Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,644,058	A→G	S83P (TCC→CCC)	ydfU ←	Qin prophage; protein YdfU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,644,058	0	A	G	100.0%	185.0 / NA	53	S83P (TCC→CCC)	ydfU	Qin prophage; protein YdfU
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/19);  total (34/19)

TGGTCACGCAGTTGGTTGTCGCAGTGCCAGCACAACACCATTGCGCCGGTACCATAACGGTGAATGACTGTTTCAGTGTGATGGTAATCGCCATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGACAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAGCAATGTTTTGTCTTCCGCCAGCGGCTGGCGAACGGCAGGAACGACTCCGGATGGCAGATTACGCATGCTTTTTGGTT  >  NC_000913/1643922‑1644192                                                                                                                                         |                                                                                                                                       tGGTCACGCAGTTGGTTGTCGCAGTGCCAGCACAACACCATTGCGCCGGTACCATAACGGTGAATGACTGTTTCAGTGTGATGGTAATCGCCATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCaa                                                                                                                                     >  1:764629/1‑140 (MQ=255)                      cAGTGCCAGCACAACACCATTGCGCCGGTACCATAACGGTGAATGACTGTTTCAGTGTGATGGTAATCGCCATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCagcagc                                                                                                                >  1:540016/1‑140 (MQ=255)                      cAGTGCCAGCACAACACCATTGCGCCGGTACCATAACGGTGAATGACTGTTTCAGTGTGATGGTAATCGCCATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCagcagc                                                                                                                >  2:634255/1‑140 (MQ=255)                       aGTGCCAGCACAACACCATTGCGCCGGTACCATAACGGTGAATGACTGTTTCAGTGTGATGGTAATCGCCATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAgcagca                                                                                                               <  1:183029/140‑1 (MQ=255)                            cAGCACAACACCATTGCGCCGGTACCATAACGGTGAATGACTGTTTCAGTGTGATGGTAATCGCCATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAAt                                                                                                          <  2:824104/140‑1 (MQ=255)                             aGCACAACACCATTGCGCCGGTACCATAACGGTGAATGACTGTTTCAGTGTGATGGTAATCGCCATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCGATGAGCAAACGCCGCCAGCAGCACGAATc                                                                                                         >  1:308068/1‑140 (MQ=255)                               cacaACACCATTGCGCCGGTACCATAACGGTGAATGACTGTTTCAGTGTGATGGTAATCGCCATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCAc                                                                                                       <  2:659373/140‑1 (MQ=255)                                     acCATTGCGCCGGTACCATAACGGTGAATGACTGTTTCAGTGTGATGGTAATCGCCATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCCATGCGCCAACGTCGCCAGCAGCACGAATCACCcgttc                                                                                                 >  1:372954/1‑140 (MQ=255)                                       cATTGCGCCGGTACCATAACGGTGAATGACTGTTTCAGTGTGATGGTAATCGCCATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCcgttcgt                                                                                               <  2:540016/140‑1 (MQ=255)                                          tGCGCCGGTACCATAACGGTGAATGACTGTTTCAGTGTGATGGTAATCGCCATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTAc                                                                                            <  1:429907/140‑1 (MQ=255)                                                 gTACCATAACGGTGAATGACTGTTTCAGTGTGATGGTAATCGCCATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGGCGCCAGCCGCACGAATCACCCCTTCGTTACTaaaaaa                                                                                     >  1:403049/1‑140 (MQ=255)                                                 gTACCATAACGGTGAATGACTGTTTCAGTGTGATGGTAATCGCCATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCAc                                                                                                       <  1:316639/122‑1 (MQ=255)                                                 gTACCATAACGGTGAATGACTGTTTCAGTGTGATGGTAATCGCCATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCAc                                                                                                       >  2:316639/1‑122 (MQ=255)                                                       tAACGGTGAATGACTGTTTCAGTGTGATGGTAATCGCCATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGgcag                                                                               <  1:428388/140‑1 (MQ=255)                                                         aCGGTGAATGACTGTTTCAGTGTGATGGTAATCGCCATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGgcagca                                                                             <  1:494868/140‑1 (MQ=255)                                                           ggTGAATGACTGTTTCAGTGTGATGGTAATCGCCATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGgcagc                                                                              >  1:783925/1‑137 (MQ=255)                                                           ggTGAATGACTGTTTCAGTGTGATGGTAATCGCCATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGgcagc                                                                              <  2:783925/137‑1 (MQ=255)                                                             tGAATGACTGTTTCAGTGTGATGGTAATCGCCATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCTAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAGCAATGt                                                                         <  1:557742/140‑1 (MQ=255)                                                               aaTGACTGTTTCAGTGTGATGGTAATCGCCATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACgg                                                                                  <  2:764892/129‑1 (MQ=255)                                                               aaTGACTGTTTCAGTGTGATGGTAATCGCCATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACgg                                                                                  >  1:764892/1‑129 (MQ=255)                                                               aaTGACTGTTTCAGTGTGATGGTAATCGCCATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAGCAATGttt                                                                       >  2:483663/1‑140 (MQ=255)                                                               aaTGACTGTTTCAGTGTGATGGTAATCGCCATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAGCAATGttt                                                                       <  2:817312/140‑1 (MQ=255)                                                                     tGTTTCAGTGTGATGGTAATCGCCATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACACGTTCGTTACTGAATAACGGCTGCAATGTTTTGTCtt                                                                 >  1:375840/1‑140 (MQ=255)                                                                       tttCAGTGTGATGGTAATCGCCATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTATTAAAAACCGGCAGTAATGTTTTGTCTTcc                                                               >  1:269714/1‑140 (MQ=255)                                                                       tttCAGTGTGATGGTAATCGCCATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAGCAATGTTTTGTCTTcc                                                               >  2:148750/1‑140 (MQ=255)                                                                       tttCAGTGTGATGGTAATCGCCATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCATTCGTTAGTAATAACCGGCAGTAATGTTTTGTCTTcc                                                               >  1:251948/1‑140 (MQ=255)                                                                             tgtgATGGTAATCGCCATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTGACTAAAACACGGCACCAATGTTTTGTCTTCCGCCAGc                                                         >  1:325548/1‑140 (MQ=255)                                                                                 aTGGTAATCGCCATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCGGTTCGTTACTAAAAAATGGCAGCAAGGTTTAGTCTTCCGCCAGCGGCt                                                     >  2:226445/1‑140 (MQ=255)                                                                                 aTGGTAATCGCCATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAGCAATGTTTTGTCTTCCGCCAGCGGCt                                                     >  2:139086/1‑140 (MQ=255)                                                                                      aaTCGCCATTAGGCCACGGGCATGATGTAACCTGATGTAGTAGCCAGTTGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAGCAATGTTTTGTCTTCCGCCAGCGGCTGGCGa                                                <  2:308068/140‑1 (MQ=255)                                                                                         cGCCATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAGCAATGTTTTGTCTTCCGCCAGCGGCTGGCGAACg                                             >  1:808990/1‑140 (MQ=255)                                                                                           ccATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAGCAATGGTTTGCCTTCCGCCAGCTGCTGGCGAACGGc                                           >  2:474856/1‑140 (MQ=255)                                                                                              ttAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAGCAATGTTTTGTCTTCCGCCAGCGGCTGGCGAACGGCAgg                                        >  1:124871/1‑140 (MQ=255)                                                                                                aGGCCACTGGCAGGATGAAACAAGACGTAATAGCCTGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAGCAATGTTTTGTCTTCCGCCAGCGGCTGGCGAACGGCAGGaa                                      <  2:251948/140‑1 (MQ=255)                                                                                                      cTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAGCAATGTTTTGTCTTCCGCCAGCGGCTGGCGAACGGCAGGAACGACTc                                >  1:85911/1‑140 (MQ=255)                                                                                                      cTGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAGCAATGTTTTGTCTTCCGCCAGCGGCTGGCGAACGGCAGGAACGACTc                                >  2:42810/1‑140 (MQ=255)                                                                                                       tGGCAGGATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAGCAATGTTTTGTCTTCCGCAAGCCGCGGGCGAAAGGCAGGAACGACTcc                               >  1:397353/1‑140 (MQ=255)                                                                                                          cAGGATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAGCAATGTTTTGTCTTCCGCCAGCGGCTGGCGAACGGCAGGAACGACTCCGGa                            <  2:124871/140‑1 (MQ=255)                                                                                                            ggATGTAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAGCAATGTTTTGTCTTCCGCCAGCGGCTGGCGAACGGCAGGAACGACTCCGGATg                          >  2:464019/1‑140 (MQ=255)                                                                                                             gATGTAACTTGACGTACCAGCCGGTCGGGCATTTCTCCAACGCCGCCACCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAGCAATGTTTTGTCTTCCGCCAGCGGCTGGCGAACGGCAGGAACGACTCCGGATgg                         <  2:397353/140‑1 (MQ=255)                                                                                                                 tAACATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAGCAATGTTTTGTCTTCCGCCAGCGGCTGGCGAACGGCAGGAACGACTCCGGATGGCAGa                     <  1:657112/140‑1 (MQ=255)                                                                                                                   aCATGACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAGCAATGTTTTGTCTTCCGCCAGCGGCTGGCGAACGGCAGGAACGACGCCGGATGGCAGAtt                   >  2:85605/1‑140 (MQ=255)                                                                                                                       gACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAGCAATGTTTTGTCTTCCGCCAGCGGCTGGCGAACGGCAGGAACGACTCCGGATGGCAGATTACGc               >  2:631399/1‑140 (MQ=255)                                                                                                                       gACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAGCAATGTTTTGTCTTCCGCCAGCGGCTGGCGAACGGCAGGAACGACTCCGGATGGCAGATTACGc               >  1:600692/1‑140 (MQ=255)                                                                                                                       gACGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAGCAATGTTTTGTCTTCCGCCAGCGGCTGGCCAACGGCAGGAACGGCTCCGGATGGCGGTTTAcca               >  2:492851/1‑138 (MQ=255)                                                                                                                        aCGTAATAGCCAGTCCGGCAATGCGCCAAAGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAGCAATGTTTTGTCTTCCGCCAGCGGCTGGCGAACGGCAGGAACGACTCCGGATGGCAGATTACGCa              <  1:312254/140‑1 (MQ=255)                                                                                                                         cGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAGCAATGTTTTGTCTTCCGCCAGCGGCTGGCGAACGGCAGGAACGACTCCGGATgg                         >  2:313475/1‑128 (MQ=255)                                                                                                                         cGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAGCAATGTTTTGTCTTCCGCCAGCGGCTGGCGAACGGCAGGAACGACTCCGGATgg                         <  1:313475/128‑1 (MQ=255)                                                                                                                         cGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAGCAATGTTTTGTCTTCCGCCAGCGGCTGGCGAACGGCAGGAACGACTCCGGATGGCAGATTACGCAt             >  2:556415/1‑140 (MQ=255)                                                                                                                         cGTAATAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAGCAATGTTTTGTCTTCCGCCAGCGGCTGGCGAACGGCAGGAACGACTCCGGATGGCAGATTACGCAt             >  1:92595/1‑140 (MQ=255)                                                                                                                            aaTAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAGCAATGTTTTGTCTTCCGCCAACGGCTGGCGAACTGCAAGAACGACTACGGAGGGCAGATTACGCATGCt          >  2:312254/1‑140 (MQ=255)                                                                                                                              tAGCCAGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAGCAATGTTTTGTCTTCCGCCAGCGGCTGGCGAACGGCAGGAACGACTCCGGATGGCAGATTACGCATGCttt        >  2:191511/1‑140 (MQ=255)                                                                                                                                   aGTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAGCAATGTTTTGTCTTCCGCCAGCGGCTGGCGAACGGCAGGAACGACTCCGGATGGCAGATTACGCATGCTTTTTggt   >  2:543919/1‑140 (MQ=255)                                                                                                                                    gTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAGCAATGTTTTGTCTTCCGCCAGCGGCTGGCGAACGGCAGGAACGACTCCGGATGGCAGATTACGCATGCTTTTTggtt  >  1:127809/1‑140 (MQ=255)                                                                                                                                    gTCGGGCAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAACAATGTTTTGTCTTCCGCCAGCGGCTGGCGAACGGCAGGAACGACTCCGGATGGCAGATTACGCATGCTTTTTggtt  <  2:808990/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       TGGTCACGCAGTTGGTTGTCGCAGTGCCAGCACAACACCATTGCGCCGGTACCATAACGGTGAATGACTGTTTCAGTGTGATGGTAATCGCCATTAGGCCACTGGCAGGATGTAACATGACGTAATAGCCAGTCGGACAATGCGCCAACGCCGCCAGCAGCACGAATCACCCGTTCGTTACTAAAAAACGGCAGCAATGTTTTGTCTTCCGCCAGCGGCTGGCGAACGGCAGGAACGACTCCGGATGGCAGATTACGCATGCTTTTTGGTT  >  NC_000913/1643922‑1644192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑