Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,792,029	T→C	intergenic (‑9/+238)	ydiV ← / ← nlpC	anti‑FlhDC factor/NlpC/P60 family lipoprotein NlpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,792,029	0	T	C	100.0%	144.7 / NA	43	intergenic (‑9/+238)	ydiV/nlpC	anti‑FlhDC factor/NlpC/P60 family lipoprotein NlpC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/21);  total (22/21)

TACGAACCGTACCATCTTCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATC  >  NC_000913/1791894‑1792159                                                                                                                                        |                                                                                                                                   tACGAACCGTACCATCTGCACTTGAGAAATGTGTATTTATCTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAAGCAGAATGATAAAGTTTCTCCAAAAAAATCTTCATGGTAACCGCCCct                                                                                                                                <  2:289416/140‑1 (MQ=255)          tACCATCTTCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGGTAAAGATTTTCCGAAAGAATCTTCAAGGTATCCGCCCCTCTCGAAGGa                                                                                                                       >  2:253668/1‑140 (MQ=255)             cATCTTCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGAAAAAGATTCTCCAAAAAAATCTTCATGGTAACCGCCCCTCTCGAAGGATGt                                                                                                                    <  2:186625/140‑1 (MQ=255)                       ggAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTAt                                                                                                          <  2:721617/140‑1 (MQ=255)                       ggAGAAATGGGTAATCAACTCATCGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTAt                                                                                                          <  1:253668/140‑1 (MQ=255)                                  tAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGCCCCTCTCTAAGGATGTTTTTCCGTATCCGGCTAAAAg                                                                                               >  1:324695/1‑140 (MQ=255)                                  tAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAg                                                                                               >  2:84178/1‑140 (MQ=255)                                  tAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTAATGGTAACCGCCTCTCGCGAAGGATTTTTTCGAGTATCCGGCTAAAAg                                                                                               >  2:364184/1‑140 (MQ=255)                                      tAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGAt                                                                                           <  1:158665/140‑1 (MQ=255)                                        aCTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGa                                                                                         <  1:802172/140‑1 (MQ=255)                                        aCTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCAGGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGa                                                                                         <  1:117073/140‑1 (MQ=255)                                           cAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGCCCCTCTCGTAGGATGTTTTTAAGGATCCGGATAAAAGGGATGAGGc                                                                                      >  1:390043/1‑140 (MQ=255)                                            aaCGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGcc                                                                                     >  1:5337/1‑140 (MQ=255)                                                   aCAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGAc                                                                              >  2:587236/1‑140 (MQ=255)                                                   aCAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGAc                                                                              <  1:442892/140‑1 (MQ=255)                                                     aaGGACTTGCCGTTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATAAAAAAGATTCCCCAAAAAAATCTTCATGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACAt                                                                            <  2:90348/140‑1 (MQ=255)                                                        gACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAAc                                                                         <  1:587236/140‑1 (MQ=255)                                                             gCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTa                                                                    >  1:500604/1‑140 (MQ=255)                                                               tGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGAc                                                                              >  2:665941/1‑128 (MQ=255)                                                               tGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATAATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGAc                                                                              <  1:665941/128‑1 (MQ=255)                                                                 tttGTCTCTTATCGGCAGAAAAAAGCAACCAGAATGATAAAGATTCTCCAAAAAAATCTCCATGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAGCAATTACAGa                                                                <  2:5337/139‑1 (MQ=255)                                                                  ttGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGaa                                                               >  2:746211/1‑140 (MQ=255)                                                                  ttGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCCAAAAGGGAAAAGGCCATAAGAAATAACCATTACAGaa                                                               >  1:397872/1‑140 (MQ=255)                                                                      ctctTATCGGCAGGATATAGCATTCGGAACGATAAAGCTTCTCCAAAAAAATCTTCATGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAg                                                           <  1:364184/140‑1 (MQ=255)                                                                       tctTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGt                                                          <  1:746211/140‑1 (MQ=255)                                                                                gaGGAAAAAGCAATCAGAATGACAAATATTCCCCAAAAACATCTTCAGGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCAt                                                 <  2:324695/139‑1 (MQ=255)                                                                                cAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCAt                                                 >  1:527100/1‑140 (MQ=255)                                                                                cAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCAt                                                 >  1:434762/1‑140 (MQ=255)                                                                                   gAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATGACAATTCCAGAAGAAGTAACTTTCATTTg                                              >  1:368609/1‑140 (MQ=255)                                                                                        aGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCa                                         >  1:428862/1‑140 (MQ=255)                                                                                             tCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAGTTATAGAAGGAGGAACTCTCATTTGTTCCATGTTa                                    >  2:245366/1‑140 (MQ=255)                                                                                             tCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTa                                    >  2:70152/1‑140 (MQ=255)                                                                                                aaaTGATACAGATTCTCAAAAATAATCTTCACGGTCACCCCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAAcc                                 <  2:368609/139‑1 (MQ=255)                                                                                                           aTTCTCCAAAAAAATCTTCATGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAgg                      >  2:586515/1‑140 (MQ=255)                                                                                                           aTTCTCCAAAAAAATCTTCATGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAgg                      >  1:585323/1‑140 (MQ=255)                                                                                                              ctcCAAAAAAATCTTCATGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGt                   >  2:731042/1‑140 (MQ=255)                                                                                                               tcCAAAAAAATCTTCATGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTc                  >  1:445189/1‑140 (MQ=255)                                                                                                                 cAAAAAAATCTTCATGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCt                <  1:586515/140‑1 (MQ=255)                                                                                                                           ttCATGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGAt      >  2:47045/1‑140 (MQ=255)                                                                                                                              aTGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGAGTAt   <  2:428862/140‑1 (MQ=255)                                                                                                                               tGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATc  <  1:47045/140‑1 (MQ=255)                                                                                                                               tGGTAACCGCCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATc  <  1:731042/140‑1 (MQ=255)                                                                                                                               tGGTAACCACCCCTCTCTAAGGCTGTTTTTTCGTAACCGTCTAAAAGGTATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATc  <  2:390043/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                   TACGAACCGTACCATCTTCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATC  >  NC_000913/1791894‑1792159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑