Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,806,684	T→C	R105R (CGT→CGC)	pfkB →	6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,806,684	0	T	C	100.0%	75.7 / NA	23	R105R (CGT→CGC)	pfkB	6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/10);  total (13/10)

GCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAACC  >  NC_000913/1806566‑1806819                                                                                                                       |                                                                                                                                        gCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGCTTTGTTATGCCAGGCGCGGCa                                                                                                                    <  1:629918/140‑1 (MQ=255)                   tGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGCTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGttt                                                                                                  >  1:130563/1‑140 (MQ=255)                   tGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGCTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGttt                                                                                                  >  1:2740/1‑140 (MQ=255)                     ttGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGCTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCg                                                                                                <  2:579974/140‑1 (MQ=255)                                   tGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGCTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGc                                                                                  <  1:487458/140‑1 (MQ=255)                                         cGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGCTTTGTTATGCCAGGCGCGACATTAAATGAAGCTGAGTTACGCCAGATTGAAGAGCAAGTTc                                                                            >  1:288746/1‑140 (MQ=255)                                                      gAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGCTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATc                                                               >  1:711191/1‑140 (MQ=255)                                                         gCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGCTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGAAAGTCCTGGAAATAGAATCCgg                                                            >  2:384404/1‑140 (MQ=255)                                                          ccAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGCTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCggg                                                           <  2:130563/140‑1 (MQ=255)                                                                          gCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGCTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATaa                                           >  2:200680/1‑140 (MQ=255)                                                                                  tACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGCTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGc                                   <  1:552024/140‑1 (MQ=255)                                                                                    cacGTACATGTGGAATCAAGCGATGTGCACTATCGCTATGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCt                                 <  1:384404/140‑1 (MQ=255)                                                                                        tACATGTGGAAGCAAGCGGTGAGCAGTATCGCTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTgccg                             <  2:711191/140‑1 (MQ=255)                                                                                        tACATGTGGAAGCAAGCGGTGAGCAGTATCGCTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTACTGGAAATGGAAACCGCGGCCATCCAGGTCATAAGCGGAAGCCTgccg                             >  2:253986/1‑140 (MQ=255)                                                                                               ggAAGCAAGCGGTGAGCAGTATCGCTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGgtg                      >  2:450133/1‑140 (MQ=255)                                                                                                 aagcaagcGGTGAGCAGTATCGCTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGtgtg                    >  2:447961/1‑140 (MQ=255)                                                                                                  agcaagcGGTGAGCAGTATCGCTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTAAATCCGGGTCCAACCTGTTCATAAGTGGAATACTGCCGCGAGGTGTGa                   >  2:404557/1‑140 (MQ=255)                                                                                                    caagcGGTGAGCAGTATCGCTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAg                 <  2:648707/140‑1 (MQ=255)                                                                                                     aagcGGTGAGCAGTATCGCTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGc                >  1:482964/1‑140 (MQ=255)                                                                                                     aagcGGTGAGCAGTATCGCTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGc                >  1:785418/1‑140 (MQ=255)                                                                                                       gcGGTGAGCAGTATCGCTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTg              >  1:142337/1‑140 (MQ=255)                                                                                                                   aTCGCTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAAcc  <  1:200680/140‑1 (MQ=255)                                                                                                                   aTCGCTTTGTTATGCCAGACGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAAcc  <  2:759401/140‑1 (MQ=255)                                                                                                                       |                                                                                                                                        GCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAACC  >  NC_000913/1806566‑1806819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑