Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,785,140	A→G	V658A (GTT→GCT)	ppsA ←	phosphoenolpyruvate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,785,140	0	A	G	100.0%	153.8 / NA	44	V658A (GTT→GCT)	ppsA	phosphoenolpyruvate synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/31);  total (13/31)

GTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGTCCCATGTCGTTGCGCACACGTTTCACTGCTTCACACTCCAGCGCGAAACAGTCGCGGAAG  >  NC_000913/1785004‑1785262                                                                                                                                         |                                                                                                                           gTTTGAGCCAATTGACAAGCCGACGCAATCTTCGAGGTACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAgcca                                                                                                                         <  1:217981/140‑1 (MQ=255)  tttGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTCTCAGCCCCTGACGCGCCAGTTCTTCAgccag                                                                                                                        >  2:349284/1‑139 (MQ=255)         cAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTc                                                                                                                 <  2:730749/140‑1 (MQ=255)           aTTGAGAAGCCGTCGAATTATTCGAGGAACTCCTCAGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGc                                                                                                               <  2:264145/140‑1 (MQ=255)           aTTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCTGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGc                                                                                                               <  2:246370/140‑1 (MQ=255)           aTTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGc                                                                                                               <  1:823993/140‑1 (MQ=255)           aTTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGc                                                                                                               <  1:118001/140‑1 (MQ=255)           aTTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGc                                                                                                               <  1:205771/140‑1 (MQ=255)           aTTGAGAAGCCGTCGAAATATTCGACGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGc                                                                                                               <  1:286689/140‑1 (MQ=255)           aTTGAGAAACCGTCGATATATTCGAGGAACTGCTCCGCCCGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGc                                                                                                               <  2:396376/140‑1 (MQ=255)            ttGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGcc                                                                                                              >  2:153267/1‑140 (MQ=255)             tGAGAAGCCGTCGAAATCTTCGAGGAACTGCTCGGTCAGGAAGGCGTTTGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGCCt                                                                                                             <  2:376461/140‑1 (MQ=255)                gaAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGGTCCTCAGCCGCCGCTTTCGCCTGAt                                                                                                          >  1:258307/1‑140 (MQ=255)                   gCCGTCGAAATATTCAAGGAACCGCTCGGTCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGCCTGATCTa                                                                                                       <  1:251656/140‑1 (MQ=255)                      gTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGCCTGATCTACgg                                                                                                    >  2:109019/1‑140 (MQ=255)                      gTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGCCTGATCTACgg                                                                                                    >  2:578854/1‑140 (MQ=255)                            atatTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGCCTGATCTACGGTACGCa                                                                                              <  2:639758/140‑1 (MQ=255)                              atTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGATGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGCCTGATCTACGGTACGCACg                                                                                            <  1:100760/140‑1 (MQ=255)                              atTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGCCTGATCTACGGTACGCACg                                                                                            >  2:703955/1‑140 (MQ=255)                                tCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGCCTGATCTACGGTACGCACGaa                                                                                          <  1:22280/140‑1 (MQ=255)                                tCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGCCTGATCTACGGTACGCACGaa                                                                                          <  1:75369/140‑1 (MQ=255)                                tCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGCCTGATCTACGGTACGCACGaa                                                                                          <  1:797126/140‑1 (MQ=255)                                  gAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGCCTGATCTACGGTACGCACGAACg                                                                                        <  1:109019/140‑1 (MQ=255)                                    ggAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGCCTGATCTACGGTACGCACGAACggg                                                                                      <  2:540162/140‑1 (MQ=255)                                       aCTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATc                                                                                   >  2:493026/1‑140 (MQ=255)                                         tGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTGTTCAGCCACCGCTTTCGCCTGATCTGCGGTACGCACGAACGGGATCAt                                                                                 >  2:250215/1‑140 (MQ=255)                                                ccAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGCCTGATCTACGGTACGCACGaa                                                                                          <  1:157403/124‑1 (MQ=255)                                                ccAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGCCTGATCTACGGTACGCACGaa                                                                                          >  2:157403/1‑124 (MQ=255)                                                   gCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACg                                                                       >  2:783455/1‑140 (MQ=255)                                                   gCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACg                                                                       <  1:703955/140‑1 (MQ=255)                                                        gCGTTGGACGGGATTTCTCACATCATGATGATTTTTAGCACGTTCTCGTCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGt                                                                  <  2:258307/140‑1 (MQ=255)                                                        gCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGt                                                                  <  1:638458/140‑1 (MQ=255)                                                        gCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGt                                                                  <  1:622218/140‑1 (MQ=255)                                                        gCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGt                                                                  <  2:434533/140‑1 (MQ=255)                                                        gCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGt                                                                  <  1:191988/140‑1 (MQ=255)                                                               aCGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGTccc                                                           <  1:493026/140‑1 (MQ=255)                                                                             atcatGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGTCCCATGTCGTTGCGcac                                             >  1:667783/1‑140 (MQ=255)                                                                              tcatGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGTCCCATGTCGTTGCGcaca                                            >  1:114081/1‑140 (MQ=255)                                                                              tcatGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGTCCCATGTCGTTGCGcaca                                            <  1:153267/140‑1 (MQ=255)                                                                                   atgatTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGTCCCAt                                                         >  1:103644/1‑122 (MQ=255)                                                                                   atgatTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGTCCCAt                                                         <  2:103644/122‑1 (MQ=255)                                                                                     gatTTTCAGCCCGTTATCGCCATGTATCAGCCCCTGAAGCGCCAGTTCTTCAGCCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGTCCCATGTCGTTGCGCACACGTTTCa                                     <  1:388995/140‑1 (MQ=255)                                                                                        tttCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGTCCCATGTCGTTGCGCACACGTTTCACTg                                  <  1:413263/140‑1 (MQ=255)                                                                                           cAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGTCCCATGTCGTTGCGCACACGTTTCACTGCtt                               <  1:38364/140‑1 (MQ=255)                                                                                                  ttCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGTCCCATGTCGTTGCGCACACGTTTCACTGCTTCACACTc                        <  1:783455/140‑1 (MQ=255)                                                                                                  ttCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAGCCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGTCCCATGTCGTTGCGCACACGTTTCACTGCTTCACACTc                        >  2:154627/1‑140 (MQ=255)                                                                                                                        gACGCGCCAGTTCTTCAGCCACCGCATTCCCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGTCCCATGTCGTTGCGCACACGTTTCACTGCTTCACACTCCAGCGCGAAACAGTCGCGGAAg  <  1:250215/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                           GTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGTCCCATGTCGTTGCGCACACGTTTCACTGCTTCACACTCCAGCGCGAAACAGTCGCGGAAG  >  NC_000913/1785004‑1785262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑