| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,606,758 | T→C | V220A (GTA→GCA) | lsrF → | 3‑hydroxy‑2,4‑pentadione 5‑phosphate thiolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,606,758 | 0 | T | C | 95.7% | 148.6 / ‑4.6 | 46 | V220A (GTA→GCA) | lsrF | 3‑hydroxy‑2,4‑pentadione 5‑phosphate thiolase |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (20/24); total (21/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGCGTAATATTTTCCAGTCTGACCATCCGGTGGCGA > NC_000913/1606623‑1606892 | gCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTAGTATGTCGAGAAAAGTTGTGAACGGATTGTTGCCGGATGTCCGGCAccc > 2:221073/1‑140 (MQ=255) ggCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGt > 1:117748/1‑140 (MQ=255) aGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTg > 2:814176/1‑140 (MQ=255) aGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTg > 2:789153/1‑140 (MQ=255) ggTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCg > 2:700130/1‑140 (MQ=255) cgATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTaaaa > 1:490820/1‑140 (MQ=255) tCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAAt > 1:317192/1‑140 (MQ=255) cGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTACCCATTGTTTTTGCTTGCGGTAAAAAATTAcc > 2:355846/1‑140 (MQ=255) aTTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAg > 1:51221/1‑140 (MQ=255) ctcgctcgCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGcgcg < 2:51221/140‑1 (MQ=255) cgctcgcGACTCGAATCGCCGCTGAGATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGa > 2:559915/1‑139 (MQ=255) cgctcgcGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGa < 1:559915/139‑1 (MQ=255) cgctcgcGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAg > 2:91356/1‑140 (MQ=255) cgcgACTCGAATCGCCGTTGAATTGGGGGCGTAAATTATTAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGgcgc < 2:209678/140‑1 (MQ=255) cgACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTg > 2:739158/1‑140 (MQ=255) aCTCGAATCGCCGCTTAAATGGTGGAGCATATTATCATAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGa < 1:221073/140‑1 (MQ=255) aaTCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAAtgt > 2:485595/1‑140 (MQ=255) ccgcTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTgg < 1:814176/140‑1 (MQ=255) gcTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCa < 1:91356/140‑1 (MQ=255) gcTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCa < 1:700130/140‑1 (MQ=255) gcTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCa < 1:2008/140‑1 (MQ=255) tGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAgg < 1:789153/140‑1 (MQ=255) tGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAgg < 1:485595/140‑1 (MQ=255) aaaTGGGGGCGCAAAATATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGTGCGAGGCGCTGGAAATGTGCTGGCAGGCt < 1:355846/140‑1 (MQ=255) ggggCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTa > 1:179551/1‑136 (MQ=255) ggggCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTa < 2:179551/136‑1 (MQ=255) gggCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGAt < 1:596319/140‑1 (MQ=255) gcAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCa < 1:65591/126‑1 (MQ=255) gcAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCa > 2:65591/1‑126 (MQ=255) cAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTg < 2:490820/140‑1 (MQ=255) aaaaCCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTgg < 2:148103/140‑1 (MQ=255) aaCCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGgtg < 2:317192/140‑1 (MQ=255) aaCCTATTATGTCGAAAAAGGTTTTGAACGGATAGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGgtg < 2:348504/140‑1 (MQ=255) ccTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGtgtg > 1:143615/1‑140 (MQ=255) tattatGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGa > 1:35490/1‑140 (MQ=255) attatGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGat > 1:811764/1‑140 (MQ=255) ttatGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGata < 1:460731/140‑1 (MQ=255) tCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATgggg < 2:117748/140‑1 (MQ=255) aaGGTTTTGAACGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGCGTAat < 2:143615/140‑1 (MQ=255) aCGGATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGCGTAATATTTTCCAGt < 1:739158/140‑1 (MQ=255) gATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGCg > 1:649243/1‑123 (MQ=255) gATTGTTGCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGCg < 2:649243/123‑1 (MQ=255) ttgCCGGATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGCGTAATATTTTCCAGTCTGACCAt < 2:476101/140‑1 (MQ=255) gATGTCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGGTATGGGGGGTAATAGTTTCCAGTCTGACCATCCGGTg > 1:247061/1‑140 (MQ=255) aTGTCCGGCACCCATTGATATTCCTGGCGGTAAAACATTCCCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGCGTAATATTTTCCAGTCTGACCATCCGGTgg < 2:233869/140‑1 (MQ=255) tCCGGCACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGCGTAATATGTTGCAGTCTGACCATCCGGTGGCga > 1:233869/1‑140 (MQ=255) | GCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGCGTAATATTTTCCAGTCTGACCATCCGGTGGCGA > NC_000913/1606623‑1606892 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |