Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,735,184	T→C	S250S (AGT→AGC)	mepH →	peptidoglycan DD‑endopeptidase MepH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,735,184	0	T	C	100.0%	177.9 / NA	51	S250S (AGT→AGC)	mepH	peptidoglycan DD‑endopeptidase MepH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/27);  total (24/27)

GAACTGAAAAACGGCGACCTGGTCTTTTTCCGTACTCAGGGACGCGGCACAGCCGATCATGTCGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTGCTACCC  >  NC_000913/1735047‑1735317                                                                                                                                          |                                                                                                                                      gAACTGAAAAACGGCAACCTGGTCTGTTTACGTACTCTGGGGCGCGGCACAGCCGATCATGTCGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGCGa                                                                                                                                     <  1:296351/140‑1 (MQ=255)   aCTGAAAAACGGCGACCTGGTCTTTTTCCGTACTCAGGGGCGCGGCACAGCCGATCATGTCGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAg                                                                                                                                   <  1:446000/140‑1 (MQ=255)      gAAAAACGGCGACCTGGTCTTTTTCCGTACTCAGGGGCGCGGCACAGCCGATCATGTCGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTAACCAAATTCACATCACCTCTCTCAACGAAGact                                                                                                                                >  2:390966/1‑140 (MQ=255)       aaaaaCGGCGACCTGGTCTTTTTCCGTACTCAGGGGCGCGGCACAGCCGATCATGTCGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAGacta                                                                                                                               >  1:166394/1‑140 (MQ=255)         aaaCGGCGACCTGGTCTTTTTCCGTACTCAGGGGCGCGGCACAGCCGATCATGTCGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAGactact                                                                                                                             <  2:166394/140‑1 (MQ=255)          aaCGGCGACCTGGTCTTTTTCCGTACTCAGGGGCGCGGCACAGCCGATCATGTCGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTGAGGAAGTGCAAATCGCTTCTCGCAGCGAAGACTACTg                                                                                                                            >  1:255810/1‑140 (MQ=255)          aaCGGCGACCTGGTCTTTTTCCGTACTCAGGGGCGCGGCACAGCCGATCATGTCGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATGCAAGTCCCTTCTCCCAGCGAAGACTACTg                                                                                                                            >  1:403903/1‑140 (MQ=255)              gCGACCTGGTCTTTTTCCGTACTCAGGGGCGCGGCACAGCCGATCATGTCGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAg                                                                                                                        <  1:797626/140‑1 (MQ=255)              gCGACCTGGTCTTTTTCCGTACTCAGGGGCGCGGCACAGCCGATCATGTCGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAg                                                                                                                        <  2:406034/140‑1 (MQ=255)                 aCCTGGTCTTTTTCCGTACTCAGGGGCGCGGCACAGCCGATCATGTCGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAgcgc                                                                                                                     >  1:731679/1‑140 (MQ=255)                         tttttCCGTACTCAGAGGCGCGACAAAGCTGATCATGCCGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGt                                                                                                             <  1:390966/140‑1 (MQ=255)                                 tACTCAGGGGCGCGGCACAGCCGATCATGTCGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCATAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCtc                                                                                                     >  2:790507/1‑140 (MQ=255)                                 tACTCAGGGGCGCGGCACAGCCGATCATGTCGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCtc                                                                                                     >  2:602982/1‑140 (MQ=255)                                 tACTCAGGGGCGCGGCACAGCCGATCATGTCGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCtc                                                                                                     >  2:822512/1‑140 (MQ=255)                                 tACTCAGGGGCGCGGCACAGCCGATCATGTCGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTATCAGCGAAGACTACTGCCAGCGCCACTATGTTGGCGCtc                                                                                                     >  2:372617/1‑140 (MQ=255)                                             cgGCACAGCCGATCATGTCGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGa                                                                                         >  2:186362/1‑140 (MQ=255)                                              ggCACAGCCGATCATGTCGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGAc                                                                                        <  2:442672/140‑1 (MQ=255)                                                         tCATGTCGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAacac                                                                             <  2:573227/140‑1 (MQ=255)                                                          cATGTCGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACt                                                                            >  2:426855/1‑140 (MQ=255)                                                          cATGTCGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACt                                                                            >  1:789858/1‑140 (MQ=255)                                                          cATGTCGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTAGCGCTCGTTGGGTGATGACCCCAAAAACACt                                                                            >  2:227374/1‑140 (MQ=255)                                                               cGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACTTCGCt                                                                       >  1:407029/1‑140 (MQ=255)                                                               cGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACTTCGCt                                                                       >  2:407965/1‑140 (MQ=255)                                                               cGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCACGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGCGTAATGACCTCAAAAACACTTCGCt                                                                       >  1:344227/1‑140 (MQ=255)                                                                ggCGTGTATGTCGGCTACGGCACATATATGCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACTTCGCTa                                                                      <  1:378278/140‑1 (MQ=255)                                                                  cGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTTGCGGTGGACGGGTAATGACCCCAAAAACACTTCGCTaaa                                                                    >  2:371877/1‑140 (MQ=255)                                                                     gtATGTCGGCAACGGCAAATTTTTTCAGTCAGCACTCACAGGTCAGGATATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACTTCGCTAAAACt                                                                 <  2:344227/140‑1 (MQ=255)                                                                       aTGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACTTCGCTAAAACttt                                                               >  2:501668/1‑140 (MQ=255)                                                                       aTGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACTTCGCTAAAACttt                                                               >  2:503543/1‑140 (MQ=255)                                                                          tCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTATCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTAcc                                                            <  2:110666/140‑1 (MQ=255)                                                                                 cggcaaATTTATTCAGTCACCGCGCACAGGTCAGGTAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTTGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCtgttgt                                                     >  1:121491/1‑140 (MQ=255)                                                                                  ggcaaATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTgttgtt                                                    >  2:93020/1‑140 (MQ=255)                                                                                   gcaaATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTa                                                   >  2:710686/1‑140 (MQ=255)                                                                                        tttATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCa                                              <  2:731679/140‑1 (MQ=255)                                                                                          tATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAAc                                            <  2:588426/140‑1 (MQ=255)                                                                                             tCAGTCACCGCGCATAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAgg                                         <  1:790507/140‑1 (MQ=255)                                                                                             tCAGTCACCGCGCACGGGTCAGGAACTTCAAATCACTCCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAgg                                         <  1:372617/140‑1 (MQ=255)                                                                                             tCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAgg                                         <  1:822512/140‑1 (MQ=255)                                                                                             tCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAgg                                         <  1:602982/140‑1 (MQ=255)                                                                                             tCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAgg                                         <  1:503543/140‑1 (MQ=255)                                                                                               aGTCACCGCGCACAGGTCAGG‑AATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTa                                      <  2:522213/140‑1 (MQ=255)                                                                                                 tCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTaa                                     >  2:764124/1‑140 (MQ=255)                                                                                                  cACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACTTCGCTAAAACGTTAACCAGTTGTTGCGGCAACAGGGTAAg                                    >  1:227672/1‑140 (MQ=255)                                                                                                         cacaGGTCAGGAAATCCAAATCGCTTCTCGCAGCGAAAACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCt                             <  1:371877/140‑1 (MQ=255)                                                                                                               tCAGGAAATTCAAATCACTTCTGTCAGCGAATACTGCTGACAGCGCCATTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGtc                       <  2:227672/140‑1 (MQ=255)                                                                                                                cAGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGtct                      <  1:426855/140‑1 (MQ=255)                                                                                                                cAGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGtct                      <  1:153117/140‑1 (MQ=255)                                                                                                                 aGGAAATTCAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGtctc                     >  1:49361/1‑140 (MQ=255)                                                                                                                         cAAATCACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTa             <  1:186362/140‑1 (MQ=255)                                                                                                                              cACTTCTCTCAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTg        <  2:407029/140‑1 (MQ=255)                                                                                                                               aCTTCTCTCAGCGAACACTACTAGCAGCGCCACTATGCTGGCGCTCGTCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTGc       <  1:211601/140‑1 (MQ=255)                                                                                                                                    tctcAGCGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTGCTAccc  <  2:415748/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      GAACTGAAAAACGGCGACCTGGTCTTTTTCCGTACTCAGGGACGCGGCACAGCCGATCATGTCGGCGTGTATGTCGGCAACGGCAAATTTATTCAGTCACCGCGCACAGGTCAGGAAATTCAAATCACTTCTCTCAGTGAAGACTACTGGCAGCGCCACTATGTTGGCGCTCGTCGGGTAATGACCCCAAAAACACTTCGCTAAAACTTTACCCTGTTGTTACGGCAACAGGGTAAGTTCATCTTTTGTCTCACCTTTTAATTTGCTACCC  >  NC_000913/1735047‑1735317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑