Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,622,793	A→G	intergenic (+52/+24)	mgtS → / ← mgrR	small protein MgtS/small regulatory RNA MgrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,622,793	0	A	G	100.0%	68.3 / NA	20	intergenic (+52/+24)	mgtS/mgrR	small protein MgtS/small regulatory RNA MgrR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/0);  total (20/0)

CCGTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCCTTGTTACAGTTGTGTACAAGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACA  >  NC_000913/1622674‑1622923                                                                                                                        |                                                                                                                                   ccGTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTCACCGGCCCCTTGGTACAGTTGTGTACAGGGGGCCTGATTTTTATGACg                                                                                                                >  2:400793/1‑140 (MQ=255)      cTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCCTTGTTACAGTTGTGTACAGGGGGCCTGATTTTTATGACGGCGaa                                                                                                           >  1:732382/1‑140 (MQ=255)                                             ttCAGCCACAAGTGGGATAACTAAGGAAGGGAGATATTCCCTCCCCTACCCCGCCCTTTGTTACGGTTGTGTACGGGGGGCTTTTTTTTTATGGCGGAAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATgg                                                                    <  2:732382/140‑1 (MQ=255)                                                                     tGAACGGAGATAATCCCTCACCTAACCGGCCCCTTGTTACAGTTGTGTACAGGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACg                                            >  1:70505/1‑140 (MQ=255)                                                                            agaTAATCCCTCACCTAACCGGCCCCTTGTTACAGTTGTGTACAGGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAg                                     >  2:782060/1‑140 (MQ=255)                                                                              aTAATCCCTCACCTAACCGGCCCCTTGTTACAGTTGTGTACAGGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATTGATAGATTTGTGTTTTGCTTTTACGCTAACAGGc                                   >  1:120898/1‑140 (MQ=255)                                                                              aTAATCCCTCACCTAACCGGCCCCTTGTTACAGTTGTGTACAGGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGc                                   >  2:78019/1‑140 (MQ=255)                                                                                      tCACCTAACCGGCCCCTTGTTACAGTTGTGTACAGGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCt                           >  1:591354/1‑140 (MQ=255)                                                                                      tCACCTAACCGGCCCCTTGTTACAGTTGTGTACAGGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCt                           >  1:113220/1‑140 (MQ=255)                                                                                      tCACCTAACCGGCCCCTTGTTACAGTTGTGTACAGGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTGTTACGCTAACAGGCATTTTCCt                           >  1:323236/1‑140 (MQ=255)                                                                                       cACCTAACCGGCCCCTTGTTACAGTTGTGTACAGGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAAAAGGCATTTTCCTg                          >  1:646240/1‑140 (MQ=255)                                                                                        aCCTAACCGGCCCCTTGTTACAGTTGTGTACAGGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGc                         >  2:125416/1‑140 (MQ=255)                                                                                        aCCTAACCGGCCCCTTGTTACAGTTGTGTACAGGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGc                         >  1:129309/1‑140 (MQ=255)                                                                                            aaCCGGCCCCTTGTTACAGTTGTGTACAGGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTccctgtctc                     >  1:758390/1‑133 (MQ=255)                                                                                            aaCCGGCCCCTTGTTACAGTTGTGTACAGGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTg                     >  1:594750/1‑140 (MQ=255)                                                                                             aCCGGCCCCTTGTTACAGTTGTGTACAGGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTTTTTTGCTGTTAAGCAAACAGGGATTTTCCTGCACTGa                    >  1:233842/1‑140 (MQ=255)                                                                                             aCCGGCCCCTTGTTACAGTTGTGTACAGGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGa                    >  1:719372/1‑140 (MQ=255)                                                                                             aCCGGCCCCTTGTTACAGTTGTGTACAGGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTAAGCAAACAGGAATTTTCCTGCAcctg                    >  1:349755/1‑137 (MQ=255)                                                                                                  ccccTTGTTACAGTTGTGTACAGGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACg               >  1:589199/1‑140 (MQ=255)                                                                                                    ccTTGTTACAGTTGTGTACAGGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGaa             >  2:674445/1‑140 (MQ=255)                                                                                                               ttGTGTACAGGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGAcaca  >  1:37242/1‑140 (MQ=255)                                                                                                                        |                                                                                                                                   CCGTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCCTTGTTACAGTTGTGTACAAGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACA  >  NC_000913/1622674‑1622923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑