Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,769,258	G→A	R62H (CGC→CAC)	ydiK →	putative transporter YdiK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,769,258	0	G	A	100.0%	105.7 / NA	33	R62H (CGC→CAC)	ydiK	putative transporter YdiK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/21);  total (12/21)

TCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTA  >  NC_000913/1769138‑1769394                                                                                                                         |                                                                                                                                         tcatGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCACCGCTCTCTCGCCGTTCTg                                                                                                                       >  1:11700/1‑140 (MQ=255) tcatGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCACCGCTCTCTCGCCGTTCTg                                                                                                                       >  1:467814/1‑140 (MQ=255)     gATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCACCGCTCTCTCGCCGTTCTGGtga                                                                                                                   <  2:174673/140‑1 (MQ=255)        tgtgGCATGTCTGTGCATTGTTCAAGCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCACCGCTCTCTCGCCGTTCTGGtgatga                                                                                                                <  1:342018/140‑1 (MQ=255)         gtgGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCACCGCTCTCTCGCCGTTCTGGTGATGAc                                                                                                               <  2:58634/140‑1 (MQ=255)         gtgGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCACCGCTCTCTCGCCGTTCTGGTGATGAc                                                                                                               <  2:188785/140‑1 (MQ=255)         gtgGCATGTCTGTGGATTGTTCAACCCTTTATTATTGGCATTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCACCGCTCTCTCGCCGTTCTGGTGATGAc                                                                                                               <  2:350296/140‑1 (MQ=255)          tgGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCACCGCTCTCTCGCCGTTCTGGTGATGACg                                                                                                              <  1:114014/140‑1 (MQ=255)            gCATGTCTGTGGATTGTCCAACACTTTATTCACGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCACCGCTCTCTCGCCGTTCTGGTGATGACGCt                                                                                                            <  2:346768/140‑1 (MQ=255)            gCATGTCTGTGGATTGATCAACTCTTTATTCGCCGATTTTCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCACCGCTCTCTCGCCGTTCTGGTGATGACGCt                                                                                                            <  2:404301/140‑1 (MQ=255)                    gtgGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCACCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAg                                                                                                    >  2:676882/1‑140 (MQ=255)                       gATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCACCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGa                                                                                                 <  2:733511/140‑1 (MQ=255)                       gATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCACCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGa                                                                                                 <  1:466173/140‑1 (MQ=255)                       gATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCACCGCTCTCTCGCCGCTCTGGTGATGACGCTGTTATTAGTGa                                                                                                 <  1:436299/140‑1 (MQ=255)                        aTTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCACCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGAt                                                                                                <  1:61495/140‑1 (MQ=255)                        aTTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCACCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGAt                                                                                                <  2:474539/140‑1 (MQ=255)                        aTTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCACCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGAt                                                                                                <  1:22993/140‑1 (MQ=255)                          tGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCACCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATgg                                                                                              >  2:793769/1‑140 (MQ=255)                           gTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCACCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGgt                                                                                             >  1:680634/1‑140 (MQ=255)                                 cccTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCACCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTat                                                                                       <  2:438820/140‑1 (MQ=255)                                 cccTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCACCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTat                                                                                       <  2:444262/140‑1 (MQ=255)                                 cccTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCACCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTat                                                                                       <  2:505830/140‑1 (MQ=255)                                  ccTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGTAAAAGATCATGTTTGGCCACCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTatc                                                                                      <  1:676882/140‑1 (MQ=255)                                      tATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCACCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATcc                                                                                  >  2:723666/1‑140 (MQ=255)                                      tATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCACCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATcc                                                                                  >  1:601552/1‑140 (MQ=255)                                       aTTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCACCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATccc                                                                                 <  1:723666/140‑1 (MQ=255)                                                                   tggtTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCACCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTATATCATCTCTAGTGGTTTGCTAGTTAACAGTAtcgtc                                                     >  1:216221/1‑140 (MQ=255)                                                                             cACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCACCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCg                                           <  2:467814/140‑1 (MQ=255)                                                                               ccTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCACCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGc                                         >  1:537081/1‑140 (MQ=255)                                                                               ccTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCACCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGc                                         >  2:514660/1‑140 (MQ=255)                                                                                             ttACGTTTGCAAAAGATCATGTTTGGCCACCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGCTAATTAAAGc                           >  1:708625/1‑140 (MQ=255)                                                                                                        aaaGATCATGTTTGGCCACCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCg                <  2:601552/140‑1 (MQ=255)                                                                                                                     ggCCACCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGtt   >  2:406652/1‑140 (MQ=255)                                                                                                                      gccaccGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGGTAATTAAAGCCATTTCCAGCGGTGACATGACGTTa  >  2:474359/1‑140 (MQ=255)                                                                                                                         |                                                                                                                                         TCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTA  >  NC_000913/1769138‑1769394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑