCACGATAGTATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTg                   >  1:153555/1‑140 (MQ=255)                                                                                                                   agAATATTTTCCCCGATATTCTCGCTGGGTCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCg                <  1:824810/139‑1 (MQ=255)                                                                                                                      atatTTTCCACGATAGTGTCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTaa             >  2:709282/1‑140 (MQ=255)                                                                                                                                 gATAGTATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGa  >  1:796204/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                       TCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGA  >  NC_000913/1560655‑1560922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑