Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,664,627	G→A	G41D (GGC→GAC)	dmsD →	redox enzyme maturation protein DmsD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,664,627	0	G	A	100.0%	118.2 / NA	37	G41D (GGC→GAC)	dmsD	redox enzyme maturation protein DmsD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/21);  total (16/21)

TTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACTCACGCCCAGGCCTGGCAGCGTTTG  >  NC_000913/1664491‑1664730                                                                                                                                         |                                                                                                        ttCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGACTg                                                                                                      >  2:11898/1‑140 (MQ=255)         gTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGACTGGGaaa                                                                                                 <  2:586374/137‑1 (MQ=255)         gTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGACTGGGaaa                                                                                                 >  1:586374/1‑137 (MQ=255)         gTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGACTGGGAAACTc                                                                                              >  1:37560/1‑140 (MQ=255)         gTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGACTGGGAAACTc                                                                                              >  2:413638/1‑140 (MQ=255)               aaTGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTTACCAGTGACGACTGGGAAACTCAGTGGc                                                                                        <  1:510859/140‑1 (MQ=255)                 tGACCCATTTTTCACAGCAAGATAAGTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGACTGGGAAACTCAGTGGCct                                                                                      <  1:293686/140‑1 (MQ=255)                   aCCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACAACTGGGCAACTCAGTGGCctct                                                                                    >  1:342079/1‑140 (MQ=255)                    cccATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGACTGGGAAACTCAGTGGCCTCTa                                                                                   >  2:408363/1‑140 (MQ=255)                    cccATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGACTGGGAAACTCAGTGGCCTCTa                                                                                   >  1:97217/1‑140 (MQ=255)                        tttttCACAGCAAGATATTTTTCCTGTCGCGGCGCCCGTGTTGGGCGCACTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGACTGGGAAACTCAGTGGCCTCTACCAg                                                                               <  2:342079/140‑1 (MQ=255)                             cacaGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACAAGTGACGACTGGGAAACTCAGATGCCTCTACCAGAAGCg                                                                          >  2:362025/1‑140 (MQ=255)                                  cAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACAACTGGGAAACTCAGAGGCCTCAACCAGAAGCGTCAtt                                                                     >  1:394885/1‑140 (MQ=255)                                     gATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCTGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGACTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAgc                                                                  >  2:546443/1‑140 (MQ=255)                                      aTAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGACTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAgcg                                                                 <  1:11898/140‑1 (MQ=255)                                         aTTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGACTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGcgccg                                                              <  1:495322/140‑1 (MQ=255)                                               cTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGACTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTg                                                        >  1:632317/1‑140 (MQ=255)                                                 gTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGACTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGAc                                                      <  2:65037/140‑1 (MQ=255)                                                  tCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGACTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACg                                                     <  1:97099/140‑1 (MQ=255)                                                    gcggcgCGTGTGTTGGCAGCGCTGTTTTATTTCGCTCCACAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGACTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGc                                                   <  2:394885/140‑1 (MQ=255)                                                    gcggcgCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGACTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGc                                                   <  1:408363/140‑1 (MQ=255)                                                    gcggcgCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGACTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGc                                                   <  2:37560/140‑1 (MQ=255)                                                    gcggcgCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGACTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCAGGTGACGGc                                                   <  1:581279/140‑1 (MQ=255)                                                        cgcgcgTGTTGGGCGCGCTGTTTTATTACGCTCCATAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGACTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCtttt                                               <  2:473197/140‑1 (MQ=255)                                                             gtgtTGGGCGCGCTGTTTTATTACGCTCCTGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGACTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGac                                          <  1:546443/140‑1 (MQ=255)                                                             gtgtTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGACTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGac                                          <  2:632317/140‑1 (MQ=255)                                                             gtgtTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGACTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGac                                          <  2:757890/140‑1 (MQ=255)                                                              tgtTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGACTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGaca                                         <  1:528137/140‑1 (MQ=255)                                                                 tGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGACTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAg                                      <  2:589712/140‑1 (MQ=255)                                                                 tGGGCGCGCTGTTTTATAACGCGCCAGAGATTGTGTAAGCCGCACCTCAGGTTGCGGTACTCACCAGTGACGACTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAg                                      <  2:401961/140‑1 (MQ=255)                                                                                  taCGCTCCAGAGAGTGCGGAAGTCGCACCTCTGGTCGCGTTACTCACCCGTGACGACTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACTCACgt                     <  2:289551/140‑2 (MQ=255)                                                                                      cTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGACTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACt                          <  2:784462/131‑1 (MQ=255)                                                                                      cTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGACTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACt                          >  1:784462/1‑131 (MQ=255)                                                                                      cTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGACTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACTCACGCCCAg                 >  2:460782/1‑140 (MQ=255)                                                                                      cTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGACTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACTCACGCCCAg                 >  1:742052/1‑140 (MQ=255)                                                                                         cAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGACTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTTTGAAGAGACTCACGTCCAGGcc              >  1:289551/1‑140 (MQ=255)                                                                                                tGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGACTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACTCACGCCCAGGCCTGGCAGc       >  1:62438/1‑140 (MQ=255)                                                                                                     aaGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGACTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACTCGCGCCAAGGCCTGGCAGCgtttg  >  2:240643/1‑140 (MQ=255)                                                                                                                                         |                                                                                                        TTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACTCACGCCCAGGCCTGGCAGCGTTTG  >  NC_000913/1664491‑1664730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑