Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,680,569	A→G	N338S (AAC→AGC)	ydgI →	putative arginine:ornithine antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,680,569	0	A	G	100.0%	132.7 / NA	39	N338S (AAC→AGC)	ydgI	putative arginine:ornithine antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/21);  total (18/21)

AAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCG  >  NC_000913/1680450‑1680704                                                                                                                        |                                                                                                                                        aaGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTc                                                                                                                     <  1:669027/140‑1 (MQ=255) aaGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTc                                                                                                                     <  1:484200/140‑1 (MQ=255) aaGCTGGACCATCATCGCAGCACAACTACTGTTCCTGCCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTc                                                                                                                     <  1:237364/140‑1 (MQ=255)       gACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTc                                                                                                                     >  2:764763/1‑134 (MQ=255)       gACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTc                                                                                                                     <  1:764763/134‑1 (MQ=255)       gACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTCTGGTAc                                                                                                               >  1:202721/1‑140 (MQ=255)       gACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTCTGGTAc                                                                                                               >  1:749801/1‑140 (MQ=255)       gACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGAACAGCATGTGCGTGAAAATTTGTCTGGTAc                                                                                                               >  1:309290/1‑140 (MQ=255)       gACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCACCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTCTGGTAc                                                                                                               >  1:8552/1‑140 (MQ=255)         ccATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTc                                                                                                                     >  2:695034/1‑132 (MQ=255)         ccATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTc                                                                                                                     <  1:695034/132‑1 (MQ=255)            tcatGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTCTGGTACTCATc                                                                                                          >  2:155670/1‑140 (MQ=255)                    gcagAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGAc                                                                                                  <  1:720168/140‑1 (MQ=255)                      agAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCg                                                                                                >  1:136047/1‑140 (MQ=255)                           tACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCACCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTcc                                                                                           <  2:8552/140‑1 (MQ=255)                             ccGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAGCATCTGCGGGCAAATTTGTCTGGTAATCATATGGCTGACCGGTTCCGa                                                                                         >  2:225188/1‑140 (MQ=255)                                ttCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTa                                                                                      <  2:572269/140‑1 (MQ=255)                                     ggCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTacaaca                                                                                 >  2:145259/1‑140 (MQ=255)                                     ggCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTacaaca                                                                                 <  1:155670/140‑1 (MQ=255)                                      gccgccACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAacac                                                                                <  2:749801/140‑1 (MQ=255)                                       ccgccACTCATAAAGCATTCCCCCGCATTTTCGCGAGCCGGAACGCACCAGCTGCGCCATCCGCCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACg                                                                               <  2:228622/140‑1 (MQ=255)                                                  aaaGCATTCCCGCGCCTTTTTGAGCCCCATAACGCACGAGCTGCGCCATCCGCCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCAt                                                                    <  1:225188/140‑1 (MQ=255)                                                       aTTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCt                                                               <  1:408306/140‑1 (MQ=255)                                                        ttCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTc                                                              >  2:68636/1‑140 (MQ=255)                                                        ttCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTc                                                              >  1:131324/1‑140 (MQ=255)                                                         tCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTCTGGTACTGATCTGGCTAACCCGTTCCGATTACAACACGATGCTGACCATCGCCTCa                                                             >  2:257553/1‑140 (MQ=255)                                                         tCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCa                                                             >  2:186082/1‑140 (MQ=255)                                                         tCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCa                                                             >  2:24511/1‑140 (MQ=255)                                                          cccGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAg                                                            >  2:723642/1‑140 (MQ=255)                                                           ccGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGa                                                           <  1:186082/140‑1 (MQ=255)                                                                    tttGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCt                                                  <  1:47491/140‑1 (MQ=255)                                                                       gcgcgcCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGt                                               <  2:202721/140‑1 (MQ=255)                                                                        cgcgcCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTg                                              <  1:145259/140‑1 (MQ=255)                                                                          cgcCAGAACGCACAAACTGCGCTATCCGCCTCACTGTCGTTGACCAGCATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGcc                                            <  1:221730/140‑1 (MQ=255)                                                                            ccAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCt                                          <  2:131324/140‑1 (MQ=255)                                                                                                 aTCCGCCTCACTGTGGTTGACCAGCATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCt                     <  1:616426/140‑1 (MQ=255)                                                                                                   ccGCCTCTCTGTGGTTGACCAGCATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTg                   >  2:416629/1‑140 (MQ=255)                                                                                                                 ttGACCAGCATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAAc     <  1:416629/140‑1 (MQ=255)                                                                                                                    aCCAGCATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCg  >  2:193007/1‑140 (MQ=255)                                                                                                                        |                                                                                                                                        AAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCG  >  NC_000913/1680450‑1680704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑