Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,720,731	T→C	T32A (ACC→GCC)	slyA ←	DNA‑binding transcriptional dual regulator SlyA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,720,731	0	T	C	100.0%	147.8 / NA	43	T32A (ACC→GCC)	slyA	DNA‑binding transcriptional dual regulator SlyA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/20);  total (23/20)

AAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCT  >  NC_000913/1720596‑1720866                                                                                                                                        |                                                                                                                                        aaaTTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGCTtgt                                                                                                                                     >  2:686407/1‑140 (MQ=255)            ttttCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGCTTGTGTTAACTCCAg                                                                                                                          >  1:755263/1‑140 (MQ=255)                         ggTCCAGAGTACGGATCAGTGAGGGTTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGa                                                                                                             <  1:331865/140‑1 (MQ=255)                         ggTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGa                                                                                                             <  1:710800/140‑1 (MQ=255)                         ggTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGa                                                                                                             <  2:444665/140‑1 (MQ=255)                           tCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGATg                                                                                                           <  2:678413/140‑1 (MQ=255)                                     ggACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCaga                                                                                                 >  2:612297/1‑140 (MQ=255)                                     ggACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCaga                                                                                                 >  2:148690/1‑140 (MQ=255)                                      gACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCagag                                                                                                <  1:686407/140‑1 (MQ=255)                                        ccAGTGATCGCTGCTCACTGCCAATCGCTTTTGCCAGTTAAATTTGCGTCTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCa                                                                                              <  2:363643/140‑1 (MQ=255)                                          aGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACg                                                                                            >  2:761360/1‑140 (MQ=255)                                            tGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGTCTTGTGTTGACTACTGGGGTTTCAGGCGATGGTCTATCAGAGCACGcc                                                                                          >  2:401608/1‑140 (MQ=255)                                            tGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGcc                                                                                          <  1:768464/140‑1 (MQ=255)                                            tGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGcc                                                                                          >  2:768464/1‑140 (MQ=255)                                                 gctgctCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGCTTGCGTTAACTCCAGCGGTTTCAGGCGATGGTCTCTCAGAGCACGCCATATg                                                                                     >  2:285154/1‑140 (MQ=255)                                                   tgctCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATgcg                                                                                   >  2:169411/1‑140 (MQ=255)                                                     ctCGATGCCAATCGCTTTTGACAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCa                                                                                 >  1:118722/1‑140 (MQ=255)                                                               aTCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGc                                                                       <  1:761360/140‑1 (MQ=255)                                                                tCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGcc                                                                      <  1:612297/140‑1 (MQ=255)                                                                             ttGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTa                                                         >  1:806204/1‑140 (MQ=255)                                                                                  tttGCGACTGGTCTGGAGGTAACTGGTGGGTATTGTGCAACGTAACCCAATGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGc                                                    <  1:400645/140‑1 (MQ=255)                                                                                  tttGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGc                                                    <  2:806204/140‑1 (MQ=255)                                                                                                           aTGGATATTGTGTAACGTAACCCAATGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAAt                           >  1:489279/1‑140 (MQ=255)                                                                                                              gATATTGTGTAACGTAACCCAATGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAGCCTAGTGGCGAGTCTAATTTCGTCTCCTTATAATTAg                        >  1:376990/1‑140 (MQ=255)                                                                                                               gacaggtgtAACGTAACCCAATCGGCTTGGGTTAACTTCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGc                       <  1:216818/135‑1 (MQ=255)                                                                                                               atatTGTGTAACGTAACCCAATGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGc                       >  1:687691/1‑140 (MQ=255)                                                                                                               atatTGTGTAACGTAACCCAATGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGc                       <  1:536129/140‑1 (MQ=255)                                                                                                                     tgtAACGTAACCCAATGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTa                 <  1:148690/140‑1 (MQ=255)                                                                                                                       taacgtaacCCAATGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAg               >  2:695642/1‑140 (MQ=255)                                                                                                                         acgtaacCCAATGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGat             >  1:491832/1‑140 (MQ=255)                                                                                                                               cccAATGGGCTTGTGTTAACTCGAGCGGTTTCAGGCGGTGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATg       <  1:265746/140‑1 (MQ=255)                                                                                                                               cccAATGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATg       >  2:200741/1‑140 (MQ=255)                                                                                                                               cccAATGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATg       >  2:636752/1‑140 (MQ=255)                                                                                                                               cccAATGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATg       >  1:588990/1‑140 (MQ=255)                                                                                                                               cccAATGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATGTCAGCTCCTTATGATTAACTTGCTAAGatactgtc       >  2:216818/1‑135 (MQ=255)                                                                                                                               cccAATGGGCTTGTGTGAACTCAAGCGGTTGGAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATg       <  2:376990/140‑1 (MQ=255)                                                                                                                                 cAATGGGCTTGTGTTGACTCCAGCGGTTTCAGGCGATAGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCg     <  1:285154/140‑1 (MQ=255)                                                                                                                                 cAATGGGCTTGTGTTGACTCCACCGATTTAAGGCGATCGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCg     <  1:401608/140‑1 (MQ=255)                                                                                                                                 cAATGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGTGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCg     <  2:118722/140‑1 (MQ=255)                                                                                                                                    tGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCTATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCt  >  1:37225/1‑140 (MQ=255)                                                                                                                                    tGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATTTCCATATAATTACCTTGTTAAGATATTATGCGGCt  >  2:265746/1‑140 (MQ=255)                                                                                                                                    tGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCg     >  1:504803/1‑137 (MQ=255)                                                                                                                                    tGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCg     <  2:504803/137‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        AAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCT  >  NC_000913/1720596‑1720866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑