Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,765,953 T→C E911E (GAA→GAG)  ydiJ ← putative FAD‑linked oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,765,9530TC96.9% 107.4 / NA 32E911E (GAA→GAG) ydiJputative FAD‑linked oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (16/15);  minor base G (1/0);  total (17/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTCCCTGCCATGCCGCAGCAACCCACGCTGACATTTTCCAGTTTCGCGCCAAAACGGGCAAATATCGCGGCCCATTGTGCTGGCGCACCCGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAACTCGCCACGCTCCTCGCCCAGCGCCAGTTTATATTCATCGCGATAACAAAGTACCAGCGCC  >  NC_000913/1765818‑1766088
                                                                                                                                       |                                                                                                                                       
aGTCCCTGCCATGCCGCAGCAACCCACGCTGACATTTTCCAGTTTCGCGCCAAAACGGGCAAATATCGCGGCCCATTGTGCTGGCGCACCCGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGACTCAc                                                                                                                                     >  2:590033/1‑140 (MQ=255)
     cTGCCATGCCGCAGCAACCCACGCTGACATTTTCCAGTTTCGCGCCAAAACGGGCAAATATCGCGGCCCATTGTGCTGGCGCACCCGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGACTCACCGCTg                                                                                                                                <  2:104314/140‑1 (MQ=255)
         cATGCCGCAGCAACCCACGCTGACATTTTCCAGTTTCGCGCCAAAACGGGCAAATATCGCGGCCCATTGTGCTGGCGCACCCGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGACTCACCGCTGACTg                                                                                                                            <  1:590033/140‑1 (MQ=255)
           tGCCGCAGCAACCCACGCTGACATTTTCCAGTTTCGCGCCAAAACGGGCAAATATCGCGGCCCATTGTGCTGGCGCACCCGGCAAGGCGGTAACTTCGGTACAGTGACCAGAGAGATACCATGAGTCACCGCTGACTGTa                                                                                                                          >  1:324717/1‑140 (MQ=255)
            gCCGCAGCAACCCACGCTGACATTTTCCAGTTTCGCGCCAAAACGGGCAAATATCGCGGCCCATTGTGCTGGCGCACCCGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGACTc                                                                                                                                       >  2:447736/1‑126 (MQ=255)
                      cccACGCTGTCATTTTCCAGTTTCGCGCCAAAACGGGCAAATATCGCGGCCCATTGTGCTGGCGCACCCGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGACTAACCGCTGACTGTAGCCACTGGCtg                                                                                                               <  2:701019/140‑1 (MQ=255)
                      cccACGCTGACATTTTCCAGTTTCGCGCCAAAACGGGCAAATATCGCGGCCCATTGTGCTGGCGCACCCGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGACTCACCGCTg                                                                                                                                <  2:177938/123‑1 (MQ=255)
                      cccACGCTGACATTTTCCAGTTTCGCGCCAAAACGGGCAAATATCGCGGCCCATTGTGCTGGCGCACCCGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGACTCACCGCTg                                                                                                                                >  1:177938/1‑123 (MQ=255)
                      cccACGCTGACATTTACCAGTTACGTGCCGAAACGGGGAAATATCGCGGCCCATTGTGCTGGCGCACCCGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGACTCACCGCTGACTGTAGCCACTGGCtg                                                                                                               <  1:378808/140‑1 (MQ=255)
                               aCATTTTCCAGTTTCGCGCCAAAACGGGCAAATATCGCGGCCCATTGTGCTGGCGCACCCGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGACTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAg                                                                                                      >  1:570649/1‑140 (MQ=255)
                                      ccAGTTTCGCGCCAACACGGGCTAACATCGCGGCCCAGTGTGCTGGCGCACCCGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGACTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCtt                                                                                               <  1:379748/140‑1 (MQ=255)
                                        aGTTTCGCGCCAAAACGGGCAAATATCGCGGCCCATTGTGCTGGCGCACCCGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGACTCTCCGCTGACTGTAGCTACTGGCTGTGACTCAAGTGCGCTTgc                                                                                             <  1:635288/140‑1 (MQ=255)
                                                    aaaCGGGCAAATATCGCGGCCCGTTGTGCTGGCGCCCCCCGCAAGGCGTTAACTTCGGTACAGTGACCAAAGAAATACCATGACTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCcattcatt                                                                                 <  2:324717/140‑1 (MQ=255)
                                                                            tgtgCTGGCGCACCCGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGACTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAACTCGcc                                                         >  2:779378/1‑140 (MQ=255)
                                                                            tgtgCTGGCGCACCCGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGACTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAACTCGcc                                                         >  2:205855/1‑140 (MQ=255)
                                                                                        cccGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGACTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTGATTGGGCCCTAAGACGGTAAACGCGCCACGCTCCTCGcc                                             >  2:389427/1‑140 (MQ=255)
                                                                                             cAAGGCGGTAACTTCTGTACAGCGAGCAATGAAATACCATGACTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAACTCGCCACGCTCCTCGCCCAgcg                                        <  1:389427/140‑1 (MQ=255)
                                                                                             cAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGACTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAACTCGCCACGCTCCTCGCCCAgcg                                        >  2:790682/1‑140 (MQ=255)
                                                                                             cAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGACTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAACTCGCCACGCTCCTCGCCCAgcg                                        <  2:11064/140‑1 (MQ=255)
                                                                                               aGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGACTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAACTCGCCACGCTCCTCGCCCAGCGcc                                      <  1:790682/140‑1 (MQ=255)
                                                                                               aGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGACTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAACTCGCCACGCTCCTCGCCCAGCGcc                                      <  2:570649/140‑1 (MQ=255)
                                                                                                     tAACTTCGGTACAGTGACCAAAGAAATACCATGACTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAACTCGCCACGCTCCTCGCCCAGCGCCAGTTta                                >  1:769118/1‑140 (MQ=255)
                                                                                                                 aGTGACCAAAGAAATACCATGACTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAACTCGCCACGCTCCTCGCCCAGCGCCAGTTTATATTCATCGCGa                    >  2:434372/1‑140 (MQ=255)
                                                                                                                  gTGACCAAAGAAATACCATGACTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAACTCGCCACGCTCCTCGCCCAGCGCCAGTTTATATTCATCGCGAt                   <  2:199169/140‑1 (MQ=255)
                                                                                                                   tGACCAAAGAAATACCATGACTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAACTCGCCACGCTCCTCGCCCAGCGCCAGTTTATATTCATCGCGATa                  >  2:447095/1‑140 (MQ=255)
                                                                                                                     aCCAAAGAAATACCATGACTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAACTCGCCACGCTCCTCGCCCAGCGCCAGTTTATATTCATCGCGATAAc                >  1:454867/1‑140 (MQ=255)
                                                                                                                       cAAAGAAATACCATGACTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAACTCGCCACGCTCCTCGCCCAGCGCCAGTTTATATTCATCGCGATAACaa              <  1:184535/140‑1 (MQ=255)
                                                                                                                        aaaGAAATACCATGACTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAACTCGCCACGCTCGTCGCCCAGCGCTAGCTTATATGCATCGCGATAACaaa             >  1:385281/1‑140 (MQ=255)
                                                                                                                        aaaGAAATACCATGACTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAACTCGCCACGCTCCTAGCCAAGCGCCAGTTTATGTTCATCGCGATAACaaa             >  2:309824/1‑140 (MQ=255)
                                                                                                                          aGAAATACCATGACTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAACTCGCCACGCTCCTCGCCCAGCGCCAGTTTATATTCATCGCGATAACAAAGt           >  1:755538/1‑140 (MQ=255)
                                                                                                                            aaaTACCATGACTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAACTCGCCACGCTCCTCGCCCAGCGCCAGTTTATATTCATCGCGATAACAAAGTAc         <  1:447095/140‑1 (MQ=255)
                                                                                                                                   aTGACTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAACTCGCCACGCTCCTCGCCCAGCGCCAGTTTACATTGATAGCGATAGCAAAGTACCAGCGcc  >  1:387882/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                       
AGTCCCTGCCATGCCGCAGCAACCCACGCTGACATTTTCCAGTTTCGCGCCAAAACGGGCAAATATCGCGGCCCATTGTGCTGGCGCACCCGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAACTCGCCACGCTCCTCGCCCAGCGCCAGTTTATATTCATCGCGATAACAAAGTACCAGCGCC  >  NC_000913/1765818‑1766088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑